Antidepressant effects of an inverse agonist selective for α5 GABA-A receptors in the rat forced swim test
Antidepresivni efekti inverznog agoniste selektivnog za α5 gaba-a receptore u testu forsiranog plivanja pacova
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
It has been shown in electrophysiological studies that the ligand L-655,708 possesses a binding selectivity and a moderate inverse agonist functional selectivity for α5-containing GABA-A receptors. The present study is aimed to investigate the antidepressant effects of the ligand L-655,708 in the forced swim test (FST) and its impact on locomotor activity in rats. The behavior of the animals was recorded with a digital camera, and the data were analyzed by one-way ANOVA, followed by Dunnett’s test. In FST, L-655,708 significantly decreased immobility time at a dose of 3 mg/kg after a single and repeated administration (p<0.05), exerting acute and chronic antidepressant effects. However, it did not induce significant differences in the time of struggling behavior during FST. Furthermore, L-655,708 did not show a significant effect on locomotor activity (p>0.05). These data suggest that negative modulation at GABA-A receptors containing the α5 subunit may produce antidepressant eff...ects in rats. These effects were not confounded by locomotor influences.
U elektrofi ziološkim ispitivanjima pokazano je da ligand L-655,708 poseduje selektivnost u vezivanju i umerenu inverznu agonističku aktivnost za GABA-A receptore koji sadrže α5 podjedinicu. Ova studija ima za cilj ispitivanje antidepresivnih efekata liganda L-655,708 u testu forsiranog plivanja (FST) i njegov uticaj na lokomotornu aktivnost kod pacova. Ponašanje životinja je praćeno pomoću digitalne kamere, a podaci su analizirani primenom jednostepene analize varijanse (ANOVA) i Dunett-ovim testom. U testu forsiranog plivanja L-655,708 statistički značajno smanjuje vreme imobilnosti pri dozi od 3 mg/kg, nakon jednokratne i ponavljane primene (p0,05). Ovi podaci ukazuju da negativna modulacija GABA-A receptora koji sadrže α5 podjedinicu može ispoljiti antidepresivne efekte kod pacova. Ovi efekti su bez izraženih lokomotornih uticaja.
Кључне речи:
GABA-A receptor / Inverse agonist / Depression / Forced swim test / Locomotor activityИзвор:
Acta Veterinaria-Beograd, 2014, 64, 1, 52-60Финансирање / пројекти:
- Бихејвиорални ефекти понављане примене новосинтетисаних супстанци селективних за поједине подтипове бензодиазепинског места везивања ГАБА А рецептора: поређење са стандардним психофармаколошким лековима (RS-MESTD-Basic Research (BR or ON)-175076)
DOI: 10.2478/acve-2014-0006
ISSN: 0567-8315
WoS: 000341341000006
Scopus: 2-s2.0-84902506970
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Samardžić, Janko AU - Puškaš, Laslo AU - Obradović, Miljana AU - Lazić-Puškaš, Dijana PY - 2014 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2187 AB - It has been shown in electrophysiological studies that the ligand L-655,708 possesses a binding selectivity and a moderate inverse agonist functional selectivity for α5-containing GABA-A receptors. The present study is aimed to investigate the antidepressant effects of the ligand L-655,708 in the forced swim test (FST) and its impact on locomotor activity in rats. The behavior of the animals was recorded with a digital camera, and the data were analyzed by one-way ANOVA, followed by Dunnett’s test. In FST, L-655,708 significantly decreased immobility time at a dose of 3 mg/kg after a single and repeated administration (p<0.05), exerting acute and chronic antidepressant effects. However, it did not induce significant differences in the time of struggling behavior during FST. Furthermore, L-655,708 did not show a significant effect on locomotor activity (p>0.05). These data suggest that negative modulation at GABA-A receptors containing the α5 subunit may produce antidepressant effects in rats. These effects were not confounded by locomotor influences. AB - U elektrofi ziološkim ispitivanjima pokazano je da ligand L-655,708 poseduje selektivnost u vezivanju i umerenu inverznu agonističku aktivnost za GABA-A receptore koji sadrže