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dc.creatorĆupić, Vitomir
dc.creatorColić, M
dc.creatorJandrić, D.
dc.creatorMilojković, B
dc.creatorVaragić, Vladislav M.
dc.date.accessioned2020-06-03T12:47:06Z
dc.date.available2020-06-03T12:47:06Z
dc.date.issued2003
dc.identifier.issn0379-0355
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/224
dc.description.abstractIn our previous experiments, we demonstrated that xylazine, an alpha(2)-adrenergic agonist, stimulated proliferation of thymocytes triggered by concanavalin A. In contrast, higher concentrations of xylazine were inhibitory. In this work, we studied the mechanisms involved in immunosuppression of xylazine and found that the compound at concentrations between 100 muM and 500 muM induced apoptosis of rat thymocytes in vitro. In addition, xylazine at concentrations higher than 50 muM also induced apoptosis of a thymocyte hybridoma (BWRT8) and increased apoptosis of the line triggered by T cell receptor (TCR) cross-linking. Apoptosis was confirmed by morphological analysis staining with merocyanine 540 and propidium iodide and in cases of BWRT8 by fragmentation of DNA. The mechanisms of xylazine-induced apoptosis of the BWRT8 hybridoma were further examined. We demonstrated that the process in both nonactivated and activated (TCR cross-linking) BWRT8 cells was not prevented by yohimbine (a selective alpha-adrenergic antagonist) and by antibodies to Fas and Fas-L. In contrast, cell death was completely blocked by a caspase inhibitor Z-Val-Ala-Asp (OMe)-CH2F. Cyclosporine, a calcineurin blocker partly inhibited the xylazine-induced apoptosis of activated BWRT8 cells.en
dc.publisherProus Science, Sau-Thomson Reuters, Barcelona
dc.rightsrestrictedAccess
dc.sourceMethods and Findings in Experimental and Clinical Pharmacology
dc.subjectalpha(2)-adrenergic agonisten
dc.subjectapoptosisen
dc.subjectsignalingen
dc.subjectT cell hybridomaen
dc.subjectthymocytesen
dc.titleXylazine, an alpha(2)-adrenergic agonist, induces apoptosis of rat thymocytes and a thymocyte hybridoma line in vitroen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЋупић, Витомир; Јандрић, Д.; Милојковић, Б; Цолић, М; Варагић, Владислав М.;
dc.citation.volume25
dc.citation.issue1
dc.citation.spage5
dc.citation.epage10
dc.citation.other25(1): 5-10
dc.citation.rankM23
dc.identifier.wos000181533700001
dc.identifier.doi10.1358/mf.2003.25.1.772541
dc.identifier.pmid12690700
dc.identifier.scopus2-s2.0-0037223624
dc.identifier.rcubconv_1418
dc.type.versionpublishedVersion


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