First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus)
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2022
Authors
Kureljušić, BranislavMilićević, Vesna
Ćirović, Duško
Kuručki, Milica
Glišić, Dimitrije
Sapundžić Zurovac, Zorana
Milovanović, Bojan
Weissenbacher-Lang, Christiane
Article (Published version)
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Forty-six golden jackals (Canis aureus) were collected between November 2020 and February 2021 in five counties of Serbia. Lung samples were screened for the presence of Pneumocystis DNA by pan-Pneumocystis PCR on the mtLSU rRNA gene obtaining PCR products of 370 bp in length. Pneumocystis DNA was detected in the lungs from 6/46 (13.04%) golden jackals. Four were females and two were males; four were classified as adults and two as subadults. Positive samples were confirmed in 4/5 investigated counties. No gross pathologic lung lesions were observed in this study. The sequences of Pneumocystis spp. from golden jackals were identical to one another and showed the highest similarity with Pneumocystis spp. sequences of dogs (98% nucleotide identity). The genetic variation was comparable to Pneumocystis spp. of raccoon dogs (95-97% nucleotide identity), red foxes (91-95% nucleotide identity), ferrets (86% nucleotide identity), and another Pneumocystis type in dogs (P. canis Ck2, 81% nucleo...tide identity) was higher. Golden jackals may be carriers and may play a nonnegligible role in the spread of Pneumocystis spp. Although this finding cannot be directly related to any clinical manifestation or pathologic lesions, a possible role in the exacerbation of different pulmonary disorders should be considered.
Keywords:
Golden jackal / mtLSU rRNA gene / phylogenetic analysis / Pneumocystis spp. / SerbiaSource:
Journal of wildlife diseases, 2022, 58, 4, 897-901Publisher:
- Wildlife Disease Association
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200030 (Scientific Veterinary Institute of Serbia, Belgrade) (RS-MESTD-inst-2020-200030)
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Fakultet veterinarske medicineTY - JOUR AU - Kureljušić, Branislav AU - Milićević, Vesna AU - Ćirović, Duško AU - Kuručki, Milica AU - Glišić, Dimitrije AU - Sapundžić Zurovac, Zorana AU - Milovanović, Bojan AU - Weissenbacher-Lang, Christiane PY - 2022 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2545 AB - Forty-six golden jackals (Canis aureus) were collected between November 2020 and February 2021 in five counties of Serbia. Lung samples were screened for the presence of Pneumocystis DNA by pan-Pneumocystis PCR on the mtLSU rRNA gene obtaining PCR products of 370 bp in length. Pneumocystis DNA was detected in the lungs from 6/46 (13.04%) golden jackals. Four were females and two were males; four were classified as adults and two as subadults. Positive samples were confirmed in 4/5 investigated counties. No gross pathologic lung lesions were observed in this study. The sequences of Pneumocystis spp. from golden jackals were identical to one another and showed the highest similarity with Pneumocystis spp. sequences of dogs (98% nucleotide identity). The genetic variation was comparable to Pneumocystis spp. of raccoon dogs (95-97% nucleotide identity), red foxes (91-95% nucleotide identity), ferrets (86% nucleotide identity), and another Pneumocystis type in dogs (P. canis Ck2, 81% nucleotide identity) was higher. Golden jackals may be carriers and may play a nonnegligible role in the spread of Pneumocystis spp. Although this finding cannot be directly related to any clinical manifestation or pathologic lesions, a possible role in the exacerbation of different pulmonary disorders should be considered. PB - Wildlife Disease Association T2 - Journal of wildlife diseases T1 - First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus) VL - 58 IS - 4 SP - 897 EP - 901 DO - 10.7589/JWD-D-22-00007 ER -
@article{ author = "Kureljušić, Branislav and Milićević, Vesna and Ćirović, Duško and Kuručki, Milica and Glišić, Dimitrije and Sapundžić Zurovac, Zorana and Milovanović, Bojan and Weissenbacher-Lang, Christiane", year = "2022", abstract = "Forty-six golden jackals (Canis aureus) were collected between November 2020 and February 2021 in five counties of Serbia. Lung samples were screened for the presence of Pneumocystis DNA by pan-Pneumocystis PCR on the mtLSU rRNA gene obtaining PCR products of 370 bp in length. Pneumocystis DNA was detected in the lungs from 6/46 (13.04%) golden jackals. Four were females and two were males; four were classified as adults and two as subadults. Positive samples were confirmed in 4/5 investigated counties. No gross pathologic lung lesions were observed in this study. The sequences of Pneumocystis spp. from golden jackals were identical to one another and showed the highest similarity with Pneumocystis spp. sequences of dogs (98% nucleotide identity). The genetic variation was comparable to Pneumocystis spp. of raccoon dogs (95-97% nucleotide identity), red foxes (91-95% nucleotide identity), ferrets (86% nucleotide identity), and another Pneumocystis type in dogs (P. canis Ck2, 81% nucleotide identity) was higher. Golden jackals may be carriers and may play a nonnegligible role in the spread of Pneumocystis spp. Although this finding cannot be directly related to any clinical manifestation or pathologic lesions, a possible role in the exacerbation of different pulmonary disorders should be considered.", publisher = "Wildlife Disease Association", journal = "Journal of wildlife diseases", title = "First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus)", volume = "58", number = "4", pages = "897-901", doi = "10.7589/JWD-D-22-00007" }
Kureljušić, B., Milićević, V., Ćirović, D., Kuručki, M., Glišić, D., Sapundžić Zurovac, Z., Milovanović, B.,& Weissenbacher-Lang, C.. (2022). First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus). in Journal of wildlife diseases Wildlife Disease Association., 58(4), 897-901. https://doi.org/10.7589/JWD-D-22-00007
Kureljušić B, Milićević V, Ćirović D, Kuručki M, Glišić D, Sapundžić Zurovac Z, Milovanović B, Weissenbacher-Lang C. First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus). in Journal of wildlife diseases. 2022;58(4):897-901. doi:10.7589/JWD-D-22-00007 .
Kureljušić, Branislav, Milićević, Vesna, Ćirović, Duško, Kuručki, Milica, Glišić, Dimitrije, Sapundžić Zurovac, Zorana, Milovanović, Bojan, Weissenbacher-Lang, Christiane, "First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus)" in Journal of wildlife diseases, 58, no. 4 (2022):897-901, https://doi.org/10.7589/JWD-D-22-00007 . .