Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease
Laboratorijska dijagnostika virusa parainfluence 3 goveda, goveđeg herpesvirusa 1 i goveđeg respiratornog sincicijalnog virusa kao uzročnika respiratornog sindroma goveda
Апстракт
The bovine respiratory disease complex (BRDC) is multifactorial and results from interactions between host factors, environmental factors, and pathogens. A virus, as an initial pathogen alters the animal’s immunity supporting the bacterial colonization of the lower respiratory tract. Bovine herpesvirus 1 (BHV-1), bovine parainfluenza virus 3 (BPIV-3), and bovine respiratory syncytial virus (BRSV) are among the most significant viruses associated with BRDC. The disease most often affects young and older immunosuppressed animals. Laboratory results depend on the selected sampling site of the respiratory tract and proper timing during the period of virus shedding. The samples for testing mostly include nasal or nasopharyngeal swabs, tracheal wash, bronchoalveolar lavage fluid, or necropsy specimens. Virus isolation, although considered as the gold standard, is time-consuming and depends on the virus species and sampling conditions. Most of the virus identification methods used today are m...olecular assays (conventional and real-time PCR or RT-PCR) that are rapid, sensitive, and specific, which is of the essence in veterinary diagnostic laboratories. DNA sequencing is mostly used to detect specific genetic mutations and for molecular epidemiology of disease outbreaks. Serological diagnosis is performed based on the detection of specific antibody presence after infection of seronegative animals or a 4-fold specific antibody titer rise in paired serum samples. Different assays are available, including virus neutralization, complement fixation, haemagglutination inhibition, and ELISA. The early and reliable diagnosis is beneficial in the management and control of BRDC and is the basis of a timely treatment and prevention program.
Кључне речи:
BRDC / BHV-1 / BPIV-3 / BRSV / laboratory diagnosisИзвор:
Biotechnology in Animal Husbandry, 2021, 37, 1, 1-15Издавач:
- Belgrade : Institute for Animal Husbandry
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Nišavić, Jakov AU - Milić, Nenad AU - Radalj, Andrea AU - Stanojković, Aleksandar AU - Veljović, Ljubiša PY - 2021 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2260 AB - The bovine respiratory disease complex (BRDC) is multifactorial and results from interactions between host factors, environmental factors, and pathogens. A virus, as an initial pathogen alters the animal’s immunity supporting the bacterial colonization of the lower respiratory tract. Bovine herpesvirus 1 (BHV-1), bovine parainfluenza virus 3 (BPIV-3), and bovine respiratory syncytial virus (BRSV) are among the most significant viruses associated with BRDC. The disease most often affects young and older immunosuppressed animals. Laboratory results depend on the selected sampling site of the respiratory tract and proper timing during the period of virus shedding. The samples for testing mostly include nasal or nasopharyngeal swabs, tracheal wash, bronchoalveolar lavage fluid, or necropsy specimens. Virus isolation, although considered as the gold standard, is time-consuming and depends on the virus species and sampling conditions. Most of the virus identification methods used today are molecular assays (conventional and real-time PCR or RT-PCR) that are rapid, sensitive, and specific, which is of the essence in veterinary diagnostic laboratories. DNA sequencing is mostly used to detect specific genetic mutations and for molecular epidemiology of disease outbreaks. Serological diagnosis is performed based on the detection of specific antibody presence after infection of seronegative animals or a 4-fold specific antibody titer rise in paired serum samples. Different assays are available, including virus neutralization, complement fixation, haemagglutination inhibition, and ELISA. The early and reliable diagnosis is beneficial in the management and control of BRDC and is the basis of a timely treatment and prevention program. PB - Belgrade : Institute for Animal Husbandry T2 - Biotechnology in Animal Husbandry T1 - Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease T1 - Laboratorijska dijagnostika virusa parainfluence 3 goveda, goveđeg herpesvirusa 1 i goveđeg respiratornog sincicijalnog virusa kao uzročnika respiratornog sindroma goveda VL - 37 IS - 1 SP - 1 EP - 15 DO - 10.2298/BAH2101001N ER -
@article{ author = "Nišavić, Jakov and Milić, Nenad and Radalj, Andrea and Stanojković, Aleksandar and Veljović, Ljubiša", year = "2021", abstract = "The bovine respiratory disease complex (BRDC) is multifactorial and results from interactions between host factors, environmental factors, and pathogens. A virus, as an initial pathogen alters the animal’s immunity supporting the bacterial colonization of the lower respiratory tract. Bovine herpesvirus 1 (BHV-1), bovine parainfluenza virus 3 (BPIV-3), and bovine respiratory syncytial virus (BRSV) are among the most significant viruses associated with BRDC. The disease most often affects young and older immunosuppressed animals. Laboratory results depend on the selected sampling site of the respiratory tract and proper timing during the period of virus shedding. The samples for testing mostly include nasal or nasopharyngeal swabs, tracheal wash, bronchoalveolar lavage fluid, or necropsy specimens. Virus isolation, although considered as the gold standard, is time-consuming and depends on the virus species and sampling conditions. Most of the virus identification methods used today are molecular assays (conventional and real-time PCR or RT-PCR) that are rapid, sensitive, and specific, which is of the essence in veterinary diagnostic laboratories. DNA sequencing is mostly used to detect specific genetic mutations and for molecular epidemiology of disease outbreaks. Serological diagnosis is performed based on the detection of specific antibody presence after infection of seronegative animals or a 4-fold specific antibody titer rise in paired serum samples. Different assays are available, including virus neutralization, complement fixation, haemagglutination inhibition, and ELISA. The early and reliable diagnosis is beneficial in the management and control of BRDC and is the basis of a timely treatment and prevention program.", publisher = "Belgrade : Institute for Animal Husbandry", journal = "Biotechnology in Animal Husbandry", title = "Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease, Laboratorijska dijagnostika virusa parainfluence 3 goveda, goveđeg herpesvirusa 1 i goveđeg respiratornog sincicijalnog virusa kao uzročnika respiratornog sindroma goveda", volume = "37", number = "1", pages = "1-15", doi = "10.2298/BAH2101001N" }
Nišavić, J., Milić, N., Radalj, A., Stanojković, A.,& Veljović, L.. (2021). Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease. in Biotechnology in Animal Husbandry Belgrade : Institute for Animal Husbandry., 37(1), 1-15. https://doi.org/10.2298/BAH2101001N
Nišavić J, Milić N, Radalj A, Stanojković A, Veljović L. Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease. in Biotechnology in Animal Husbandry. 2021;37(1):1-15. doi:10.2298/BAH2101001N .
Nišavić, Jakov, Milić, Nenad, Radalj, Andrea, Stanojković, Aleksandar, Veljović, Ljubiša, "Laboratory diagnostics of bovine parainfluenza-3 virus, bovine herpesvirus 1, and bovine respiratory syncytial virus associated with bovine respiratory disease" in Biotechnology in Animal Husbandry, 37, no. 1 (2021):1-15, https://doi.org/10.2298/BAH2101001N . .