Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model
Procena regenerativnih procesa mekih tkiva nakon supkutane implantacije srebro / poli (vinil alkohol) i novog srebro / poli (vinil alkohol) / grafenskog hidrogela na animalnom modelu
2021
Аутори
Lužajić Božinovski, TijanaTodorović, Vera
Milošević, Ivan
Gajdov, Vladimir
Prokić, Bogomir Bolka
Nešović, Katarina
Mišković-Stanković, Vesna
Marković, Danica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials – silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle exp...ression actin in blood vessels (α-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue
Bilo koji novoproizvedeni biomaterijal nužno podleže standardima, po čijim se protokolima sprovode in vivo istraživanja na životinjskim modelima. Za procenu mogućnosti regeneracije mekog tkiva nakon potkožne implantacije biomaterijala - srebra/poli(vinil alkohola) (Ag/PVA) i novog materijala srebra/poli(vinil alkohola)/grafena (Ag/PVA/ Gr) predviđenog za kliničku upotrebu, šesnaest pacova je korišćeno, po uputstvima međunarodnog standarda, ISO 10993-6 2007. Histološki uzorci su sakupljeni 7, 15, 30 i 60 dana nakon implantacije. Ovi hidrogelovi su proizvedeni in situ elektrohemijskom sintezom nanočestica srebra u polimernim matricama, što je omogućilo dobijanje potpuno sigurnih i biokompatibilnih materijala, bez ikakvih dodatnih toksičnih hemijskih sredstava za redukciju. Hirurška implantacija hidrogela urađena je prema dozvoli Etičkog komiteta Fakulteta veterinarske medicine Univerziteta u Beogradu. Imunohistohemijske (IHC) studije uključivale su procenu ekspresije aktina glatkih mišić...a u krvnim sudovima (α-SMA), ekspresiju laminina i kolagena tipa I i III u strukturama kože i određivanje ekspresije markera proliferacije (Ki-67) keratinocita. Rezultati su procenjeni semikvantitativnom analizom. Podaci su analizirani u statističkom softverskom paketu IBM SPSS 20. Zaključci su ukazali da se Ag/PVA/Gr može koristiti kao obloga za rane kako bi se povećao potencijal zarastanja tkiva i uspostavila brža integracija i kraće zadržavanje u tkivu.
Кључне речи:
immunohistochemistry / nanocomposite biomaterials / rat / skin regenerationИзвор:
Acta Veterinaria-Beograd, 2021, 71, 3, 285-302Издавач:
- Faculty of Veterinary Medicine, University of Belgrade
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
- European Commisson, project “Twinning to excel materials engineering for medical devices – ExcellMater“ grant no. 952033, H2020-WIDESPREAD-2018-2020/H2020-WIDESPREAD-2020-5, 2020- 2023.3
DOI: 10.2478/acve-2021-0025
ISSN: 0567-8315
WoS: 000703661100004
Scopus: 2-s2.0-85116478653
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Lužajić Božinovski, Tijana AU - Todorović, Vera AU - Milošević, Ivan AU - Gajdov, Vladimir AU - Prokić, Bogomir Bolka AU - Nešović, Katarina AU - Mišković-Stanković, Vesna AU - Marković, Danica PY - 2021 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2241 AB - A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials – silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expression actin in blood vessels (α-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue AB - Bilo koji novoproizvedeni biomaterijal nužno podleže standardima, po čijim se protokolima sprovode in vivo istraživanja na životinjskim modelima. Za procenu mogućnosti regeneracije mekog tkiva nakon potkožne implantacije biomaterijala - srebra/poli(vinil alkohola) (Ag/PVA) i novog materijala srebra/poli(vinil alkohola)/grafena (Ag/PVA/ Gr) predviđenog za kliničku upotrebu, šesnaest pacova je korišćeno, po uputstvima međunarodnog standarda, ISO 10993-6 2007. Histološki uzorci su sakupljeni 7, 15, 30 i 60 dana nakon implantacije. Ovi hidrogelovi su proizvedeni in situ elektrohemijskom sintezom nanočestica srebra u polimernim matricama, što je omogućilo dobijanje potpuno sigurnih i biokompatibilnih materijala, bez ikakvih dodatnih toksičnih hemijskih sredstava za redukciju. Hirurška implantacija hidrogela urađena je prema dozvoli Etičkog komiteta Fakulteta veterinarske medicine Univerziteta u Beogradu. Imunohistohemijske (IHC) studije uključivale su procenu ekspresije aktina glatkih mišića u krvnim sudovima (α-SMA), ekspresiju laminina i kolagena tipa I i III u strukturama kože i određivanje ekspresije markera proliferacije (Ki-67) keratinocita. Rezultati su procenjeni semikvantitativnom analizom. Podaci su analizirani u statističkom softverskom paketu IBM SPSS 20. Zaključci su ukazali da se Ag/PVA/Gr može koristiti kao obloga za rane kako bi se povećao potencijal zarastanja tkiva i uspostavila brža integracija i kraće zadržavanje u tkivu. PB - Faculty of Veterinary Medicine, University of Belgrade T2 - Acta Veterinaria-Beograd T1 - Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model T1 - Procena regenerativnih procesa mekih tkiva nakon supkutane implantacije srebro / poli (vinil alkohol) i novog srebro / poli (vinil alkohol) / grafenskog hidrogela na animalnom modelu VL - 71 IS - 3 SP - 285 EP - 302 DO - 10.2478/acve-2021-0025 ER -