α5 podjedinicu. Ova studija ima za cilj ispitivanje antidepresivnih efekata liganda L-655,708 u testu forsiranog plivanja (FST) i njegov uticaj na lokomotornu aktivnost kod pacova. Ponašanje životinja je praćeno pomoću digitalne kamere, a podaci su analizirani primenom jednostepene analize varijanse (ANOVA) i Dunett-ovim testom. U testu forsiranog plivanja L-655,708 statistički značajno smanjuje vreme imobilnosti pri dozi od 3 mg/kg, nakon jednokratne i ponavljane primene (p0,05). Ovi podaci ukazuju da negativna modulacija GABA-A receptora koji sadrže α5 podjedinicu može ispoljiti antidepresivne efekte kod pacova. Ovi efekti su bez izraženih lokomotornih uticaja. T2 - Acta Veterinaria-Beograd T1 - Antidepressant effects of an inverse agonist selective for α5 GABA-A receptors in the rat forced swim test T1 - Antidepresivni efekti inverznog agoniste selektivnog za α5 gaba-a receptore u testu forsiranog plivanja pacova VL - 64 IS - 1 SP - 52 EP - 60 DO - 10.2478/acve-2014-0006 ER -
@article{ author = "Samardžić, Janko and Puškaš, Laslo and Obradović, Miljana and Lazić-Puškaš, Dijana", year = "2014", abstract = "It has been shown in electrophysiological studies that the ligand L-655,708 possesses a binding selectivity and a moderate inverse agonist functional selectivity for α5-containing GABA-A receptors. The present study is aimed to investigate the antidepressant effects of the ligand L-655,708 in the forced swim test (FST) and its impact on locomotor activity in rats. The behavior of the animals was recorded with a digital camera, and the data were analyzed by one-way ANOVA, followed by Dunnett’s test. In FST, L-655,708 significantly decreased immobility time at a dose of 3 mg/kg after a single and repeated administration (p<0.05), exerting acute and chronic antidepressant effects. However, it did not induce significant differences in the time of struggling behavior during FST. Furthermore, L-655,708 did not show a significant effect on locomotor activity (p>0.05). These data suggest that negative modulation at GABA-A receptors containing the α5 subunit may produce antidepressant effects in rats. These effects were not confounded by locomotor influences., U elektrofi ziološkim ispitivanjima pokazano je da ligand L-655,708 poseduje selektivnost u vezivanju i umerenu inverznu agonističku aktivnost za GABA-A receptore koji sadrže α5 podjedinicu. Ova studija ima za cilj ispitivanje antidepresivnih efekata liganda L-655,708 u testu forsiranog plivanja (FST) i njegov uticaj na lokomotornu aktivnost kod pacova. Ponašanje životinja je praćeno pomoću digitalne kamere, a podaci su analizirani primenom jednostepene analize varijanse (ANOVA) i Dunett-ovim testom. U testu forsiranog plivanja L-655,708 statistički značajno smanjuje vreme imobilnosti pri dozi od 3 mg/kg, nakon jednokratne i ponavljane primene (p0,05). Ovi podaci ukazuju da negativna modulacija GABA-A receptora koji sadrže α5 podjedinicu može ispoljiti antidepresivne efekte kod pacova. Ovi efekti su bez izraženih lokomotornih uticaja.", journal = "Acta Veterinaria-Beograd", title = "Antidepressant effects of an inverse agonist selective for α5 GABA-A receptors in the rat forced swim test, Antidepresivni efekti inverznog agoniste selektivnog za α5 gaba-a receptore u testu forsiranog plivanja pacova", volume = "64", number = "1", pages = "52-60", doi = "10.2478/acve-2014-0006" }
Samardžić, J., Puškaš, L., Obradović, M.,& Lazić-Puškaš, D.. (2014). Antidepressant effects of an inverse agonist selective for α5 GABA-A receptors in the rat forced swim test. in Acta Veterinaria-Beograd, 64(1), 52-60. https://doi.org/10.2478/acve-2014-0006
Samardžić J, Puškaš L, Obradović M, Lazić-Puškaš D. Antidepressant effects of an inverse agonist selective for α5 GABA-A receptors in the rat forced swim test. in Acta Veterinaria-Beograd. 2014;64(1):52-60. doi:10.2478/acve-2014-0006 .
Samardžić, Janko, Puškaš, Laslo, Obradović, Miljana, Lazić-Puškaš, Dijana, "Antidepressant effects of an inverse agonist selective for α5 GABA-A receptors in the rat forced swim test" in Acta Veterinaria-Beograd, 64, no. 1 (2014):52-60, https://doi.org/10.2478/acve-2014-0006 . .