Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees
2022
Аутори
Glavinić, UrošBlagojević, Jovan
Ristanić, Marko
Stevanović, Jevrosima
Lakić, Nada
Mirilović, Milorad
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Nosema ceranae is the most widespread microsporidian species which infects the honey bees of Apis mellifera by causing the weakening of their colonies and a decline in their productive and reproductive capacities. The only registered product for its control is the antibiotic fumagillin; however, in the European Union, there is no formulation registered for use in beekeeping. Thymol (3-hydroxy-p-cymene) is a natural essential-oil ingredient derived from Thymus vulgaris, which has been used in Varroa control for decades. The aim of this study was to investigate the effect of thymol supplementation on the expression of immune-related genes and the parameters of oxidative stress and bee survival, as well as spore loads in bees infected with the microsporidian parasite N. ceranae. The results reveal mostly positive effects of thymol on health (increasing levels of immune-related genes and values of oxidative stress parameters, and decreasing Nosema spore loads) when applied to Nosema-infect...ed bees. Moreover, supplementation with thymol did not induce negative effects in Nosema-infected bees. However, our results indicate that in Nosema-free bees, thymol itself could cause certain disorders (affecting bee survival, decreasing oxidative capacity, and downregulation of some immune-related gene expressions), showing that one should be careful with preventive, uncontrolled, and excessive use of thymol. Thus, further research is needed to reveal the effect of this phytogenic supplement on the immunity of uninfected bees.
Кључне речи:
Nosema ceranae / honey bee / immune-related gene expression / oxidative stress / thymolИзвор:
Insects, 2022, 13, 7, 574-Издавач:
- MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
Напомена:
- Supplementary information: https://vet-erinar.vet.bg.ac.rs/handle/123456789/2468
Повезане информације:
- Повезани садржај
https://vet-erinar.vet.bg.ac.rs/handle/123456789/2468
DOI: 10.3390/insects13070574
ISSN: 2075-4450
WoS: 000832203200001
Scopus: 2-s2.0-85133229118
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Glavinić, Uroš AU - Blagojević, Jovan AU - Ristanić, Marko AU - Stevanović, Jevrosima AU - Lakić, Nada AU - Mirilović, Milorad AU - Stanimirović, Zoran PY - 2022 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2467 AB - Nosema ceranae is the most widespread microsporidian species which infects the honey bees of Apis mellifera by causing the weakening of their colonies and a decline in their productive and reproductive capacities. The only registered product for its control is the antibiotic fumagillin; however, in the European Union, there is no formulation registered for use in beekeeping. Thymol (3-hydroxy-p-cymene) is a natural essential-oil ingredient derived from Thymus vulgaris, which has been used in Varroa control for decades. The aim of this study was to investigate the effect of thymol supplementation on the expression of immune-related genes and the parameters of oxidative stress and bee survival, as well as spore loads in bees infected with the microsporidian parasite N. ceranae. The results reveal mostly positive effects of thymol on health (increasing levels of immune-related genes and values of oxidative stress parameters, and decreasing Nosema spore loads) when applied to Nosema-infected bees. Moreover, supplementation with thymol did not induce negative effects in Nosema-infected bees. However, our results indicate that in Nosema-free bees, thymol itself could cause certain disorders (affecting bee survival, decreasing oxidative capacity, and downregulation of some immune-related gene expressions), showing that one should be careful with preventive, uncontrolled, and excessive use of thymol. Thus, further research is needed to reveal the effect of this phytogenic supplement on the immunity of uninfected bees. PB - MDPI T2 - Insects T1 - Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees VL - 13 IS - 7 SP - 574 DO - 10.3390/insects13070574 ER -
@article{ author = "Glavinić, Uroš and Blagojević, Jovan and Ristanić, Marko and Stevanović, Jevrosima and Lakić, Nada and Mirilović, Milorad and Stanimirović, Zoran", year = "2022", abstract = "Nosema ceranae is the most widespread microsporidian species which infects the honey bees of Apis mellifera by causing the weakening of their colonies and a decline in their productive and reproductive capacities. The only registered product for its control is the antibiotic fumagillin; however, in the European Union, there is no formulation registered for use in beekeeping. Thymol (3-hydroxy-p-cymene) is a natural essential-oil ingredient derived from Thymus vulgaris, which has been used in Varroa control for decades. The aim of this study was to investigate the effect of thymol supplementation on the expression of immune-related genes and the parameters of oxidative stress and bee survival, as well as spore loads in bees infected with the microsporidian parasite N. ceranae. The results reveal mostly positive effects of thymol on health (increasing levels of immune-related genes and values of oxidative stress parameters, and decreasing Nosema spore loads) when applied to Nosema-infected bees. Moreover, supplementation with thymol did not induce negative effects in Nosema-infected bees. However, our results indicate that in Nosema-free bees, thymol itself could cause certain disorders (affecting bee survival, decreasing oxidative capacity, and downregulation of some immune-related gene expressions), showing that one should be careful with preventive, uncontrolled, and excessive use of thymol. Thus, further research is needed to reveal the effect of this phytogenic supplement on the immunity of uninfected bees.", publisher = "MDPI", journal = "Insects", title = "Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees", volume = "13", number = "7", pages = "574", doi = "10.3390/insects13070574" }
Glavinić, U., Blagojević, J., Ristanić, M., Stevanović, J., Lakić, N., Mirilović, M.,& Stanimirović, Z.. (2022). Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees. in Insects MDPI., 13(7), 574. https://doi.org/10.3390/insects13070574
Glavinić U, Blagojević J, Ristanić M, Stevanović J, Lakić N, Mirilović M, Stanimirović Z. Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees. in Insects. 2022;13(7):574. doi:10.3390/insects13070574 .
Glavinić, Uroš, Blagojević, Jovan, Ristanić, Marko, Stevanović, Jevrosima, Lakić, Nada, Mirilović, Milorad, Stanimirović, Zoran, "Use of Thymol in Nosema ceranae Control and Health Improvement of Infected Honey Bees" in Insects, 13, no. 7 (2022):574, https://doi.org/10.3390/insects13070574 . .