Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause
Imuno-histomorfometrijske i –fluorescentne karakteristike gh ćelija nakon tretmana genisteinom ili daidzeinom u animalnom modelu andropauze
Аутори
Ajdžanović, VladimirMedigović, Ivana
Živanović, Jasmina
Šošić-Jurjević, Branka
Trifunović, Svetlana
Tanić, Nasta
Milošević, Verica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Somatopause, the complex aspect of andropause, is recognizable by reduced growth hormone - GH/insulin-like growth factor 1 axis function in the ageing male. Soy isoflavones (usually genistein and daidzein), which are known for their beneficial effects in the treatment of ageing symptoms, are active in the pituitary, as well. The immunohistomorphometric and -fluorescent characteristics of pituitary growth hormone secreting cells, in an animal model of andropause, were examined after a treatment with genistein or daidzein. Andropausal Wistar rats were divided into sham operated, orchidectomized and genistein or daidzein treated orchidectomized groups. Genistein or daidzein (30 mg/kg/day) were administered subcutaneously for three weeks, while sham operated and orchidectomized groups received the vehicle alone. Growth hormone secreting cells were identified by the peroxidase-antiperoxidase immuno-histochemical, and immuno-fluorescent procedure. The main characteristic of growth hormone se...creting cells in soy isoflavones treated groups is a weaker immuno-histochemical staining and immuno-fluorescent signal compared to sham operated and orchidectomized groups. The growth hormone secreting cell volume in orchidectomized +genistein or +daidzein groups is by 13.8% and 11.9% (p<0.05) smaller respectively, in comparison with the orchidectomized group. In orchidectomized +genistein or +daidzein groups, the growth hormone secreting cells relative volume density is by 62.5% and 61.0% lower (p<0.05)
Somatopauza, kao kompleksan aspekt andropauze, je prepoznatljiva po sniženom nivou funkcije somatotropne - GH ose kod starih mužjaka. Izofl avoni soje (najčešće genistein i daidzein), imaju blagotvorne efekte pri tretmanu simptoma starenja, a ispoljavaju svoje dejstvo i na nivou hipofi ze. Imuno-histomorfometrijske i – fl uorescentne karakteristike hipofi znih somatotropnih ćelija su ispitivane nakon tretmana genisteinom ili daidzeinom, u animalnom modelu andropauze. Pacovi Wistar soja u andropauzi su podeljeni u nekoliko grupa: prividno operisanu, orhidektomisanu i genisteinom odnosno daidzeinom tretiranu orhidektomisanu grupu. Rastvoreni genistein, odnosno daidzein (30 mg/kg/dan) su potkožno davani tokom tri nedelje, dok su prividno operisana i orhidektomisana grupa dobijale samo rastvarač tokom istog perioda. Somatotropne ćelije su identifi kovane peroksidaza-antiperoksidaza imuno-histohemijskom, i imunofl uorescentnom metodom. Osnovna karakteristika somatotropnih ćelija u grupama t...retiranim izofl avonima soje je slabije imuno-histohemijsko bojenje i slabiji imuno– fl uorescentni signal u poređenju sa prividno operisanom i orhidektomisanom grupom. Volumen somatotropnih ćelija u orhidektomisanoj i genisteinom, odnosno daidzeinom tretiranoj grupi je za 13,8%, odnosno 11,9% (p<0,05) smanjen u poređenju sa orhidektomisanom grupom. U orhidektomisanoj i genisteinom, odnosno daidzeinom tretiranoj grupi volumenska gustina somatotropnih ćelija je za 62,5%, odnosno 61,0% niža (p<0,05) nego kod prividno operisane grupe, te za 65,4%, odnosno 64,0% (p<0,05) niža u poređenju sa orhidektomisanom grupom. Može se zaključiti da hronični tretman genisteinom ili daidzeinom, u animalnom modelu andropauze, ima negativan efekat na imuno-histomorfometrijske i – fl uorescentne karakteristike somatotropnih ćelija.
