Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat
2022
Authors
Vasilev, S.Suvajdžić, Branko
Mitic, I.
Sabljić, Lj.
Karabasil, Nedjeljko
Sofronić Milosavljević, Lj.
Vasilev, Dragan
Article (Published version)
Metadata
Show full item recordAbstract
Trichinellosis is an important worldwide foodborne zoonosis. The gold standard test to detect Trichinella spp. larvae in muscle samples of animals intended for human consumption is the artificial digestion method. Handling and dispensing of conventional pepsin powder present significant safety risks for analysts. The use of pepsin powder that is resistant to aerosolization should alleviate these safety concerns. The aim of this study was to compare the efficacy of an aerosol-resistant pepsin powder to conventional pepsin powder in the artificial digestion method. Proficiency samples of pork diaphragm containing specific numbers of viable Trichinella spiralis larvae were tested in two laboratories. The results revealed that aerosol-resistant pepsin was simple, effective and convenient to use, and showed good solubility and larval recovery that met the requirements of the European Union regulation EU 2015/1375. Overall, the efficacy of the aerosol-resistant pepsin was comparable to the c...onventional pepsin and safer for analysts.
Keywords:
digestion method / pepsin / TrichinellaSource:
Journal of Helminthology, 2022, 96, e71-Publisher:
- Cambridge University Press
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200019 (University of Belgrade, Institute for the Application of Nuclear Energy - INEP) (RS-MESTD-inst-2020-200019)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200143 (University of Belgrade, Faculty of Veterinary Medicine) (RS-MESTD-inst-2020-200143)
DOI: 10.1017/S0022149X2200061X
ISSN: 0022-149X; 1475-2697
PubMed: 36189624
Scopus: 2-s2.0-85139099103
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Fakultet veterinarske medicineTY - JOUR AU - Vasilev, S. AU - Suvajdžić, Branko AU - Mitic, I. AU - Sabljić, Lj. AU - Karabasil, Nedjeljko AU - Sofronić Milosavljević, Lj. AU - Vasilev, Dragan PY - 2022 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2495 AB - Trichinellosis is an important worldwide foodborne zoonosis. The gold standard test to detect Trichinella spp. larvae in muscle samples of animals intended for human consumption is the artificial digestion method. Handling and dispensing of conventional pepsin powder present significant safety risks for analysts. The use of pepsin powder that is resistant to aerosolization should alleviate these safety concerns. The aim of this study was to compare the efficacy of an aerosol-resistant pepsin powder to conventional pepsin powder in the artificial digestion method. Proficiency samples of pork diaphragm containing specific numbers of viable Trichinella spiralis larvae were tested in two laboratories. The results revealed that aerosol-resistant pepsin was simple, effective and convenient to use, and showed good solubility and larval recovery that met the requirements of the European Union regulation EU 2015/1375. Overall, the efficacy of the aerosol-resistant pepsin was comparable to the conventional pepsin and safer for analysts. PB - Cambridge University Press T2 - Journal of Helminthology T1 - Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat VL - 96 SP - e71 DO - 10.1017/S0022149X2200061X ER -
@article{ author = "Vasilev, S. and Suvajdžić, Branko and Mitic, I. and Sabljić, Lj. and Karabasil, Nedjeljko and Sofronić Milosavljević, Lj. and Vasilev, Dragan", year = "2022", abstract = "Trichinellosis is an important worldwide foodborne zoonosis. The gold standard test to detect Trichinella spp. larvae in muscle samples of animals intended for human consumption is the artificial digestion method. Handling and dispensing of conventional pepsin powder present significant safety risks for analysts. The use of pepsin powder that is resistant to aerosolization should alleviate these safety concerns. The aim of this study was to compare the efficacy of an aerosol-resistant pepsin powder to conventional pepsin powder in the artificial digestion method. Proficiency samples of pork diaphragm containing specific numbers of viable Trichinella spiralis larvae were tested in two laboratories. The results revealed that aerosol-resistant pepsin was simple, effective and convenient to use, and showed good solubility and larval recovery that met the requirements of the European Union regulation EU 2015/1375. Overall, the efficacy of the aerosol-resistant pepsin was comparable to the conventional pepsin and safer for analysts.", publisher = "Cambridge University Press", journal = "Journal of Helminthology", title = "Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat", volume = "96", pages = "e71", doi = "10.1017/S0022149X2200061X" }
Vasilev, S., Suvajdžić, B., Mitic, I., Sabljić, Lj., Karabasil, N., Sofronić Milosavljević, Lj.,& Vasilev, D.. (2022). Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat. in Journal of Helminthology Cambridge University Press., 96, e71. https://doi.org/10.1017/S0022149X2200061X
Vasilev S, Suvajdžić B, Mitic I, Sabljić L, Karabasil N, Sofronić Milosavljević L, Vasilev D. Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat. in Journal of Helminthology. 2022;96:e71. doi:10.1017/S0022149X2200061X .
Vasilev, S., Suvajdžić, Branko, Mitic, I., Sabljić, Lj., Karabasil, Nedjeljko, Sofronić Milosavljević, Lj., Vasilev, Dragan, "Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat" in Journal of Helminthology, 96 (2022):e71, https://doi.org/10.1017/S0022149X2200061X . .