Evaluation of Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum
Procena in vitro aktivacije proizvodnje azot oksida kao odgovor bovinih epitelnih ćelija endometrijuma i vagine i mononuklearnih krvnih ćelija na Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum
2021
Аутори
Pôrto, Regiani Nascimento GagnoJunqueira-Kipnis, Ana Paula
Viu, Marco Antonio de Oliveira
Teixeira, Rafaela Cavalcanti
Gambarini, Maria Lucia
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Genital mycoplasmosis is a condition present in bovine production systems, and the most important agents involved are Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum. Some aspects of their pathogenesis remain unclear. This study was designed in order to evaluate their ability to stimulate mononuclear cells from the endometrium, vagina and peripheral blood of cycling and healthy cows to produce nitric oxide (NO). Cellular cultures of endometrial, vaginal and peripheral blood cells from 33 healthy cows were cultivated with Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum originated from the 4th passage in culture broth and the NO production was measured by the Greiss reaction. Confi rmation of the presence of mononuclear cells and of the agents during and after the NO assay was done by Giemsa stained smears and further cultivation and detection by PCR reaction. Mononuclear cells from all samples produced NO. Mycoplasma bovigenitalium stimulated high...er NO production than the others (p<0.05). Endometrial cells produced less NO than vaginal or blood cultured cells. In conclusion, it seems that Mycoplasma bovis, Mycoplasma bovigenitalium, and Ureaplasma diversum are able to activate mononuclear cells and induce the production of NO, thus suggesting that this pathway is elicited in response to the primary infection by these agents. More studies are necessary to verify why these agents remain in the bovine reproductive tract for long periods and how they reassume deleterious effects.
Genitalna mikoplazmoza je stanje koje može biti prisutno u reproduktivnom sistemu goveda, a najznačajniji agensi koji je izazivaju su Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum. Neki od aspekata patogeneze nisu još uvek u potpunosti razjašnjeni. Ova studija je kreirana kako bi se procenila njihova sposobnost stimulisanja mononuklearnih ć elija iz endometrijuma, vagine i periferne krvi krava u ciklusu i zdravih krava da proizvode azotni oksid (NO). Ć elijske kulture ć elija endometrijuma, vagine i periferne krvi 33 zdrave krave uzgajane su sa Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum proisteklim iz 4 pasaže u bujonu, a proizvodnja NO merena je Greiss reakcijom. Potvrda prisustva mononuklearnih ć elija, kao i infektivnih agenasa tokom i posle određivanja NO, urađena je Giemsa bojenjem na razmazima, kao i daljom kultivacijom i detekcijom PCR reakcijom. Mononuklearne ć elije iz svih uzoraka proizvodile su NO. Mycoplasma bovigenitalium stimulis...ala je već u proizvodnju NO od ostalih (p
Кључне речи:
cow / Genital micoplasmosis / innate immunity / infectionИзвор:
Acta Veterinaria-Beograd, 2021, 71, 2, 137-146Финансирање / пројекти:
- This project was supported by the National Council for Scientifi c and Technological Development (CNPq), Brazil, (Process #448904/2014-4)
- Research grant to MLGambarini (Process #310581/2016-8).
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Pôrto, Regiani Nascimento Gagno AU - Junqueira-Kipnis, Ana Paula AU - Viu, Marco Antonio de Oliveira AU - Teixeira, Rafaela Cavalcanti AU - Gambarini, Maria Lucia PY - 2021 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2208 AB - Genital mycoplasmosis is a condition present in bovine production systems, and the most important agents involved are Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum. Some aspects of their pathogenesis remain unclear. This study was designed in order to evaluate their ability to stimulate mononuclear cells from the endometrium, vagina and peripheral blood of cycling and healthy cows to produce nitric oxide (NO). Cellular cultures of endometrial, vaginal and peripheral blood cells from 33 healthy cows were cultivated with Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum originated from the 4th passage in culture broth and the NO production was measured by the Greiss reaction. Confi rmation of the presence of mononuclear cells and of the agents during and after the NO assay was done by Giemsa stained smears and further cultivation and detection by PCR reaction. Mononuclear cells from all samples produced NO. Mycoplasma bovigenitalium stimulated higher NO production than the others (p<0.05). Endometrial cells produced less NO than vaginal or blood cultured cells. In conclusion, it seems that Mycoplasma bovis, Mycoplasma bovigenitalium, and Ureaplasma diversum are able to activate mononuclear cells and induce the production of NO, thus suggesting that this pathway is elicited in response to the primary infection by these agents. More studies are necessary to verify why these agents remain in the bovine reproductive tract for long periods and how they reassume deleterious effects. AB - Genitalna mikoplazmoza je stanje koje može biti prisutno u reproduktivnom sistemu goveda, a najznačajniji agensi koji je izazivaju su Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum. Neki od aspekata patogeneze nisu još uvek u potpunosti razjašnjeni. Ova studija je kreirana kako bi se procenila njihova sposobnost stimulisanja mononuklearnih ć elija iz endometrijuma, vagine i periferne krvi krava u ciklusu i zdravih krava da proizvode azotni oksid (NO). Ć elijske kulture ć elija endometrijuma, vagine i periferne krvi 33 zdrave krave uzgajane su sa Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum proisteklim iz 4 pasaže u bujonu, a proizvodnja NO merena je Greiss reakcijom. Potvrda prisustva mononuklearnih ć elija, kao i infektivnih agenasa tokom i posle određivanja NO, urađena je Giemsa bojenjem na razmazima, kao i daljom kultivacijom i detekcijom PCR reakcijom. Mononuklearne ć elije iz svih uzoraka proizvodile su NO. Mycoplasma bovigenitalium stimulisala je već u proizvodnju NO od ostalih (p T2 - Acta Veterinaria-Beograd T1 - Evaluation of Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum T1 - Procena in vitro aktivacije proizvodnje azot oksida kao odgovor bovinih epitelnih ćelija endometrijuma i vagine i mononuklearnih krvnih ćelija na Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum VL - 71 IS - 2 SP - 137 EP - 146 DO - 10.2478/acve-2021-0012 ER -
@article{ author = "Pôrto, Regiani Nascimento Gagno and Junqueira-Kipnis, Ana Paula and Viu, Marco Antonio de Oliveira and Teixeira, Rafaela Cavalcanti and Gambarini, Maria Lucia", year = "2021", abstract = "Genital mycoplasmosis is a condition present in bovine production systems, and the most important agents involved are Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum. Some aspects of their pathogenesis remain unclear. This study was designed in order to evaluate their ability to stimulate mononuclear cells from the endometrium, vagina and peripheral blood of cycling and healthy cows to produce nitric oxide (NO). Cellular cultures of endometrial, vaginal and peripheral blood cells from 33 healthy cows were cultivated with Mycoplasma bovis, Mycoplasma bovigenitalium and Ureaplasma diversum originated from the 4th passage in culture broth and the NO production was measured by the Greiss reaction. Confi rmation of the presence of mononuclear cells and of the agents during and after the NO assay was done by Giemsa stained smears and further cultivation and detection by PCR reaction. Mononuclear cells from all samples produced NO. Mycoplasma bovigenitalium stimulated higher NO production than the others (p<0.05). Endometrial cells produced less NO than vaginal or blood cultured cells. In conclusion, it seems that Mycoplasma bovis, Mycoplasma bovigenitalium, and Ureaplasma diversum are able to activate mononuclear cells and induce the production of NO, thus suggesting that this pathway is elicited in response to the primary infection by these agents. More studies are necessary to verify why these agents remain in the bovine reproductive tract for long periods and how they reassume deleterious effects., Genitalna mikoplazmoza je stanje koje može biti prisutno u reproduktivnom sistemu goveda, a najznačajniji agensi koji je izazivaju su Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum. Neki od aspekata patogeneze nisu još uvek u potpunosti razjašnjeni. Ova studija je kreirana kako bi se procenila njihova sposobnost stimulisanja mononuklearnih ć elija iz endometrijuma, vagine i periferne krvi krava u ciklusu i zdravih krava da proizvode azotni oksid (NO). Ć elijske kulture ć elija endometrijuma, vagine i periferne krvi 33 zdrave krave uzgajane su sa Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum proisteklim iz 4 pasaže u bujonu, a proizvodnja NO merena je Greiss reakcijom. Potvrda prisustva mononuklearnih ć elija, kao i infektivnih agenasa tokom i posle određivanja NO, urađena je Giemsa bojenjem na razmazima, kao i daljom kultivacijom i detekcijom PCR reakcijom. Mononuklearne ć elije iz svih uzoraka proizvodile su NO. Mycoplasma bovigenitalium stimulisala je već u proizvodnju NO od ostalih (p", journal = "Acta Veterinaria-Beograd", title = "Evaluation of Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum, Procena in vitro aktivacije proizvodnje azot oksida kao odgovor bovinih epitelnih ćelija endometrijuma i vagine i mononuklearnih krvnih ćelija na Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum", volume = "71", number = "2", pages = "137-146", doi = "10.2478/acve-2021-0012" }
Pôrto, R. N. G., Junqueira-Kipnis, A. P., Viu, M. A. d. O., Teixeira, R. C.,& Gambarini, M. L.. (2021). Evaluation of Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum. in Acta Veterinaria-Beograd, 71(2), 137-146. https://doi.org/10.2478/acve-2021-0012
Pôrto RNG, Junqueira-Kipnis AP, Viu MADO, Teixeira RC, Gambarini ML. Evaluation of Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum. in Acta Veterinaria-Beograd. 2021;71(2):137-146. doi:10.2478/acve-2021-0012 .
Pôrto, Regiani Nascimento Gagno, Junqueira-Kipnis, Ana Paula, Viu, Marco Antonio de Oliveira, Teixeira, Rafaela Cavalcanti, Gambarini, Maria Lucia, "Evaluation of Activation of Bovine Endometrial and Vaginal Epithelial and Blood Mononuclear Cells to Produce Nitric Oxide in Response to Mycoplasma bovis, Mycoplasma bovigenitalium i Ureaplasma diversum" in Acta Veterinaria-Beograd, 71, no. 2 (2021):137-146, https://doi.org/10.2478/acve-2021-0012 . .