Functional and histological changes of the pancreas and the liver in the rats after the acute and subacute administration of diazinon
Funkcionalne i histološke promene pankreasa i jetre kod pacova posle akutne i subakutne primene diazinona
2021
Аутори
Ivanović, Saša R.Borozan, Nevena
Janković, Radmila
Ćupić Miladinović, Dejana
Savić, Mila
Ćupić, Vitomir
Borozan, Sunčica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background/Aim. Organophosphate pesticides (OPs) are used extensively worldwide in agriculture and forestry, and their application represents a major health problem for humans and animals. The aim of this study was to investigate the possibility of the adaptation of an organism to the prolonged administration of a low dose of diazinon. Methods. The study was conducted on a total of 60 male Wistar rats. The first 30 rats were divided into four equal diazinon groups (n = 6) and the control one (corn oil). Diazinon was orally administered once at doses: 200, 400, 600, 800 mg/kg (one dose – one group). The concentration of glucose, the activity of α-amylase and the relative activity of LDH1-LDH5 isoenzymes in the blood were measured 24 hours after the application. The remaining 30 rats were divided into two equal diazinon groups (n = 10) and the control one (corn oil). The first group was treated during 7 days, and the second during 14 days with 55 mg/kg of diazinon (1/10 of previously det...ermined LD50 value). The histopathology of the pancreas and the liver, as well as the relative activities of LDH isoenzymes in the blood, were determined after the completion of both time periods. Results. Single administration of increasing doses of diazinon resulted in a significant increase in the concentrations of glucose, activity of α-amylase and LDH isoenzymes. Subacute application of a low diazinon dose induced histopathological changes in the pancreas manifested by acinar cell necrosis, and in the liver in the form of portal hepatitis and multifocal necrosis. The cumulative doses resulted in statistically significantly lower activities of LDH isoenzymes compared with the single administration of these doses, indicating a lower degree of the cells damage after the subacute diazinon administration. Conclusion. Subacute administration of a low dose of diazinon leads to a different adaptation degree of organs and organ systems to toxic effects caused by this organophosphate.
Uvod/Cilj. Organofosfatni pesticidi (OP) se intenzivno koriste širom sveta u poljoprivredi i šumarstvu, a njihova primena predstavlja značajan zdravstveni problem kod ljudi i životinja. Cilj ove studije bio je da se ispita mogućnost adaptacije organizma na prolongiranu primenu niskih doza diazinona. Metode. Studija je sprovedena na ukupno 60 pacova muškog pola Vistar soja. Prvih 30 pacova je podel-jeno u četiri jednake grupe tretirane diazinonom (n = 6) i kontrolnu grupu (kukuruzno ulje). Diazinon je primenjivan jednokratno peroralno u dozama: 200, 400, 600, 800 mg/kg (jedna doza – jedna grupa). Koncentracija glukoze, aktivnost α-amilaze i relativna aktivnost LDH1-LDH5 izoenzima u krvi, određivani su 24 sata nakon aplikacije. Preostalih 30 pacova je podeljeno u dve jednake diazinon grupe (n = 10) i kontrolnu grupu (kukuruzno ulje). Prva grupa je tretirana 7 dana, a druga 14 dana sa 55 mg/kg diazinona (1/10 prethod-no određene vrednosti LD50). Histopatologija pankreasa i jetre i određiv...anje relativne aktivnosti LDH izoenzima u krvi urađeni su po završetku oba vremenska perioda. Rezultati. Jednokratna primena rastućih doza diazinona rezultirala je statistički značajnim povećanjem koncentracije glukoze, ak-tivnosti α-amilaze i LDH izoenzima. Subakutna primena niske doze diazinona indukovala je histopatološke promene u pankreasu manifestovane acinarnom nekrozom, a u jetri promene su se ispoljile u vidu portalnog hepatitisa i multi-fokalne nekroze. Кumulativne doze diazinona rezultirale su statistički značajno nižom aktivnošću LDH izoenzima u poređenju sa jednokratnom primenom tih doza, što ukazuje na niži stepen oštećenja ćelija posle subakutne primene dia-zinona. Zaključak. Subakutna primena niske doze diazinona dovodi do različitog stepena adaptacije organa i organskih sistema na toksične efekte izazvane tim organofosfatom.
