Enhanced sister-chromatid exchange rate in human lymphocytes exposed to 17 beta estradiol in vitro
Abstract
Background. Epidemiologic data and animal experiments strongly implicate that steroid hormones are involved in the process of malignant transformation due to their capability to stimulate mitotic division and/or elevate the level of Mutations in susceptible cells. Methods. The objectives of this investigation were to evaluate the effects of 17beta estradiol in sister-chromatid exchange (SCE) test on cultured human peripheral blood lymphocytes. The lowest concentration of 17beta estradiol used in this experiment (10(-10) M) was calculated as comparable with the physiologic blood level of 17beta estradiol in women. Three experimental concentrations corresponded to minimal (7 X 10(-8) M), average (3.5 X 10(-6) M), and maximal (7 X 10(-6) M) therapeutic doses in human medicine. In addition, the highest concentrations exceed maximal therapeutic dose 10-fold (7 X 10(-5) M) and 30-fold (2.1 X 10 (4) M), respectively. Results. The obtained results indicate that estradiol significantly elevates... SCE per cell frequency tit all concentrations applied except at the lowest one. However. estradiol has not influenced mitotic activity of cultured human lymphocytes significantly. Conclusions. It can be concluded that 17beta estradiol expressed genotoxic effects and therefore fright represent a human health risk.
Keywords:
estradiol / genotoxicity / sister-chromatid exchange / cancer / lymphocyteSource:
Archives of Medical Research, 2002, 33, 2, 148-151Publisher:
- Elsevier Science Inc, New York
DOI: 10.1016/S0188-4409(01)00355-1
ISSN: 0188-4409
PubMed: 11886713
WoS: 000174435800009
Scopus: 2-s2.0-0036124757
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Đelić, Ninoslav AU - Đelić, D PY - 2002 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/217 AB - Background. Epidemiologic data and animal experiments strongly implicate that steroid hormones are involved in the process of malignant transformation due to their capability to stimulate mitotic division and/or elevate the level of Mutations in susceptible cells. Methods. The objectives of this investigation were to evaluate the effects of 17beta estradiol in sister-chromatid exchange (SCE) test on cultured human peripheral blood lymphocytes. The lowest concentration of 17beta estradiol used in this experiment (10(-10) M) was calculated as comparable with the physiologic blood level of 17beta estradiol in women. Three experimental concentrations corresponded to minimal (7 X 10(-8) M), average (3.5 X 10(-6) M), and maximal (7 X 10(-6) M) therapeutic doses in human medicine. In addition, the highest concentrations exceed maximal therapeutic dose 10-fold (7 X 10(-5) M) and 30-fold (2.1 X 10 (4) M), respectively. Results. The obtained results indicate that estradiol significantly elevates SCE per cell frequency tit all concentrations applied except at the lowest one. However. estradiol has not influenced mitotic activity of cultured human lymphocytes significantly. Conclusions. It can be concluded that 17beta estradiol expressed genotoxic effects and therefore fright represent a human health risk. PB - Elsevier Science Inc, New York T2 - Archives of Medical Research T1 - Enhanced sister-chromatid exchange rate in human lymphocytes exposed to 17 beta estradiol in vitro VL - 33 IS - 2 SP - 148 EP - 151 DO - 10.1016/S0188-4409(01)00355-1 ER -
@article{ author = "Đelić, Ninoslav and Đelić, D", year = "2002", abstract = "Background. Epidemiologic data and animal experiments strongly implicate that steroid hormones are involved in the process of malignant transformation due to their capability to stimulate mitotic division and/or elevate the level of Mutations in susceptible cells. Methods. The objectives of this investigation were to evaluate the effects of 17beta estradiol in sister-chromatid exchange (SCE) test on cultured human peripheral blood lymphocytes. The lowest concentration of 17beta estradiol used in this experiment (10(-10) M) was calculated as comparable with the physiologic blood level of 17beta estradiol in women. Three experimental concentrations corresponded to minimal (7 X 10(-8) M), average (3.5 X 10(-6) M), and maximal (7 X 10(-6) M) therapeutic doses in human medicine. In addition, the highest concentrations exceed maximal therapeutic dose 10-fold (7 X 10(-5) M) and 30-fold (2.1 X 10 (4) M), respectively. Results. The obtained results indicate that estradiol significantly elevates SCE per cell frequency tit all concentrations applied except at the lowest one. However. estradiol has not influenced mitotic activity of cultured human lymphocytes significantly. Conclusions. It can be concluded that 17beta estradiol expressed genotoxic effects and therefore fright represent a human health risk.", publisher = "Elsevier Science Inc, New York", journal = "Archives of Medical Research", title = "Enhanced sister-chromatid exchange rate in human lymphocytes exposed to 17 beta estradiol in vitro", volume = "33", number = "2", pages = "148-151", doi = "10.1016/S0188-4409(01)00355-1" }
Đelić, N.,& Đelić, D.. (2002). Enhanced sister-chromatid exchange rate in human lymphocytes exposed to 17 beta estradiol in vitro. in Archives of Medical Research Elsevier Science Inc, New York., 33(2), 148-151. https://doi.org/10.1016/S0188-4409(01)00355-1
Đelić N, Đelić D. Enhanced sister-chromatid exchange rate in human lymphocytes exposed to 17 beta estradiol in vitro. in Archives of Medical Research. 2002;33(2):148-151. doi:10.1016/S0188-4409(01)00355-1 .
Đelić, Ninoslav, Đelić, D, "Enhanced sister-chromatid exchange rate in human lymphocytes exposed to 17 beta estradiol in vitro" in Archives of Medical Research, 33, no. 2 (2002):148-151, https://doi.org/10.1016/S0188-4409(01)00355-1 . .