A single dose vaccination with an elastase-dependent H1N1 live attenuated swine influenza virus protects pigs from challenge with 2009 pandemic H1N1 virus

Abstract

The 2009 outbreak of H1N1 influenza A viruses in humans underscored the importance of pigs in influenza A virus evolution and the emergence of novel viruses with pandemic potential. In addition, influenza A virus infections continued to cause production losses in the agricultural industry resulting in a significant drop of profit. The primary method to control influenza A virus infections in pigs is through vaccination. Previously we demonstrated that two doses of an elastase-dependent live attenuated swine influenza virus administered by either the intratracheal or intranasal route can provide a high degree of protection in pigs against challenge with both homologous and different heterologous swine influenza viruses. Here we report the protection efficacy of a single dose elastase-dependent live attenuated swine influenza virus administered by the intranasal route against challenge with homologous subtypic H1N1 2009 pandemic swine-like influenza virus. Protection was observed in the absence of neutralizing antibodies specific for H1N1 2009 in sera.

Pandemija izazvana virusom influence A tipa H1N1 u 2009. godini, istakla je značaj svinja u evoluciji virusa infl uence A i pojavu novih virusa sa pandemijskim potencijalom. Takođe, infekcije virusom infl uence A značajno doprinose smanjenju proizvodnje u poljoprivrednoj industriji što za rezultat ima povećanje gubitaka i značajan pad profi ta. Primarni metod za kontrolu virusnih infekcija izazvanih virusom infl uence A kod svinja je putem vakcinacije. Prethodno smo demonstrirali da dve doze elastaza-zavisnog, živog atenuiranog virusa infl uence svinja date intratrahealno ili intranazalno mogu da obezbede visok stepen zaštite svinja od novih infekcija sa homolognim i različitim heterologim virusima infl uence svinja. U ovom radu smo opisali efi kasnost vakcinacije jedne doze elastaza-zavisnog, živog atenuiranog virusa infl uence svinja, date intranazalno, protiv infekcije sa homolognim 2009 pandemičnim virusom subtipa H1N1. Zaštita protiv H1N1 2009 virusa kod vakcinisanih svinja je primećena i u odsustvu neutralizacionih antitela u serumu specifičnih za H1N1 2009 virus.