Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats
Аутори
Lužajić Božinovski, TijanaTodorović, Vera
Milošević, Ivan
Prokić, Bogomir Bolka
Gajdov, Vladimir
Nešović, Katarina
Mišković-Stanković, Vesna
Marković, Danica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Biocompatibility of materials is one of the most important conditions for their successful application in tissue regeneration
and repair. Cell-surface interactions stimulate adhesion and activation of macrophages whose acquaintance can assist in
designing novel biomaterials that promote favorable macrophage–biomaterial surface interactions for clinical application.
This study is designed to determine the distribution and number of macrophages as a means of biocompatibility evaluation
of two newly synthesized materials [silver/poly(vinyl alcohol) (Ag/PVA) and silver/poly(vinyl alcohol)/graphene (Ag/PVA/
Gr) nanocomposite hydrogels] in vivo, with approval of the Ethics Committee of the Faculty of Veterinary Medicine,
University of Belgrade. Macrophages and giant cells were analyzed in tissue sections stained by routine H&E and im munohistochemical methods (CD68+
). Statistical relevance was determined in the statistical software package SPSS 20
(IBM corp). The results of the stud...y in terms of the number of giant cells localized around the implant showed that their
number was highest on the seventh postoperative day (p.o.d.) in the group implanted with Ag/PVA hydrogels, and on the
30th p.o.d. in the group implanted with Ag/PVA/Gr. Interestingly, the number of macrophages measured in the capsular and
pericapsular space was highest in the group implanted with the commercial Suprasorb© material. The increased mac rophage number, registered around the Ag/PVA/Gr implant on 60th p.o.d. indicates that the addition of graphene can, in a
specific way, modulate different biological responses of tissues in the process of wound healing, regeneration, and
integration.
Кључне речи:
Histology / in vivo / macrophages / rats / silver/poly(vinyl alcohol)graphene / skinИзвор:
Journal of Biomaterials Applications, 2022, 36, 6, 1111-1125Финансирање / пројекти:
- Антиоксидативна заштита и потенцијали за диференцијацију и регенерацију мезенхималних матичних ћелија из различитих ткива током процеса старења (RS-MESTD-Basic Research (BR or ON)-175061)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Lužajić Božinovski, Tijana AU - Todorović, Vera AU - Milošević, Ivan AU - Prokić, Bogomir Bolka AU - Gajdov, Vladimir AU - Nešović, Katarina AU - Mišković-Stanković, Vesna AU - Marković, Danica PY - 2022 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2531 AB - Biocompatibility of materials is one of the most important conditions for their successful application in tissue regeneration and repair. Cell-surface interactions stimulate adhesion and activation of macrophages whose acquaintance can assist in designing novel biomaterials that promote favorable macrophage–biomaterial surface interactions for clinical application. This study is designed to determine the distribution and number of macrophages as a means of biocompatibility evaluation of two newly synthesized materials [silver/poly(vinyl alcohol) (Ag/PVA) and silver/poly(vinyl alcohol)/graphene (Ag/PVA/ Gr) nanocomposite hydrogels] in vivo, with approval of the Ethics Committee of the Faculty of Veterinary Medicine, University of Belgrade. Macrophages and giant cells were analyzed in tissue sections stained by routine H&E and im munohistochemical methods (CD68+ ). Statistical relevance was determined in the statistical software package SPSS 20 (IBM corp). The results of the study in terms of the number of giant cells localized around the implant showed that their number was highest on the seventh postoperative day (p.o.d.) in the group implanted with Ag/PVA hydrogels, and on the 30th p.o.d. in the group implanted with Ag/PVA/Gr. Interestingly, the number of macrophages measured in the capsular and pericapsular space was highest in the group implanted with the commercial Suprasorb© material. The increased mac rophage number, registered around the Ag/PVA/Gr implant on 60th p.o.d. indicates that the addition of graphene can, in a specific way, modulate different biological responses of tissues in the process of wound healing, regeneration, and integration. T2 - Journal of Biomaterials Applications T1 - Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats VL - 36 IS - 6 SP - 1111 EP - 1125 DO - 10.1177%2F08853282211046119 ER -
@article{ author = "Lužajić Božinovski, Tijana and Todorović, Vera and Milošević, Ivan and Prokić, Bogomir Bolka and Gajdov, Vladimir and Nešović, Katarina and Mišković-Stanković, Vesna and Marković, Danica", year = "2022", abstract = "Biocompatibility of materials is one of the most important conditions for their successful application in tissue regeneration and repair. Cell-surface interactions stimulate adhesion and activation of macrophages whose acquaintance can assist in designing novel biomaterials that promote favorable macrophage–biomaterial surface interactions for clinical application. This study is designed to determine the distribution and number of macrophages as a means of biocompatibility evaluation of two newly synthesized materials [silver/poly(vinyl alcohol) (Ag/PVA) and silver/poly(vinyl alcohol)/graphene (Ag/PVA/ Gr) nanocomposite hydrogels] in vivo, with approval of the Ethics Committee of the Faculty of Veterinary Medicine, University of Belgrade. Macrophages and giant cells were analyzed in tissue sections stained by routine H&E and im munohistochemical methods (CD68+ ). Statistical relevance was determined in the statistical software package SPSS 20 (IBM corp). The results of the study in terms of the number of giant cells localized around the implant showed that their number was highest on the seventh postoperative day (p.o.d.) in the group implanted with Ag/PVA hydrogels, and on the 30th p.o.d. in the group implanted with Ag/PVA/Gr. Interestingly, the number of macrophages measured in the capsular and pericapsular space was highest in the group implanted with the commercial Suprasorb© material. The increased mac rophage number, registered around the Ag/PVA/Gr implant on 60th p.o.d. indicates that the addition of graphene can, in a specific way, modulate different biological responses of tissues in the process of wound healing, regeneration, and integration.", journal = "Journal of Biomaterials Applications", title = "Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats", volume = "36", number = "6", pages = "1111-1125", doi = "10.1177%2F08853282211046119" }
Lužajić Božinovski, T., Todorović, V., Milošević, I., Prokić, B. B., Gajdov, V., Nešović, K., Mišković-Stanković, V.,& Marković, D.. (2022). Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats. in Journal of Biomaterials Applications, 36(6), 1111-1125. https://doi.org/10.1177%2F08853282211046119
Lužajić Božinovski T, Todorović V, Milošević I, Prokić BB, Gajdov V, Nešović K, Mišković-Stanković V, Marković D. Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats. in Journal of Biomaterials Applications. 2022;36(6):1111-1125. doi:10.1177%2F08853282211046119 .
Lužajić Božinovski, Tijana, Todorović, Vera, Milošević, Ivan, Prokić, Bogomir Bolka, Gajdov, Vladimir, Nešović, Katarina, Mišković-Stanković, Vesna, Marković, Danica, "Macrophages, the main marker in biocompatibility evaluation of new hydrogels after subcutaneous implantation in rats" in Journal of Biomaterials Applications, 36, no. 6 (2022):1111-1125, https://doi.org/10.1177%2F08853282211046119 . .