Кључне речи:
diazinon / histological techniques / liver / organophosphorus compounds / pancreas / rats / toxicity test / subacute / histološke tehnike / jetra / organofosforna jedinjenja / pankreas / pacovi / toksičnost / subakutna / testoviИзвор:
Vojnosanitetski pregled, 2021, 78, 9, 955-963Издавач:
- Savezno Ministarstvo Odbrane, Beograd
Финансирање / пројекти:
- Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (RS-MESTD-Basic Research (BR or ON)-173034)
- Организовање одрживе производње органског узгоја јагњади као подршка руралног развоја (RS-MESTD-Technological Development (TD or TR)-31085)
DOI: 10.2298/VSP191223012I
ISSN: 0042-8450
WoS: 000707506000009
Scopus: 2-s2.0-85118498199
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Ivanović, Saša R. AU - Borozan, Nevena AU - Janković, Radmila AU - Ćupić Miladinović, Dejana AU - Savić, Mila AU - Ćupić, Vitomir AU - Borozan, Sunčica PY - 2021 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2249 AB - Background/Aim. Organophosphate pesticides (OPs) are used extensively worldwide in agriculture and forestry, and their application represents a major health problem for humans and animals. The aim of this study was to investigate the possibility of the adaptation of an organism to the prolonged administration of a low dose of diazinon. Methods. The study was conducted on a total of 60 male Wistar rats. The first 30 rats were divided into four equal diazinon groups (n = 6) and the control one (corn oil). Diazinon was orally administered once at doses: 200, 400, 600, 800 mg/kg (one dose – one group). The concentration of glucose, the activity of α-amylase and the relative activity of LDH1-LDH5 isoenzymes in the blood were measured 24 hours after the application. The remaining 30 rats were divided into two equal diazinon groups (n = 10) and the control one (corn oil). The first group was treated during 7 days, and the second during 14 days with 55 mg/kg of diazinon (1/10 of previously determined LD50 value). The histopathology of the pancreas and the liver, as well as the relative activities of LDH isoenzymes in the blood, were determined after the completion of both time periods. Results. Single administration of increasing doses of diazinon resulted in a significant increase in the concentrations of glucose, activity of α-amylase and LDH isoenzymes. Subacute application of a low diazinon dose induced histopathological changes in the pancreas manifested by acinar cell necrosis, and in the liver in the form of portal hepatitis and multifocal necrosis. The cumulative doses resulted in statistically significantly lower activities of LDH isoenzymes compared with the single administration of these doses, indicating a lower degree of the cells damage after the subacute diazinon administration. Conclusion. Subacute administration of a low dose of diazinon leads to a different adaptation degree of organs and organ systems to toxic effects caused by this organophosphate. AB - Uvod/Cilj. Organofosfatni pesticidi (OP) se intenzivno koriste širom sveta u poljoprivredi i šumarstvu, a njihova primena predstavlja značajan zdravstveni problem kod ljudi i životinja. Cilj ove studije bio je da se ispita mogućnost adaptacije organizma na prolongiranu primenu niskih doza diazinona. Metode. Studija je sprovedena na ukupno 60 pacova muškog pola Vistar soja. Prvih 30 pacova je podel-jeno u četiri jednake grupe tretirane diazinonom (n = 6) i kontrolnu grupu (kukuruzno ulje). Diazinon je primenjivan jednokratno peroralno u dozama: 200, 400, 600, 800 mg/kg (jedna doza – jedna grupa). Koncentracija glukoze, aktivnost α-amilaze i relativna aktivnost LDH1-LDH5 izoenzima u krvi, određivani su 24 sata nakon aplikacije. Preostalih 30 pacova je podeljeno u dve jednake diazinon grupe (n = 10) i kontrolnu grupu (kukuruzno ulje). Prva grupa je tretirana 7 dana, a druga 14 dana sa 55 mg/kg diazinona (1/10 prethod-no određene vrednosti LD50). Histopatologija pankreasa i jetre i određivanje relativne aktivnosti LDH izoenzima u krvi urađeni su po završetku oba vremenska perioda. Rezultati. Jednokratna primena rastućih doza diazinona rezultirala je statistički značajnim povećanjem koncentracije glukoze, ak-tivnosti α-amilaze i LDH izoenzima. Subakutna primena niske doze diazinona indukovala je histopatološke promene u pankreasu manifestovane acinarnom nekrozom, a u jetri promene su se ispoljile u vidu portalnog hepatitisa i multi-fokalne nekroze. Кumulativne doze diazinona rezultirale su statistički značajno nižom aktivnošću LDH izoenzima u poređenju sa jednokratnom primenom tih doza, što ukazuje na niži stepen oštećenja ćelija posle subakutne primene dia-zinona. Zaključak. Subakutna primena niske doze diazinona dovodi do različitog stepena adaptacije organa i organskih sistema na toksične efekte izazvane tim organofosfatom. PB - Savezno Ministarstvo Odbrane, Beograd T2 - Vojnosanitetski pregled T1 - Functional and histological changes of the pancreas and the liver in the rats after the acute and subacute administration of diazinon T1 - Funkcionalne i histološke promene pankreasa i jetre kod pacova posle akutne i subakutne primene diazinona VL - 78 IS - 9 SP - 955 EP - 963 DO - 10.2298/VSP191223012I ER -