@article{ author = "Lužajić Božinovski, Tijana and Todorović, Vera and Milošević, Ivan and Gajdov, Vladimir and Prokić, Bogomir Bolka and Nešović, Katarina and Mišković-Stanković, Vesna and Marković, Danica", year = "2021", abstract = "A newly produced biomaterial is necessarily subject of standards, which are performed in vivo on animal models. For the evaluation of soft tissue regenerative possibilities after subcutaneous implantation of biomaterials – silver/poly(vinyl alcohol) (Ag/PVA) and novel silver/poly(vinyl alcohol)/graphene (Ag/PVA/Gr) provided for clinical use, sixteen rats were used, according to the instructions of international standards, ISO 10993-6, 2007. Histological sections were observed 7, 15, 30 and 60 days after grafting. These hydrogels were produced by in situ electrochemical synthesis of silver nanoparticles in the polymer matrices, which enabled obtaining completely safe and biocompatible materials, free from any additional toxic chemical reducing agents. Surgical implantation of hydrogels was done according to the permission of the Ethical Committee of the Faculty of Veterinary Medicine, University of Belgrade. Immunohistochemical (IHC) studies included the assessment of smooth muscle expression actin in blood vessels (α-SMA), the expression of laminin and type I and type III collagen in the skin structures, and, the determination of cell proliferation marker expression (Ki-67) keratinocytes. The results were assessed in a semiquantitative manner. The data were analyzed in the statistical software package IBM SPSS 20. The conclusions indicated that Ag/PVA/Gr might be used as wound dressings to enhance the tissue, Bilo koji novoproizvedeni biomaterijal nužno podleže standardima, po čijim se protokolima sprovode in vivo istraživanja na životinjskim modelima. Za procenu mogućnosti regeneracije mekog tkiva nakon potkožne implantacije biomaterijala - srebra/poli(vinil alkohola) (Ag/PVA) i novog materijala srebra/poli(vinil alkohola)/grafena (Ag/PVA/ Gr) predviđenog za kliničku upotrebu, šesnaest pacova je korišćeno, po uputstvima međunarodnog standarda, ISO 10993-6 2007. Histološki uzorci su sakupljeni 7, 15, 30 i 60 dana nakon implantacije. Ovi hidrogelovi su proizvedeni in situ elektrohemijskom sintezom nanočestica srebra u polimernim matricama, što je omogućilo dobijanje potpuno sigurnih i biokompatibilnih materijala, bez ikakvih dodatnih toksičnih hemijskih sredstava za redukciju. Hirurška implantacija hidrogela urađena je prema dozvoli Etičkog komiteta Fakulteta veterinarske medicine Univerziteta u Beogradu. Imunohistohemijske (IHC) studije uključivale su procenu ekspresije aktina glatkih mišića u krvnim sudovima (α-SMA), ekspresiju laminina i kolagena tipa I i III u strukturama kože i određivanje ekspresije markera proliferacije (Ki-67) keratinocita. Rezultati su procenjeni semikvantitativnom analizom. Podaci su analizirani u statističkom softverskom paketu IBM SPSS 20. Zaključci su ukazali da se Ag/PVA/Gr može koristiti kao obloga za rane kako bi se povećao potencijal zarastanja tkiva i uspostavila brža integracija i kraće zadržavanje u tkivu.", publisher = "Faculty of Veterinary Medicine, University of Belgrade", journal = "Acta Veterinaria-Beograd", title = "Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model, Procena regenerativnih procesa mekih tkiva nakon supkutane implantacije srebro / poli (vinil alkohol) i novog srebro / poli (vinil alkohol) / grafenskog hidrogela na animalnom modelu", volume = "71", number = "3", pages = "285-302", doi = "10.2478/acve-2021-0025" }
Lužajić Božinovski, T., Todorović, V., Milošević, I., Gajdov, V., Prokić, B. B., Nešović, K., Mišković-Stanković, V.,& Marković, D.. (2021). Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model. in Acta Veterinaria-Beograd Faculty of Veterinary Medicine, University of Belgrade., 71(3), 285-302. https://doi.org/10.2478/acve-2021-0025
Lužajić Božinovski T, Todorović V, Milošević I, Gajdov V, Prokić BB, Nešović K, Mišković-Stanković V, Marković D. Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model. in Acta Veterinaria-Beograd. 2021;71(3):285-302. doi:10.2478/acve-2021-0025 .
Lužajić Božinovski, Tijana, Todorović, Vera, Milošević, Ivan, Gajdov, Vladimir, Prokić, Bogomir Bolka, Nešović, Katarina, Mišković-Stanković, Vesna, Marković, Danica, "Evaluation of soft tissue regenerative processes after subcutaneous implantation of silver/poly(vinyl alcohol) and novel silver/poly(vinyl alcohol)/graphene hydrogels in an animal model" in Acta Veterinaria-Beograd, 71, no. 3 (2021):285-302, https://doi.org/10.2478/acve-2021-0025 . .