Primena metoda u karakterizaciji nekih sojeva konjskih herpesvirusa tipa 1 i 4 (EHV-1 i EHV-4) sa teritorije Republike Srbije
The Application of Methods in the Characterization of Some Strains of Equine Herpesviruses 1 and 4 (EHV-1 and EHV-4) from the Republic of Serbia
Апстракт
UVOD: Herpesvirusne infekcije su prisutne u populacijama konja širom sveta i najčešće ih
izazivaju konjski herpesvirus 1 (EHV-1) i konjski herpesvirus 4 (EHV-4). Konjski herpesvirus
1 predstavlja jedan od najznačajnijih uzročnika pobačaja gravidnih kobila, povećanog
mortaliteta novorođene ždrebadi, respiratornog oboljenja mladih jedinki i neuroloških
poremećaja. Konjski herpesvirus 4 izaziva respiratornu infekciju konja tzv. rinopneumonitis
koji može naneti velike ekonomske gubitke u konjarstvu zbog smanjene radne sposobnosti
životinja usled dugog perioda oporavka.
CILJ: Identifikacija i molekularna karakterizacija sojeva EHV-1 i EHV-4 u ukupno 40 uzoraka
limfnih čvorova, slezine, kičmene moždine i produžene moždine poreklom od konja sa
teritorije Republike Srbije.
METOD: Identifikacija EHV-1 i EHV-4 primenom metoda lančane reakcije polimeraze (PCR)
sa sekvenciranjem delова genoma navedenih virusa koji kodiraju sintezu glikoproteina B
(gB) primenom metode po Sanger-u i njih...ova filogenetska analiza.
REZULTAT: Nukleinska kiselina EHV-1 je detektovana u ukupno 36 (90%) uzoraka, a EHV-
4 u ukupno 9 (22,5%) ispitivanih uzoraka, dok je prisustvo mešovite infekcije sa EHV-1 i
EHV-4 zabeleženo u ukupno 9 (22,5%) uzoraka. Filogenetskom analizom dobijenih sekvenci
navedenih virusa zabeležena je značajna sličnost sa sojevima EHV-1 i EHV-4 izolovanih kod
konja u Turskoj i Japanu.
ZAKLJUČAK: Dobijeni rezultati potvrđuju opravdanost primene molekularnih metoda
virusološke dijagnostike u cilju brze i precizne identifikacije EHV-1 i EHV-4 i značajno
doprinose boljem poznavanju epizootiološke situacije na teritoriji Republike Srbije što
predstavlja osnovu za preduzimanje preventivnih mera za uspešnu kontrolu oboljenja konja
izazvanih navedenim virusima.
INTRODUCTION: Herpesviral infections are widely distributed in equine populations
worldwide and are most often caused by equine herpesvirus 1 (EHV-1) and equine
herpesvirus 4 (EHV-4). Equine herpesvirus 1 is one of the most significant causative agents
of abortion, neonatal foal mortality, respiratory disease of young horses as well as
neurological disease. Equine herpesvirus 4 causes a respiratory infection ie.
rhinopneumonitis that leads to major economic losses in the equine industry as a result of
prolonged recovery period of diseased horses.
AIM: Identification and molecular characterization of EHV-1 and EHV-4 strains in total of 40
samples of lymph nodes, spleen, spinal cord and medulla collected from horses originating
from The Republic of Serbia.
METHOD: Identification of EHV-1 and EHV-4 by polymerase chain reaction (PCR) with
nucleotide sequencing of the parts of the genome coding the synthesis of glycoprotein B (gB)
and subsequent phylogenetic analysis.
RESULT: T...he nucleic acid of EHV-1 was detected in 36 samples (90%) and EHV-4 in total of
9 examined samples (22.5%). The presence of mixed infection with both viruses was
observed in 9 (22.5%) samples. The phylogenetic analysis of the obtained nucleotide
sequences of EHV-1 and EHV-4 identified in this study showed high similarity with Turkish
and Japanese EHV-1 and EHV-4 strains.
CONCLUSION: The obtained results confirm the applicability of molecular diagnostic
methods in rapid and precise identification of EHV-1 and EHV-4 and provide insight to the
epizootiological situation in The Republic of Serbia as a basis for undertaking of preventive
measures in order to effectively control herpesviral diseases of horses.