@article{ author = "Ivanović, Saša R. and Borozan, Nevena and Janković, Radmila and Ćupić Miladinović, Dejana and Savić, Mila and Ćupić, Vitomir and Borozan, Sunčica", year = "2021", abstract = "Background/Aim. Organophosphate pesticides (OPs) are used extensively worldwide in agriculture and forestry, and their application represents a major health problem for humans and animals. The aim of this study was to investigate the possibility of the adaptation of an organism to the prolonged administration of a low dose of diazinon. Methods. The study was conducted on a total of 60 male Wistar rats. The first 30 rats were divided into four equal diazinon groups (n = 6) and the control one (corn oil). Diazinon was orally administered once at doses: 200, 400, 600, 800 mg/kg (one dose – one group). The concentration of glucose, the activity of α-amylase and the relative activity of LDH1-LDH5 isoenzymes in the blood were measured 24 hours after the application. The remaining 30 rats were divided into two equal diazinon groups (n = 10) and the control one (corn oil). The first group was treated during 7 days, and the second during 14 days with 55 mg/kg of diazinon (1/10 of previously determined LD50 value). The histopathology of the pancreas and the liver, as well as the relative activities of LDH isoenzymes in the blood, were determined after the completion of both time periods. Results. Single administration of increasing doses of diazinon resulted in a significant increase in the concentrations of glucose, activity of α-amylase and LDH isoenzymes. Subacute application of a low diazinon dose induced histopathological changes in the pancreas manifested by acinar cell necrosis, and in the liver in the form of portal hepatitis and multifocal necrosis. The cumulative doses resulted in statistically significantly lower activities of LDH isoenzymes compared with the single administration of these doses, indicating a lower degree of the cells damage after the subacute diazinon administration. Conclusion. Subacute administration of a low dose of diazinon leads to a different adaptation degree of organs and organ systems to toxic effects caused by this organophosphate., Uvod/Cilj. Organofosfatni pesticidi (OP) se intenzivno koriste širom sveta u poljoprivredi i šumarstvu, a njihova primena predstavlja značajan zdravstveni problem kod ljudi i životinja. Cilj ove studije bio je da se ispita mogućnost adaptacije organizma na prolongiranu primenu niskih doza diazinona. Metode. Studija je sprovedena na ukupno 60 pacova muškog pola Vistar soja. Prvih 30 pacova je podel-jeno u četiri jednake grupe tretirane diazinonom (n = 6) i kontrolnu grupu (kukuruzno ulje). Diazinon je primenjivan jednokratno peroralno u dozama: 200, 400, 600, 800 mg/kg (jedna doza – jedna grupa). Koncentracija glukoze, aktivnost α-amilaze i relativna aktivnost LDH1-LDH5 izoenzima u krvi, određivani su 24 sata nakon aplikacije. Preostalih 30 pacova je podeljeno u dve jednake diazinon grupe (n = 10) i kontrolnu grupu (kukuruzno ulje). Prva grupa je tretirana 7 dana, a druga 14 dana sa 55 mg/kg diazinona (1/10 prethod-no određene vrednosti LD50). Histopatologija pankreasa i jetre i određivanje relativne aktivnosti LDH izoenzima u krvi urađeni su po završetku oba vremenska perioda. Rezultati. Jednokratna primena rastućih doza diazinona rezultirala je statistički značajnim povećanjem koncentracije glukoze, ak-tivnosti α-amilaze i LDH izoenzima. Subakutna primena niske doze diazinona indukovala je histopatološke promene u pankreasu manifestovane acinarnom nekrozom, a u jetri promene su se ispoljile u vidu portalnog hepatitisa i multi-fokalne nekroze. Кumulativne doze diazinona rezultirale su statistički značajno nižom aktivnošću LDH izoenzima u poređenju sa jednokratnom primenom tih doza, što ukazuje na niži stepen oštećenja ćelija posle subakutne primene dia-zinona. Zaključak. Subakutna primena niske doze diazinona dovodi do različitog stepena adaptacije organa i organskih sistema na toksične efekte izazvane tim organofosfatom.", publisher = "Savezno Ministarstvo Odbrane, Beograd", journal = "Vojnosanitetski pregled", title = "Functional and histological changes of the pancreas and the liver in the rats after the acute and subacute administration of diazinon, Funkcionalne i histološke promene pankreasa i jetre kod pacova posle akutne i subakutne primene diazinona", volume = "78", number = "9", pages = "955-963", doi = "10.2298/VSP191223012I" }
Ivanović, S. R., Borozan, N., Janković, R., Ćupić Miladinović, D., Savić, M., Ćupić, V.,& Borozan, S.. (2021). Functional and histological changes of the pancreas and the liver in the rats after the acute and subacute administration of diazinon. in Vojnosanitetski pregled Savezno Ministarstvo Odbrane, Beograd., 78(9), 955-963. https://doi.org/10.2298/VSP191223012I
Ivanović SR, Borozan N, Janković R, Ćupić Miladinović D, Savić M, Ćupić V, Borozan S. Functional and histological changes of the pancreas and the liver in the rats after the acute and subacute administration of diazinon. in Vojnosanitetski pregled. 2021;78(9):955-963. doi:10.2298/VSP191223012I .
Ivanović, Saša R., Borozan, Nevena, Janković, Radmila, Ćupić Miladinović, Dejana, Savić, Mila, Ćupić, Vitomir, Borozan, Sunčica, "Functional and histological changes of the pancreas and the liver in the rats after the acute and subacute administration of diazinon" in Vojnosanitetski pregled, 78, no. 9 (2021):955-963, https://doi.org/10.2298/VSP191223012I . .