Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro
Само за регистроване кориснике
2019
Аутори
Đelić, NinoslavRadaković, Milena
Borozan, Sunčica
Dimirijević-Srećković, Vesna
Pajović, Nevena
Vejnović, Branislav
Borozan, Nevena
Bankoglu, Ezgi Eylül
Stopper, Helga
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Diabetes represents one of the major health concerns, especially in developed countries. Some hormones such as
the stress hormone adrenaline can induce reactive oxygen species (ROS) and may worsen the diabetes.
Therefore, the main aim of the investigation was to find out whether peripheral blood mononuclear cells
(PBMCs) from normal persons have less DNA damage induced by adrenaline (0.1, 1 and 10 μM) in comparison to
PBMCs from obese, prediabetic and diabetic patients. Also, the biochemical parameters of oxidative stress
(TBARS, catalase) and lactate dehydrogenase were monitored. It was observed that higher concentrations of
adrenaline (1 and 10 μM) induced DNA damage in the obese, prediabetic and diabetic groups. In healthy individuals only the highest concentration of adrenaline caused significant increase in the DNA damage. In
summary, total comet score (TCS) comparison has shown significant differences between groups, and DNA
damaging effects of adrenaline were most eviden...t in diabetic patients. The results of the biochemical analysis
also demonstrate that adrenaline exerts most obvious effects in diabetic individuals which is manifested as
significant change of parameters of oxidative stress. In summary, the obtained results demonstrated that diabetics are more sensitive to genotoxic effects of adrenaline and this effect probably resulted from decreased
antioxidative defence mechanisms in various stages of progression through diabetes. Therefore, these results
could contribute to a better understanding of a role of endocrine factors to damage of cellular biomolecules
which could be useful in finding novel therapeutic approaches and lifestyle changes with an aim to lower the
possibility of diabetes complications.
Кључне речи:
Diabetes / Oxidative stress / DNA damage / PBMC / AdrenalineИзвор:
Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 2019, 843, 81-89Издавач:
- Elsevier
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- COST action hCOMET [CA 15132]
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Đelić, Ninoslav AU - Radaković, Milena AU - Borozan, Sunčica AU - Dimirijević-Srećković, Vesna AU - Pajović, Nevena AU - Vejnović, Branislav AU - Borozan, Nevena AU - Bankoglu, Ezgi Eylül AU - Stopper, Helga AU - Stanimirović, Zoran PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2546 AB - Diabetes represents one of the major health concerns, especially in developed countries. Some hormones such as the stress hormone adrenaline can induce reactive oxygen species (ROS) and may worsen the diabetes. Therefore, the main aim of the investigation was to find out whether peripheral blood mononuclear cells (PBMCs) from normal persons have less DNA damage induced by adrenaline (0.1, 1 and 10 μM) in comparison to PBMCs from obese, prediabetic and diabetic patients. Also, the biochemical parameters of oxidative stress (TBARS, catalase) and lactate dehydrogenase were monitored. It was observed that higher concentrations of adrenaline (1 and 10 μM) induced DNA damage in the obese, prediabetic and diabetic groups. In healthy individuals only the highest concentration of adrenaline caused significant increase in the DNA damage. In summary, total comet score (TCS) comparison has shown significant differences between groups, and DNA damaging effects of adrenaline were most evident in diabetic patients. The results of the biochemical analysis also demonstrate that adrenaline exerts most obvious effects in diabetic individuals which is manifested as significant change of parameters of oxidative stress. In summary, the obtained results demonstrated that diabetics are more sensitive to genotoxic effects of adrenaline and this effect probably resulted from decreased antioxidative defence mechanisms in various stages of progression through diabetes. Therefore, these results could contribute to a better understanding of a role of endocrine factors to damage of cellular biomolecules which could be useful in finding novel therapeutic approaches and lifestyle changes with an aim to lower the possibility of diabetes complications. PB - Elsevier T2 - Mutation Research: Genetic Toxicology and Environmental Mutagenesis T1 - Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro VL - 843 SP - 81 EP - 89 DO - 10.1016/j.mrgentox.2019.01.013 ER -
@article{ author = "Đelić, Ninoslav and Radaković, Milena and Borozan, Sunčica and Dimirijević-Srećković, Vesna and Pajović, Nevena and Vejnović, Branislav and Borozan, Nevena and Bankoglu, Ezgi Eylül and Stopper, Helga and Stanimirović, Zoran", year = "2019", abstract = "Diabetes represents one of the major health concerns, especially in developed countries. Some hormones such as the stress hormone adrenaline can induce reactive oxygen species (ROS) and may worsen the diabetes. Therefore, the main aim of the investigation was to find out whether peripheral blood mononuclear cells (PBMCs) from normal persons have less DNA damage induced by adrenaline (0.1, 1 and 10 μM) in comparison to PBMCs from obese, prediabetic and diabetic patients. Also, the biochemical parameters of oxidative stress (TBARS, catalase) and lactate dehydrogenase were monitored. It was observed that higher concentrations of adrenaline (1 and 10 μM) induced DNA damage in the obese, prediabetic and diabetic groups. In healthy individuals only the highest concentration of adrenaline caused significant increase in the DNA damage. In summary, total comet score (TCS) comparison has shown significant differences between groups, and DNA damaging effects of adrenaline were most evident in diabetic patients. The results of the biochemical analysis also demonstrate that adrenaline exerts most obvious effects in diabetic individuals which is manifested as significant change of parameters of oxidative stress. In summary, the obtained results demonstrated that diabetics are more sensitive to genotoxic effects of adrenaline and this effect probably resulted from decreased antioxidative defence mechanisms in various stages of progression through diabetes. Therefore, these results could contribute to a better understanding of a role of endocrine factors to damage of cellular biomolecules which could be useful in finding novel therapeutic approaches and lifestyle changes with an aim to lower the possibility of diabetes complications.", publisher = "Elsevier", journal = "Mutation Research: Genetic Toxicology and Environmental Mutagenesis", title = "Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro", volume = "843", pages = "81-89", doi = "10.1016/j.mrgentox.2019.01.013" }
Đelić, N., Radaković, M., Borozan, S., Dimirijević-Srećković, V., Pajović, N., Vejnović, B., Borozan, N., Bankoglu, E. E., Stopper, H.,& Stanimirović, Z.. (2019). Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro. in Mutation Research: Genetic Toxicology and Environmental Mutagenesis Elsevier., 843, 81-89. https://doi.org/10.1016/j.mrgentox.2019.01.013
Đelić N, Radaković M, Borozan S, Dimirijević-Srećković V, Pajović N, Vejnović B, Borozan N, Bankoglu EE, Stopper H, Stanimirović Z. Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro. in Mutation Research: Genetic Toxicology and Environmental Mutagenesis. 2019;843:81-89. doi:10.1016/j.mrgentox.2019.01.013 .
Đelić, Ninoslav, Radaković, Milena, Borozan, Sunčica, Dimirijević-Srećković, Vesna, Pajović, Nevena, Vejnović, Branislav, Borozan, Nevena, Bankoglu, Ezgi Eylül, Stopper, Helga, Stanimirović, Zoran, "Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro" in Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 843 (2019):81-89, https://doi.org/10.1016/j.mrgentox.2019.01.013 . .