Кључне речи:
EHV-1 / EHV-4 / molekularna karakterizacija / molecular characterizationИзвор:
XI Kongres mikrobiologa Srbije MIKROMED 2017, 2017, 38-39Издавач:
- Udruženje mikrobiologa Srbije, Beograd
Колекције
Институција/група
Fakultet veterinarske medicineTY - CONF AU - Radalj, Andrea AU - Milić, Nenad AU - Nišavić, Jakov PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2271 AB - UVOD: Herpesvirusne infekcije su prisutne u populacijama konja širom sveta i najčešće ih izazivaju konjski herpesvirus 1 (EHV-1) i konjski herpesvirus 4 (EHV-4). Konjski herpesvirus 1 predstavlja jedan od najznačajnijih uzročnika pobačaja gravidnih kobila, povećanog mortaliteta novorođene ždrebadi, respiratornog oboljenja mladih jedinki i neuroloških poremećaja. Konjski herpesvirus 4 izaziva respiratornu infekciju konja tzv. rinopneumonitis koji može naneti velike ekonomske gubitke u konjarstvu zbog smanjene radne sposobnosti životinja usled dugog perioda oporavka. CILJ: Identifikacija i molekularna karakterizacija sojeva EHV-1 i EHV-4 u ukupno 40 uzoraka limfnih čvorova, slezine, kičmene moždine i produžene moždine poreklom od konja sa teritorije Republike Srbije. METOD: Identifikacija EHV-1 i EHV-4 primenom metoda lančane reakcije polimeraze (PCR) sa sekvenciranjem delова genoma navedenih virusa koji kodiraju sintezu glikoproteina B (gB) primenom metode po Sanger-u i njihova filogenetska analiza. REZULTAT: Nukleinska kiselina EHV-1 je detektovana u ukupno 36 (90%) uzoraka, a EHV- 4 u ukupno 9 (22,5%) ispitivanih uzoraka, dok je prisustvo mešovite infekcije sa EHV-1 i EHV-4 zabeleženo u ukupno 9 (22,5%) uzoraka. Filogenetskom analizom dobijenih sekvenci navedenih virusa zabeležena je značajna sličnost sa sojevima EHV-1 i EHV-4 izolovanih kod konja u Turskoj i Japanu. ZAKLJUČAK: Dobijeni rezultati potvrđuju opravdanost primene molekularnih metoda virusološke dijagnostike u cilju brze i precizne identifikacije EHV-1 i EHV-4 i značajno doprinose boljem poznavanju epizootiološke situacije na teritoriji Republike Srbije što predstavlja osnovu za preduzimanje preventivnih mera za uspešnu kontrolu oboljenja konja izazvanih navedenim virusima. AB - INTRODUCTION: Herpesviral infections are widely distributed in equine populations worldwide and are most often caused by equine herpesvirus 1 (EHV-1) and equine herpesvirus 4 (EHV-4). Equine herpesvirus 1 is one of the most significant causative agents of abortion, neonatal foal mortality, respiratory disease of young horses as well as neurological disease. Equine herpesvirus 4 causes a respiratory infection ie. rhinopneumonitis that leads to major economic losses in the equine industry as a result of prolonged recovery period of diseased horses. AIM: Identification and molecular characterization of EHV-1 and EHV-4 strains in total of 40 samples of lymph nodes, spleen, spinal cord and medulla collected from horses originating from The Republic of Serbia. METHOD: Identification of EHV-1 and EHV-4 by polymerase chain reaction (PCR) with nucleotide sequencing of the parts of the genome coding the synthesis of glycoprotein B (gB) and subsequent phylogenetic analysis. RESULT: The nucleic acid of EHV-1 was detected in 36 samples (90%) and EHV-4 in total of 9 examined samples (22.5%). The presence of mixed infection with both viruses was observed in 9 (22.5%) samples. The phylogenetic analysis of the obtained nucleotide sequences of EHV-1 and EHV-4 identified in this study showed high similarity with Turkish and Japanese EHV-1 and EHV-4 strains. CONCLUSION: The obtained results confirm the applicability of molecular diagnostic methods in rapid and precise identification of EHV-1 and EHV-4 and provide insight to the epizootiological situation in The Republic of Serbia as a basis for undertaking of preventive measures in order to effectively control herpesviral diseases of horses. PB - Udruženje mikrobiologa Srbije, Beograd C3 - XI Kongres mikrobiologa Srbije MIKROMED 2017 T1 - Primena metoda u karakterizaciji nekih sojeva konjskih herpesvirusa tipa 1 i 4 (EHV-1 i EHV-4) sa teritorije Republike Srbije T1 - The Application of Methods in the Characterization of Some Strains of Equine Herpesviruses 1 and 4 (EHV-1 and EHV-4) from the Republic of Serbia SP - 38 EP - 39 UR - https://hdl.handle.net/21.15107/rcub_veterinar_2271 ER -
