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Procena različitih dijagnostičkih metoda za identifikaciju supkliničkog endometritisa mlečnih krava sa patološkim puerperijumom i njihova pouzdanost u predikciji graviditeta

dc.creatorBajagić, Bojana
dc.creatorMrkun, Janko
dc.creatorKirovski, Danijela
dc.creatorSavić, Đorđe
dc.creatorBudimir, Draženko
dc.creatorMaletić, Jelena
dc.creatorMaletić, Milan
dc.date.accessioned2021-12-24T09:35:13Z
dc.date.available2021-12-24T09:35:13Z
dc.date.issued2021
dc.identifier.issn0567-8315
dc.identifier.urihttps://vet-erinar.vet.bg.ac.rs/handle/123456789/2327
dc.description.abstractdiagnoses and their accuracy to predict pregnancy. The study was performed on 51 postpartum cross breed dairy cows with pathological puerperium. Artificial insemination (AI) was done in spontaneous estrus. Endometrium samples were taken by cytotape (CT), cervical mucus (CM) samples by Metricheck and smears for crystallization degree were obtained at AI day. Blood serum samples for progesterone (P4) concentration determination were taken at AI day and 17 days later. Cows were examined by ultrasound 35 days after AI to confirm pregnancy. Percent (%) of polymorphnuclear leukocytes (PMNs) taken by CT were expressed by ROC curve (with cut-off point set at 1%). Out of the total of 51 cows involved in the study, 13.73% true positive and 41.17% true negative cows were found (54.90%). The content of CM sampled with Metricheck was altered in 11 cows (21.57%), and one of them was pregnant (1.96%). An average macroscopic CM value for pregnant cows was 0.06±0.24 vs. 0.39±0.79 for non-pregnant (p<0.05). An average value of CM crystallization in pregnant cows was 2.50±1.04 vs. 1.85±1.15 in non-pregnant (p<0.05). P4 concentration was not statistically different at AI day, but was statistically significantly higher 17 days later in pregnant cows (p = 0.002). CT alterations may be a reliable diagnostic tool for SCE diagnosis, while macroscopic CM alterations may be used for CE detection. P4 determination is beneficial in pregnancy diagnosis while CM crystallization degree is helpful in determination of best time for AI.sr
dc.description.abstractCilj studije bio je procena različitih metoda za dijagnostiku supkliničkog endometritisa (SKE) i njihove pouzdanosti za predviđanje graviditeta. U studiju je bila uključena 51 postpartalna mlečna krava mešane rase sa patološkim puerperijem. Veštačko osemenjavanje (VO) rađeno je u spontanom estrusu. Na dan VO uzeti su uzorci endometrijuma dobijeni cytotape (CT) tehnikom, kao i uzorci cervikalne sluzi (CS) dobijeni upotrebom Metričeka. Razmaz CS, urađen isti dan, korišten je za ispitivanje stepena kristalizacije. Uzorci krvi za određivanje koncentracije progesterona (P4) uzeti su na dan VO i 17 dana kasnije. Krave su pregledane ultrazvukom 35 dana nakon VO radi potvrđivanja graviditeta. Procenat (%) polimorfonuklearnih leukocita (PMNs) dobijenih CT tehnikom je izražen ROC krivom (granična vrednost je određena na 1%). Od ukupno 54,90% krava uključenih u studiju, utvrđeno je 13,73% stvarno pozitivnih i 41,17% stvarno negativnih krava. Izmenjen sadržaj CS uzorkovane Metričekom utvrđen je kod jedanaest krava (21,57%), ali samo jedna (1,96%) je bila gravidna. Prosečna makroskopska vrednost CS kod gravidnih krava je bila 0,06±0,24 u odnosu na 0,39±0,79 kod negravidnih (p<0,05). Prosečna vrednost kristalizacije CS kod gravidnih krava je bila 2,50±1,04 u odnosu na 1,85±1,15 kod negravidnih (p<0,05). Koncentracija P4 nije se statistički razlikovala na dan VO, ali je bila statistički značajno veća 17 dana kasnije kod gravidnih životinja (p = 0.002). Promene utvrđene CT mogu biti indikator SKE, a promene CS dobijene Metričekom mogu biti indikator kliničkog endometritisa. Određivanje P4 korisno je u dijagnostici graviditeta, a kristalizacija CS može se koristiti kao pomoćni metod za određivanje najpovoljnijeg vremena za VO.sr
dc.language.isoensr
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beogradsr
dc.rightsopenAccesssr
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceActa veterinaria-Beogradsr
dc.subjectcervical mucussr
dc.subjectcytotapesr
dc.subjectMetrichecksr
dc.subjectsubclinical endometritissr
dc.subjectpregnancysr
dc.titleAssessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceivesr
dc.titleProcena različitih dijagnostičkih metoda za identifikaciju supkliničkog endometritisa mlečnih krava sa patološkim puerperijumom i njihova pouzdanost u predikciji graviditetasr
dc.typearticlesr
dc.rights.licenseBYsr
dc.citation.volume71
dc.citation.issue4
dc.citation.spage462
dc.citation.epage476
dc.citation.rankM23
dc.identifier.wos000744142000007
dc.identifier.doi10.2478/acve-2021-0038
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/6442/Assessment_of_different_pub_2021.pdf
dc.type.versionpublishedVersionsr


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