Evaluation of Thymol-β-d-Glucopyranoside as a Potential Prebiotic Intervention to Reduce Carriage of Zoonotic Pathogens in Weaned and Feeder Pigs
2021
Аутори
Levent, GizemAnderson, Robin C.
Petrujkić, Branko
Poole, Toni L.
He, Haiqi
Genovese, Kenneth J.
Hume, Michael E.
Beier, Ross C.
Harvey, Roger B.
Nisbet, David J.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The gut of food-producing animals is a reservoir for foodborne pathogens. Thymol is bactericidal against foodborne pathogens but rapid absorption of thymol from the proximal gut precludes the delivery of effective concentrations to the lower gut where pathogens mainly colonize. Thymol-β-d-glucopyranoside is reported to be more resistant to absorption than thymol in everted jejunal segments and could potentially function as a prebiotic by resisting degradation and absorption in the proximal gut but being hydrolysable by microbial β-glycosidase in the distal gut. Previous in vitro studies showed bactericidal effects of thymol-β-d-glucopyranoside against Campylobacter, Escherichia coli, and Salmonella enterica serovar Typhimurium in the presence but not absence of intestinal microbes expressing β-glycosidase activity, indicating that hydrolysis was required to obtain antimicrobial activity. Presently, the oral administration of thymol-β-d-glucopyranoside was studied to examine the effects... on intestinal carriage of Campylobacter, E. coli, and S. Typhimurium in swine. The effects of thymol-β-d-glucopyranoside or thymol on antimicrobial sensitivity of representative E. coli isolates and characterized Salmonella strains were also explored. Results from two in vivo studies revealed little antimicrobial effects of thymol-β-d-glucopyranoside on Campylobacter, E. coli, or S. Typhimurium in swine gut. These findings add credence to current thinking that hydrolysis and absorption of thymol-β-d-glucopyranoside and thymol may be sufficiently rapid within the proximal gut to preclude delivery to the distal gut. Antibiotic susceptibilities of selected bacterial isolates and strains were mainly unaffected by thymol. Further research is warranted to overcome obstacles, preventing the delivery of efficacious amounts of thymol-β-d-glucopyranoside to the lower gut.
Кључне речи:
swine / Campylobacter / Escherichia coli / Salmonella / thymol / thymol-β-d-glucopyranosideИзвор:
Microorganisms, 2021, 9, 4, 860-Издавач:
- MDPI
Финансирање / пројекти:
- National Pork Board Grant 14-077
- Research funds appropriated by the United States Department of Agriculture
DOI: 10.3390/microorganisms9040860
ISSN: 2076-2607
WoS: 000643309900001
Scopus: 2-s2.0-85104567028
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Levent, Gizem AU - Anderson, Robin C. AU - Petrujkić, Branko AU - Poole, Toni L. AU - He, Haiqi AU - Genovese, Kenneth J. AU - Hume, Michael E. AU - Beier, Ross C. AU - Harvey, Roger B. AU - Nisbet, David J. PY - 2021 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2221 AB - The gut of food-producing animals is a reservoir for foodborne pathogens. Thymol is bactericidal against foodborne pathogens but rapid absorption of thymol from the proximal gut precludes the delivery of effective concentrations to the lower gut where pathogens mainly colonize. Thymol-β-d-glucopyranoside is reported to be more resistant to absorption than thymol in everted jejunal segments and could potentially function as a prebiotic by resisting degradation and absorption in the proximal gut but being hydrolysable by microbial β-glycosidase in the distal gut. Previous in vitro studies showed bactericidal effects of thymol-β-d-glucopyranoside against Campylobacter, Escherichia coli, and Salmonella enterica serovar Typhimurium in the presence but not absence of intestinal microbes expressing β-glycosidase activity, indicating that hydrolysis was required to obtain antimicrobial activity. Presently, the oral administration of thymol-β-d-glucopyranoside was studied to examine the effects on intestinal carriage of Campylobacter, E. coli, and S. Typhimurium in swine. The effects of thymol-β-d-glucopyranoside or thymol on antimicrobial sensitivity of representative E. coli isolates and characterized Salmonella strains were also explored. Results from two in vivo studies revealed little antimicrobial effects of