@conference{ author = "Radalj, Andrea and Milić, Nenad and Nišavić, Jakov", year = "2017", abstract = "UVOD: Herpesvirusne infekcije su prisutne u populacijama konja širom sveta i najčešće ih izazivaju konjski herpesvirus 1 (EHV-1) i konjski herpesvirus 4 (EHV-4). Konjski herpesvirus 1 predstavlja jedan od najznačajnijih uzročnika pobačaja gravidnih kobila, povećanog mortaliteta novorođene ždrebadi, respiratornog oboljenja mladih jedinki i neuroloških poremećaja. Konjski herpesvirus 4 izaziva respiratornu infekciju konja tzv. rinopneumonitis koji može naneti velike ekonomske gubitke u konjarstvu zbog smanjene radne sposobnosti životinja usled dugog perioda oporavka. CILJ: Identifikacija i molekularna karakterizacija sojeva EHV-1 i EHV-4 u ukupno 40 uzoraka limfnih čvorova, slezine, kičmene moždine i produžene moždine poreklom od konja sa teritorije Republike Srbije. METOD: Identifikacija EHV-1 i EHV-4 primenom metoda lančane reakcije polimeraze (PCR) sa sekvenciranjem delова genoma navedenih virusa koji kodiraju sintezu glikoproteina B (gB) primenom metode po Sanger-u i njihova filogenetska analiza. REZULTAT: Nukleinska kiselina EHV-1 je detektovana u ukupno 36 (90%) uzoraka, a EHV- 4 u ukupno 9 (22,5%) ispitivanih uzoraka, dok je prisustvo mešovite infekcije sa EHV-1 i EHV-4 zabeleženo u ukupno 9 (22,5%) uzoraka. Filogenetskom analizom dobijenih sekvenci navedenih virusa zabeležena je značajna sličnost sa sojevima EHV-1 i EHV-4 izolovanih kod konja u Turskoj i Japanu. ZAKLJUČAK: Dobijeni rezultati potvrđuju opravdanost primene molekularnih metoda virusološke dijagnostike u cilju brze i precizne identifikacije EHV-1 i EHV-4 i značajno doprinose boljem poznavanju epizootiološke situacije na teritoriji Republike Srbije što predstavlja osnovu za preduzimanje preventivnih mera za uspešnu kontrolu oboljenja konja izazvanih navedenim virusima., INTRODUCTION: Herpesviral infections are widely distributed in equine populations worldwide and are most often caused by equine herpesvirus 1 (EHV-1) and equine herpesvirus 4 (EHV-4). Equine herpesvirus 1 is one of the most significant causative agents of abortion, neonatal foal mortality, respiratory disease of young horses as well as neurological disease. Equine herpesvirus 4 causes a respiratory infection ie. rhinopneumonitis that leads to major economic losses in the equine industry as a result of prolonged recovery period of diseased horses. AIM: Identification and molecular characterization of EHV-1 and EHV-4 strains in total of 40 samples of lymph nodes, spleen, spinal cord and medulla collected from horses originating from The Republic of Serbia. METHOD: Identification of EHV-1 and EHV-4 by polymerase chain reaction (PCR) with nucleotide sequencing of the parts of the genome coding the synthesis of glycoprotein B (gB) and subsequent phylogenetic analysis. RESULT: The nucleic acid of EHV-1 was detected in 36 samples (90%) and EHV-4 in total of 9 examined samples (22.5%). The presence of mixed infection with both viruses was observed in 9 (22.5%) samples. The phylogenetic analysis of the obtained nucleotide sequences of EHV-1 and EHV-4 identified in this study showed high similarity with Turkish and Japanese EHV-1 and EHV-4 strains. CONCLUSION: The obtained results confirm the applicability of molecular diagnostic methods in rapid and precise identification of EHV-1 and EHV-4 and provide insight to the epizootiological situation in The Republic of Serbia as a basis for undertaking of preventive measures in order to effectively control herpesviral diseases of horses.", publisher = "Udruženje mikrobiologa Srbije, Beograd", journal = "XI Kongres mikrobiologa Srbije MIKROMED 2017", title = "Primena metoda u karakterizaciji nekih sojeva konjskih herpesvirusa tipa 1 i 4 (EHV-1 i EHV-4) sa teritorije Republike Srbije, The Application of Methods in the Characterization of Some Strains of Equine Herpesviruses 1 and 4 (EHV-1 and EHV-4) from the Republic of Serbia", pages = "38-39", url = "https://hdl.handle.net/21.15107/rcub_veterinar_2271" }
Radalj, A., Milić, N.,& Nišavić, J.. (2017). Primena metoda u karakterizaciji nekih sojeva konjskih herpesvirusa tipa 1 i 4 (EHV-1 i EHV-4) sa teritorije Republike Srbije. in XI Kongres mikrobiologa Srbije MIKROMED 2017 Udruženje mikrobiologa Srbije, Beograd., 38-39. https://hdl.handle.net/21.15107/rcub_veterinar_2271
Radalj A, Milić N, Nišavić J. Primena metoda u karakterizaciji nekih sojeva konjskih herpesvirusa tipa 1 i 4 (EHV-1 i EHV-4) sa teritorije Republike Srbije. in XI Kongres mikrobiologa Srbije MIKROMED 2017. 2017;:38-39. https://hdl.handle.net/21.15107/rcub_veterinar_2271 .
Radalj, Andrea, Milić, Nenad, Nišavić, Jakov, "Primena metoda u karakterizaciji nekih sojeva konjskih herpesvirusa tipa 1 i 4 (EHV-1 i EHV-4) sa teritorije Republike Srbije" in XI Kongres mikrobiologa Srbije MIKROMED 2017 (2017):38-39, https://hdl.handle.net/21.15107/rcub_veterinar_2271 .