New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives
2021
Аутори
Kovačević, ZoranaKladar, Nebojša
Čabarkapa, Ivana
Radinović, Miodrag
Maletić, Milan
Erdeljan, Mihajlo
Božin, Biljana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Mastitis represents a heavy burden for the dairy sector worldwide with high economic and animal welfare impact. Antibiotic treatment is an important component of mastitis control programs. However, emergence and transfer of antimicrobial-resistant (AMR) bacteria is becoming a growing concern. Therefore, the development of novel agents is required for prevention and treatment of mastitis. Hence, our aim was to assess the antibacterial properties of two essential oils (EOs) obtained from oregano (Origanum vulgare L., Lamiaceae) and mountain savory (Satureja montana L., Lamiaceae) against mastitis-associated bacteria in Serbia. The chemical composition and antioxidant potential of these EOs were also evaluated. The present study was conducted on strains derived from aseptic milk samples collected from Holstein-Friesian cows with clinical or subclinical mastitis, during the morning milking. Clinical mastitis was assessed by clinical examination, while subclinical mastitis was confirmed usi...ng somatic cell count in the milk samples. The microdilution method was used to determine the antibacterial activity, while antioxidant potential of the EOs was evaluated in several in vitro assays. The values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were used to quantitatively measure the antibacterial activity of each EO. MIC/MBC ranged from 0.78/6.25 and 0.39/0.78 mg/mL for oregano and mountain savory, respectively. A total of 25 compounds were identified in the oregano EO, while 47 were identified in winter savory EO, among which aromatic oxygenated monoterpenes were the most abundant compounds. The tested EOs have shown promising antimicrobial activity and could be considered as one of the treatment approaches in mastitis-affected cows.
Кључне речи:
Antibacterial activity / Antibiotics / Antioxidant / Essential oil / Oregano / Winter savory / Mastitis-causing bacteriaИзвор:
Antibiotics, 2021, 10, 12Издавач:
- MDPI
Финансирање / пројекти:
- InfoBomat - Intramammary Ethno-Veterinary Formulation in Prevention and Treatment of Bovine Mastitis for Optimization of Antimicrobial Treatment (RS-ScienceFundRS-Promis-6066966)
DOI: 10.3390/antibiotics10121460
ISSN: 2079-6382
WoS: 000741861800001
Scopus: 2-s2.0-85122659945
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Kovačević, Zorana AU - Kladar, Nebojša AU - Čabarkapa, Ivana AU - Radinović, Miodrag AU - Maletić, Milan AU - Erdeljan, Mihajlo AU - Božin, Biljana PY - 2021 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2390 AB - Mastitis represents a heavy burden for the dairy sector worldwide with high economic and animal welfare impact. Antibiotic treatment is an important component of mastitis control programs. However, emergence and transfer of antimicrobial-resistant (AMR) bacteria is becoming a growing concern. Therefore, the development of novel agents is required for prevention and treatment of mastitis. Hence, our aim was to assess the antibacterial properties of two essential oils (EOs) obtained from oregano (Origanum vulgare L., Lamiaceae) and mountain savory (Satureja montana L., Lamiaceae) against mastitis-associated bacteria in Serbia. The chemical composition and antioxidant potential of these EOs were also evaluated. The present study was conducted on strains derived from aseptic milk samples collected from Holstein-Friesian cows with clinical or subclinical mastitis, during the morning milking. Clinical mastitis was assessed by clinical examination, while subclinical mastitis was confirmed using somatic cell count in the milk samples. The microdilution method was used to determine the antibacterial activity, while antioxidant potential of the EOs was evaluated in several in vitro assays. The values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were used to quantitatively measure the antibacterial activity of each EO. MIC/MBC ranged from 0.78/6.25 and 0.39/0.78 mg/mL for oregano and mountain savory, respectively. A total of 25 compounds were identified in the oregano EO, while 47 were identified in winter savory EO, among which aromatic oxygenated monoterpenes were the most abundant compounds. The tested EOs have shown promising antimicrobial activity and could be considered as one of the treatment approaches in mastitis-affected cows. PB - MDPI T2 - Antibiotics T1 - New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives VL - 10 IS - 12 DO - 10.3390/antibiotics10121460 ER -
@article{ author = "Kovačević, Zorana and Kladar, Nebojša and Čabarkapa, Ivana and Radinović, Miodrag and Maletić, Milan and Erdeljan, Mihajlo and Božin, Biljana", year = "2021", abstract = "Mastitis represents a heavy burden for the dairy sector worldwide with high economic and animal welfare impact. Antibiotic treatment is an important component of mastitis control programs. However, emergence and transfer of antimicrobial-resistant (AMR) bacteria is becoming a growing concern. Therefore, the development of novel agents is required for prevention and treatment of mastitis. Hence, our aim was to assess the antibacterial properties of two essential oils (EOs) obtained from oregano (Origanum vulgare L., Lamiaceae) and mountain savory (Satureja montana L., Lamiaceae) against mastitis-associated bacteria in Serbia. The chemical composition and antioxidant potential of these EOs were also evaluated. The present study was conducted on strains derived from aseptic milk samples collected from Holstein-Friesian cows with clinical or subclinical mastitis, during the morning milking. Clinical mastitis was assessed by clinical examination, while subclinical mastitis was confirmed using somatic cell count in the milk samples. The microdilution method was used to determine the antibacterial activity, while antioxidant potential of the EOs was evaluated in several in vitro assays. The values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were used to quantitatively measure the antibacterial activity of each EO. MIC/MBC ranged from 0.78/6.25 and 0.39/0.78 mg/mL for oregano and mountain savory, respectively. A total of 25 compounds were identified in the oregano EO, while 47 were identified in winter savory EO, among which aromatic oxygenated monoterpenes were the most abundant compounds. The tested EOs have shown promising antimicrobial activity and could be considered as one of the treatment approaches in mastitis-affected cows.", publisher = "MDPI", journal = "Antibiotics", title = "New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives", volume = "10", number = "12", doi = "10.3390/antibiotics10121460" }
Kovačević, Z., Kladar, N., Čabarkapa, I., Radinović, M., Maletić, M., Erdeljan, M.,& Božin, B.. (2021). New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives. in Antibiotics MDPI., 10(12). https://doi.org/10.3390/antibiotics10121460
Kovačević Z, Kladar N, Čabarkapa I, Radinović M, Maletić M, Erdeljan M, Božin B. New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives. in Antibiotics. 2021;10(12). doi:10.3390/antibiotics10121460 .
Kovačević, Zorana, Kladar, Nebojša, Čabarkapa, Ivana, Radinović, Miodrag, Maletić, Milan, Erdeljan, Mihajlo, Božin, Biljana, "New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives" in Antibiotics, 10, no. 12 (2021), https://doi.org/10.3390/antibiotics10121460 . .