Sarcoid in the lower eyelid due to bovine papillomavirus-2 in a donkey (Equus asinus)

Abstract

Sarcoid tumors were described by means of histopathological and molecular procedures in a 5-year-old donkey. Histopathological examination showed epithelial changes including hyperkeratosis, epithelial hyperplasia, koilocytosis, and rete peg formation. Neoplastic fibroblastic cells were plumb, large spindle to stellate and embedded in dense collagenous tissue. Results of Polymerase Chain Reaction and DNA sequence analysis showed that the etiological agent belonged to Bovine Papilloma Virus-II species in the delta papilloma virus genus. This case study represents the first report demonstrating the presence of Bovine Papilloma Virus-II in donkey sarcoid.

Sarkoidi su opisani primenom histopatoloških i molelularanih metoda i procedura kod magarca, starog pet godina. Histopatološko ispitivanje, ukazalo je na promene u epitelu koje su uključivale hiperkeratozu, hiperplaziju epitela, koilocitozu i formiranje invaginacija epitela ptema dubokom dermisu - rete peg. Fibroblastine neoplastične ćelije su bile krupne, ovalnovretenaste i zvezdaste, uronjene u tkivo bogato kolagenom. Ispitivanje PCR metodom kao i rezultati DNK sekvencioniranja pokazali su da je etiološki agens spadao u grupu bovinog papilomavirusa-2 koji pripada rodu delta papilomavirusa. Ova studija predstavlja prvi opisani slučaj prisustva papiloma virusa goveda-2 kod sarkoida magarca.