thymol-β-d-glucopyranoside on Campylobacter, E. coli, or S. Typhimurium in swine gut. These findings add credence to current thinking that hydrolysis and absorption of thymol-β-d-glucopyranoside and thymol may be sufficiently rapid within the proximal gut to preclude delivery to the distal gut. Antibiotic susceptibilities of selected bacterial isolates and strains were mainly unaffected by thymol. Further research is warranted to overcome obstacles, preventing the delivery of efficacious amounts of thymol-β-d-glucopyranoside to the lower gut. PB - MDPI T2 - Microorganisms T1 - Evaluation of Thymol-β-d-Glucopyranoside as a Potential Prebiotic Intervention to Reduce Carriage of Zoonotic Pathogens in Weaned and Feeder Pigs VL - 9 IS - 4 SP - 860 DO - 10.3390/microorganisms9040860 ER -
@article{ author = "Levent, Gizem and Anderson, Robin C. and Petrujkić, Branko and Poole, Toni L. and He, Haiqi and Genovese, Kenneth J. and Hume, Michael E. and Beier, Ross C. and Harvey, Roger B. and Nisbet, David J.", year = "2021", abstract = "The gut of food-producing animals is a reservoir for foodborne pathogens. Thymol is bactericidal against foodborne pathogens but rapid absorption of thymol from the proximal gut precludes the delivery of effective concentrations to the lower gut where pathogens mainly colonize. Thymol-β-d-glucopyranoside is reported to be more resistant to absorption than thymol in everted jejunal segments and could potentially function as a prebiotic by resisting degradation and absorption in the proximal gut but being hydrolysable by microbial β-glycosidase in the distal gut. Previous in vitro studies showed bactericidal effects of thymol-β-d-glucopyranoside against Campylobacter, Escherichia coli, and Salmonella enterica serovar Typhimurium in the presence but not absence of intestinal microbes expressing β-glycosidase activity, indicating that hydrolysis was required to obtain antimicrobial activity. Presently, the oral administration of thymol-β-d-glucopyranoside was studied to examine the effects on intestinal carriage of Campylobacter, E. coli, and S. Typhimurium in swine. The effects of thymol-β-d-glucopyranoside or thymol on antimicrobial sensitivity of representative E. coli isolates and characterized Salmonella strains were also explored. Results from two in vivo studies revealed little antimicrobial effects of thymol-β-d-glucopyranoside on Campylobacter, E. coli, or S. Typhimurium in swine gut. These findings add credence to current thinking that hydrolysis and absorption of thymol-β-d-glucopyranoside and thymol may be sufficiently rapid within the proximal gut to preclude delivery to the distal gut. Antibiotic susceptibilities of selected bacterial isolates and strains were mainly unaffected by thymol. Further research is warranted to overcome obstacles, preventing the delivery of efficacious amounts of thymol-β-d-glucopyranoside to the lower gut.", publisher = "MDPI", journal = "Microorganisms", title = "Evaluation of Thymol-β-d-Glucopyranoside as a Potential Prebiotic Intervention to Reduce Carriage of Zoonotic Pathogens in Weaned and Feeder Pigs", volume = "9", number = "4", pages = "860", doi = "10.3390/microorganisms9040860" }
Levent, G., Anderson, R. C., Petrujkić, B., Poole, T. L., He, H., Genovese, K. J., Hume, M. E., Beier, R. C., Harvey, R. B.,& Nisbet, D. J.. (2021). Evaluation of Thymol-β-d-Glucopyranoside as a Potential Prebiotic Intervention to Reduce Carriage of Zoonotic Pathogens in Weaned and Feeder Pigs. in Microorganisms MDPI., 9(4), 860. https://doi.org/10.3390/microorganisms9040860
Levent G, Anderson RC, Petrujkić B, Poole TL, He H, Genovese KJ, Hume ME, Beier RC, Harvey RB, Nisbet DJ. Evaluation of Thymol-β-d-Glucopyranoside as a Potential Prebiotic Intervention to Reduce Carriage of Zoonotic Pathogens in Weaned and Feeder Pigs. in Microorganisms. 2021;9(4):860. doi:10.3390/microorganisms9040860 .
Levent, Gizem, Anderson, Robin C., Petrujkić, Branko, Poole, Toni L., He, Haiqi, Genovese, Kenneth J., Hume, Michael E., Beier, Ross C., Harvey, Roger B., Nisbet, David J., "Evaluation of Thymol-β-d-Glucopyranoside as a Potential Prebiotic Intervention to Reduce Carriage of Zoonotic Pathogens in Weaned and Feeder Pigs" in Microorganisms, 9, no. 4 (2021):860, https://doi.org/10.3390/microorganisms9040860 . .