Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia
Само за регистроване кориснике
2022
Аутори
Sukara, RatkoAndrić, Nenad
Francuski Andrić, Jelena
Mihaljica, Darko
Veinović, Gorana
Ranković, Vladan
Tomanović, Snežana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background
The epidemiological status concerning many canine tick-borne diseases (TBDs) in Serbia is still insufficiently known.
Objectives
Our study aimed to investigate the presence of tick-borne pathogens of the family Anaplasmataceae and Hepatozoon spp., as a cause of illnesses accompanied by clinical signs that can occur in dogs with anaplasmosis, ehrlichiosis and hepatozoonosis.
Methods
Dogs are included in the study based on the presence of a minimum of three clinical and/or pathological findings that could be associated with anaplasmosis, ehrlichiosis and hepatozoonosis. During the study (April–October 2018), 11 dogs met the conditions to be included in the survey. Identification of the causative agent in the blood of diseased dogs was performed by conventional PCR followed by sequencing.
Results
The presence of the pathogens was confirmed in three animals (3/11, 27.3%). The presence of Ehrlichia canis was confirmed in 3-month-old female Rottweiler puppy, an 8-year... old Miniature Schnauzer female was positive for Hepatozoon canis infection, while 4-year-old mixed breed male dog was co-infected with both mentioned pathogens. These are the first cases of autochthonous infection with E. canis and H. canis in dogs from Serbia confirmed by molecular methods.
Conclusions
The results of our study indicate the importance of molecular methods to establish a reliable diagnosis of TBDs. Also, the confirmed presence of causative agents of canine monocytic ehrlichiosis and hepatozoonosis in Serbia appeals to veterinary practitioners that it is necessary to exclude the presence of those diseases in suspicious dogs.
Кључне речи:
dogs / Ehrlichia canis / Hepatozoon canis / polymerase chain reaction / Serbia / tick-borne diseasesИзвор:
Veterinary Medicine and Science, 2022, 9, 1, 111-118Издавач:
- John Wiley & Sons Ltd.
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200015 (Универзитет у Београду, Институт за медицинска истраживања) (RS-MESTD-inst-2020-200015)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Sukara, Ratko AU - Andrić, Nenad AU - Francuski Andrić, Jelena AU - Mihaljica, Darko AU - Veinović, Gorana AU - Ranković, Vladan AU - Tomanović, Snežana PY - 2022 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2511 AB - Background The epidemiological status concerning many canine tick-borne diseases (TBDs) in Serbia is still insufficiently known. Objectives Our study aimed to investigate the presence of tick-borne pathogens of the family Anaplasmataceae and Hepatozoon spp., as a cause of illnesses accompanied by clinical signs that can occur in dogs with anaplasmosis, ehrlichiosis and hepatozoonosis. Methods Dogs are included in the study based on the presence of a minimum of three clinical and/or pathological findings that could be associated with anaplasmosis, ehrlichiosis and hepatozoonosis. During the study (April–October 2018), 11 dogs met the conditions to be included in the survey. Identification of the causative agent in the blood of diseased dogs was performed by conventional PCR followed by sequencing. Results The presence of the pathogens was confirmed in three animals (3/11, 27.3%). The presence of Ehrlichia canis was confirmed in 3-month-old female Rottweiler puppy, an 8-year old Miniature Schnauzer female was positive for Hepatozoon canis infection, while 4-year-old mixed breed male dog was co-infected with both mentioned pathogens. These are the first cases of autochthonous infection with E. canis and H. canis in dogs from Serbia confirmed by molecular methods. Conclusions The results of our study indicate the importance of molecular methods to establish a reliable diagnosis of TBDs. Also, the confirmed presence of causative agents of canine monocytic ehrlichiosis and hepatozoonosis in Serbia appeals to veterinary practitioners that it is necessary to exclude the presence of those diseases in suspicious dogs. PB - John Wiley & Sons Ltd. T2 - Veterinary Medicine and Science T1 - Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia VL - 9 IS - 1 IS - 111 SP - 111 EP - 118 DO - 10.1002/vms3.1061 ER -
@article{ author = "Sukara, Ratko and Andrić, Nenad and Francuski Andrić, Jelena and Mihaljica, Darko and Veinović, Gorana and Ranković, Vladan and Tomanović, Snežana", year = "2022", abstract = "Background The epidemiological status concerning many canine tick-borne diseases (TBDs) in Serbia is still insufficiently known. Objectives Our study aimed to investigate the presence of tick-borne pathogens of the family Anaplasmataceae and Hepatozoon spp., as a cause of illnesses accompanied by clinical signs that can occur in dogs with anaplasmosis, ehrlichiosis and hepatozoonosis. Methods Dogs are included in the study based on the presence of a minimum of three clinical and/or pathological findings that could be associated with anaplasmosis, ehrlichiosis and hepatozoonosis. During the study (April–October 2018), 11 dogs met the conditions to be included in the survey. Identification of the causative agent in the blood of diseased dogs was performed by conventional PCR followed by sequencing. Results The presence of the pathogens was confirmed in three animals (3/11, 27.3%). The presence of Ehrlichia canis was confirmed in 3-month-old female Rottweiler puppy, an 8-year old Miniature Schnauzer female was positive for Hepatozoon canis infection, while 4-year-old mixed breed male dog was co-infected with both mentioned pathogens. These are the first cases of autochthonous infection with E. canis and H. canis in dogs from Serbia confirmed by molecular methods. Conclusions The results of our study indicate the importance of molecular methods to establish a reliable diagnosis of TBDs. Also, the confirmed presence of causative agents of canine monocytic ehrlichiosis and hepatozoonosis in Serbia appeals to veterinary practitioners that it is necessary to exclude the presence of those diseases in suspicious dogs.", publisher = "John Wiley & Sons Ltd.", journal = "Veterinary Medicine and Science", title = "Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia", volume = "9", number = "1, 111", pages = "111-118", doi = "10.1002/vms3.1061" }
Sukara, R., Andrić, N., Francuski Andrić, J., Mihaljica, D., Veinović, G., Ranković, V.,& Tomanović, S.. (2022). Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia. in Veterinary Medicine and Science John Wiley & Sons Ltd.., 9(1), 111-118. https://doi.org/10.1002/vms3.1061
Sukara R, Andrić N, Francuski Andrić J, Mihaljica D, Veinović G, Ranković V, Tomanović S. Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia. in Veterinary Medicine and Science. 2022;9(1):111-118. doi:10.1002/vms3.1061 .
Sukara, Ratko, Andrić, Nenad, Francuski Andrić, Jelena, Mihaljica, Darko, Veinović, Gorana, Ranković, Vladan, Tomanović, Snežana, "Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia" in Veterinary Medicine and Science, 9, no. 1 (2022):111-118, https://doi.org/10.1002/vms3.1061 . .