Кључне речи:
andropause / GH cells / immuno-fluorescence / immuno-histochemistry / soy isoflavonesИзвор:
Acta Veterinaria-Beograd, 2014, 64, 1, 93-104Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Одговор неуроендокриног система пацова на одабране биљне екстракте, фитоестрогене, стероидне и пептидне хормоне (RS-MESTD-Basic Research (BR or ON)-173009)
DOI: 10.2478/acve-2014-0010
ISSN: 0567-8315
WoS: 000341341000010
Scopus: 2-s2.0-84902456818
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Ajdžanović, Vladimir AU - Medigović, Ivana AU - Živanović, Jasmina AU - Šošić-Jurjević, Branka AU - Trifunović, Svetlana AU - Tanić, Nasta AU - Milošević, Verica PY - 2014 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2145 AB - Somatopause, the complex aspect of andropause, is recognizable by reduced growth hormone - GH/insulin-like growth factor 1 axis function in the ageing male. Soy isoflavones (usually genistein and daidzein), which are known for their beneficial effects in the treatment of ageing symptoms, are active in the pituitary, as well. The immunohistomorphometric and -fluorescent characteristics of pituitary growth hormone secreting cells, in an animal model of andropause, were examined after a treatment with genistein or daidzein. Andropausal Wistar rats were divided into sham operated, orchidectomized and genistein or daidzein treated orchidectomized groups. Genistein or daidzein (30 mg/kg/day) were administered subcutaneously for three weeks, while sham operated and orchidectomized groups received the vehicle alone. Growth hormone secreting cells were identified by the peroxidase-antiperoxidase immuno-histochemical, and immuno-fluorescent procedure. The main characteristic of growth hormone secreting cells in soy isoflavones treated groups is a weaker immuno-histochemical staining and immuno-fluorescent signal compared to sham operated and orchidectomized groups. The growth hormone secreting cell volume in orchidectomized +genistein or +daidzein groups is by 13.8% and 11.9% (p<0.05) smaller respectively, in comparison with the orchidectomized group. In orchidectomized +genistein or +daidzein groups, the growth hormone secreting cells relative volume density is by 62.5% and 61.0% lower (p<0.05) AB - Somatopauza, kao kompleksan aspekt andropauze, je prepoznatljiva po sniženom nivou funkcije somatotropne - GH ose kod starih mužjaka. Izofl avoni soje (najčešće genistein i daidzein), imaju blagotvorne efekte pri tretmanu simptoma starenja, a ispoljavaju svoje dejstvo i na nivou hipofi ze. Imuno-histomorfometrijske i – fl uorescentne karakteristike hipofi znih somatotropnih ćelija su ispitivane nakon tretmana genisteinom ili daidzeinom, u animalnom modelu andropauze. Pacovi Wistar soja u andropauzi su podeljeni u nekoliko grupa: prividno operisanu, orhidektomisanu i genisteinom odnosno daidzeinom tretiranu orhidektomisanu grupu. Rastvoreni genistein, odnosno daidzein (30 mg/kg/dan) su potkožno davani tokom tri nedelje, dok su prividno operisana i orhidektomisana grupa dobijale samo rastvarač tokom istog perioda. Somatotropne ćelije su identifi kovane peroksidaza-antiperoksidaza imuno-histohemijskom, i imunofl uorescentnom metodom. Osnovna karakteristika somatotropnih ćelija u grupama tretiranim izofl avonima soje je slabije imuno-histohemijsko bojenje i slabiji imuno– fl uorescentni signal u poređenju sa prividno operisanom i orhidektomisanom grupom. Volumen somatotropnih ćelija u orhidektomisanoj i genisteinom, odnosno daidzeinom tretiranoj grupi je za 13,8%, odnosno 11,9% (p<0,05) smanjen u poređenju sa orhidektomisanom grupom. U orhidektomisanoj i genisteinom, odnosno daidzeinom tretiranoj grupi volumenska gustina somatotropnih ćelija je za 62,5%, odnosno 61,0% niža (p<0,05) nego kod prividno operisane grupe, te za 65,4%, odnosno 64,0% (p<0,05) niža u poređenju sa orhidektomisanom grupom. Može se zaključiti da hronični tretman genisteinom ili daidzeinom, u animalnom modelu andropauze, ima negativan efekat na imuno-histomorfometrijske i – fl uorescentne karakteristike somatotropnih ćelija. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause T1 - Imuno-histomorfometrijske i –fluorescentne karakteristike gh ćelija nakon tretmana genisteinom ili daidzeinom u animalnom modelu andropauze VL - 64 IS - 1 SP - 93 EP - 104 DO - 10.2478/acve-2014-0010 ER -
