The detection and phylogenetic analysis of equine herpesviruses 1, 4 and 5 identified in nasal swab samples of asymptomatic horses from Serbia and Bosnia and Herzegovina
Апстракт
Nasal swabs originating from 112 apparently clinically healthy and unvaccinated horses
of different age, breed and from diverse rearing conditions from Serbia and Bosnia and
Herzegovina were examined for the presence of equine herpesviruses 1, 4 and 5 using
multiplex nested PCR (Mn‑PCR) and virus isolation. The detected viruses were subsequently
characterised by gB gene nucleotide sequencing and their phylogenetic analysis was
performed. The infections with EHV‑1, EHV‑4, and EHV‑5 in the examined horse populations
are apparently chronic, subclinical and persistent, whilst the shedding of EHV‑1 and EHV‑5
was confirmed by their successful isolation. A connection was established between the
finding of EHVs and rearing conditions since horses kept together in stables were positive
for at least one EHV in contrast to animals held free grazing or individually. EHV‑5 was found
most often in younger horses, however descending in frequency in animals up to 10 years
of age. The phylog...enetic analysis showed that the identified EHV strains group mostly with
Turkish and German strains of respective viruses. A certain degree of genetic heterogeneity
was determined regarding the identified EHV‑5 strains in contrast to EHV‑1 and EHV‑4.
Кључне речи:
Asymptomatic horses / EHV-1 / EHV-4 / EHV-5 / Phylogenetic analysisИзвор:
Veterinaria Italiana, 2022, 57, 4, 265-274Издавач:
- Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Radalj, Andrea AU - Milić, Nenad AU - Stevanović, Oliver AU - Nišavić, Jakov PY - 2022 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2455 AB - Nasal swabs originating from 112 apparently clinically healthy and unvaccinated horses of different age, breed and from diverse rearing conditions from Serbia and Bosnia and Herzegovina were examined for the presence of equine herpesviruses 1, 4 and 5 using multiplex nested PCR (Mn‑PCR) and virus isolation. The detected viruses were subsequently characterised by gB gene nucleotide sequencing and their phylogenetic analysis was performed. The infections with EHV‑1, EHV‑4, and EHV‑5 in the examined horse populations are apparently chronic, subclinical and persistent, whilst the shedding of EHV‑1 and EHV‑5 was confirmed by their successful isolation. A connection was established between the finding of EHVs and rearing conditions since horses kept together in stables were positive for at least one EHV in contrast to animals held free grazing or individually. EHV‑5 was found most often in younger horses, however descending in frequency in animals up to 10 years of age. The phylogenetic analysis showed that the identified EHV strains group mostly with Turkish and German strains of respective viruses. A certain degree of genetic heterogeneity was determined regarding the identified EHV‑5 strains in contrast to EHV‑1 and EHV‑4. PB - Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise T2 - Veterinaria Italiana T1 - The detection and phylogenetic analysis of equine herpesviruses 1, 4 and 5 identified in nasal swab samples of asymptomatic horses from Serbia and Bosnia and Herzegovina VL - 57 IS - 4 SP - 265 EP - 274 DO - 10.12834/VetIt.1767.9329.3 ER -
@article{ author = "Radalj, Andrea and Milić, Nenad and Stevanović, Oliver and Nišavić, Jakov", year = "2022", abstract = "Nasal swabs originating from 112 apparently clinically healthy and unvaccinated horses of different age, breed and from diverse rearing conditions from Serbia and Bosnia and Herzegovina were examined for the presence of equine herpesviruses 1, 4 and 5 using multiplex nested PCR (Mn‑PCR) and virus isolation. The detected viruses were subsequently characterised by gB gene nucleotide sequencing and their phylogenetic analysis was performed. The infections with EHV‑1, EHV‑4, and EHV‑5 in the examined horse populations are apparently chronic, subclinical and persistent, whilst the shedding of EHV‑1 and EHV‑5 was confirmed by their successful isolation. A connection was established between the finding of EHVs and rearing conditions since horses kept together in stables were positive for at least one EHV in contrast to animals held free grazing or individually. EHV‑5 was found most often in younger horses, however descending in frequency in animals up to 10 years of age. The phylogenetic analysis showed that the identified EHV strains group mostly with Turkish and German strains of respective viruses. A certain degree of genetic heterogeneity was determined regarding the identified EHV‑5 strains in contrast to EHV‑1 and EHV‑4.", publisher = "Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise", journal = "Veterinaria Italiana", title = "The detection and phylogenetic analysis of equine herpesviruses 1, 4 and 5 identified in nasal swab samples of asymptomatic horses from Serbia and Bosnia and Herzegovina", volume = "57", number = "4", pages = "265-274", doi = "10.12834/VetIt.1767.9329.3" }
Radalj, A., Milić, N., Stevanović, O.,& Nišavić, J.. (2022). The detection and phylogenetic analysis of equine herpesviruses 1, 4 and 5 identified in nasal swab samples of asymptomatic horses from Serbia and Bosnia and Herzegovina. in Veterinaria Italiana Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise., 57(4), 265-274. https://doi.org/10.12834/VetIt.1767.9329.3
Radalj A, Milić N, Stevanović O, Nišavić J. The detection and phylogenetic analysis of equine herpesviruses 1, 4 and 5 identified in nasal swab samples of asymptomatic horses from Serbia and Bosnia and Herzegovina. in Veterinaria Italiana. 2022;57(4):265-274. doi:10.12834/VetIt.1767.9329.3 .
Radalj, Andrea, Milić, Nenad, Stevanović, Oliver, Nišavić, Jakov, "The detection and phylogenetic analysis of equine herpesviruses 1, 4 and 5 identified in nasal swab samples of asymptomatic horses from Serbia and Bosnia and Herzegovina" in Veterinaria Italiana, 57, no. 4 (2022):265-274, https://doi.org/10.12834/VetIt.1767.9329.3 . .