@article{ author = "Ajdžanović, Vladimir and Medigović, Ivana and Živanović, Jasmina and Šošić-Jurjević, Branka and Trifunović, Svetlana and Tanić, Nasta and Milošević, Verica", year = "2014", abstract = "Somatopause, the complex aspect of andropause, is recognizable by reduced growth hormone - GH/insulin-like growth factor 1 axis function in the ageing male. Soy isoflavones (usually genistein and daidzein), which are known for their beneficial effects in the treatment of ageing symptoms, are active in the pituitary, as well. The immunohistomorphometric and -fluorescent characteristics of pituitary growth hormone secreting cells, in an animal model of andropause, were examined after a treatment with genistein or daidzein. Andropausal Wistar rats were divided into sham operated, orchidectomized and genistein or daidzein treated orchidectomized groups. Genistein or daidzein (30 mg/kg/day) were administered subcutaneously for three weeks, while sham operated and orchidectomized groups received the vehicle alone. Growth hormone secreting cells were identified by the peroxidase-antiperoxidase immuno-histochemical, and immuno-fluorescent procedure. The main characteristic of growth hormone secreting cells in soy isoflavones treated groups is a weaker immuno-histochemical staining and immuno-fluorescent signal compared to sham operated and orchidectomized groups. The growth hormone secreting cell volume in orchidectomized +genistein or +daidzein groups is by 13.8% and 11.9% (p<0.05) smaller respectively, in comparison with the orchidectomized group. In orchidectomized +genistein or +daidzein groups, the growth hormone secreting cells relative volume density is by 62.5% and 61.0% lower (p<0.05), Somatopauza, kao kompleksan aspekt andropauze, je prepoznatljiva po sniženom nivou funkcije somatotropne - GH ose kod starih mužjaka. Izofl avoni soje (najčešće genistein i daidzein), imaju blagotvorne efekte pri tretmanu simptoma starenja, a ispoljavaju svoje dejstvo i na nivou hipofi ze. Imuno-histomorfometrijske i – fl uorescentne karakteristike hipofi znih somatotropnih ćelija su ispitivane nakon tretmana genisteinom ili daidzeinom, u animalnom modelu andropauze. Pacovi Wistar soja u andropauzi su podeljeni u nekoliko grupa: prividno operisanu, orhidektomisanu i genisteinom odnosno daidzeinom tretiranu orhidektomisanu grupu. Rastvoreni genistein, odnosno daidzein (30 mg/kg/dan) su potkožno davani tokom tri nedelje, dok su prividno operisana i orhidektomisana grupa dobijale samo rastvarač tokom istog perioda. Somatotropne ćelije su identifi kovane peroksidaza-antiperoksidaza imuno-histohemijskom, i imunofl uorescentnom metodom. Osnovna karakteristika somatotropnih ćelija u grupama tretiranim izofl avonima soje je slabije imuno-histohemijsko bojenje i slabiji imuno– fl uorescentni signal u poređenju sa prividno operisanom i orhidektomisanom grupom. Volumen somatotropnih ćelija u orhidektomisanoj i genisteinom, odnosno daidzeinom tretiranoj grupi je za 13,8%, odnosno 11,9% (p<0,05) smanjen u poređenju sa orhidektomisanom grupom. U orhidektomisanoj i genisteinom, odnosno daidzeinom tretiranoj grupi volumenska gustina somatotropnih ćelija je za 62,5%, odnosno 61,0% niža (p<0,05) nego kod prividno operisane grupe, te za 65,4%, odnosno 64,0% (p<0,05) niža u poređenju sa orhidektomisanom grupom. Može se zaključiti da hronični tretman genisteinom ili daidzeinom, u animalnom modelu andropauze, ima negativan efekat na imuno-histomorfometrijske i – fl uorescentne karakteristike somatotropnih ćelija.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause, Imuno-histomorfometrijske i –fluorescentne karakteristike gh ćelija nakon tretmana genisteinom ili daidzeinom u animalnom modelu andropauze", volume = "64", number = "1", pages = "93-104", doi = "10.2478/acve-2014-0010" }
Ajdžanović, V., Medigović, I., Živanović, J., Šošić-Jurjević, B., Trifunović, S., Tanić, N.,& Milošević, V.. (2014). Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(1), 93-104. https://doi.org/10.2478/acve-2014-0010
Ajdžanović V, Medigović I, Živanović J, Šošić-Jurjević B, Trifunović S, Tanić N, Milošević V. Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause. in Acta Veterinaria-Beograd. 2014;64(1):93-104. doi:10.2478/acve-2014-0010 .
Ajdžanović, Vladimir, Medigović, Ivana, Živanović, Jasmina, Šošić-Jurjević, Branka, Trifunović, Svetlana, Tanić, Nasta, Milošević, Verica, "Immuno-histomorphometric and -fluorescent characteristics of GH cells after treatment with genistein or daidzein in an animal model of andropause" in Acta Veterinaria-Beograd, 64, no. 1 (2014):93-104, https://doi.org/10.2478/acve-2014-0010 . .