Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (hn) and fusion (f) glycoprotein antigens of parainfluenza 3 virus, in vitro
Ispitivanje antigenske strukture i nekih bioloških aktivnosti HN i F antigena virusa Parainfluence 3, in vitro
Апстракт
The objective of our study was examination of the antigenic structure fusional and hemolytic activities of the surface glycoprotein HN and F antigens of purified parainfluenza (PI 3) viruses activated with 0.025 g/dl trypsin-versen (molecular weights of 112 kD, 81-82 kD and 30-31 kD), in vitro. The samples of activated PI3 virions with total protein concentrations of 0.55 and 0.27mg/ml and hemagglutinating titre of 256 and 128 HAU/0.1 ml, induced bovine turbinate(BT) cell fusion and hemolysis of guinea-pig erythrocytes. After treatment of the aforementioned samples with specific hyperimmune sera against PI 3 virus in which the hemagglutination-inhibition (HI) titre was 1:64, cell fusion was not registered. The results show that there are possibilities to use fusional and hemolytic tests for the fast detection of imunologically important glycoprotein antigens of PI 3 viruses and their identification with specific hyperimmune sera.
Cilj naših istraživanja je bilo ispitivanje antigenske strukture, fuzionih i hemolitičkih aktivnosti površinskih glikoproteinskih HN i F antigena prečišćenih virusa PI3 aktivisanih sa 0.025g/dl tripsin-versenom (molekulske težine od 112kDa, 81-82 kDa i 30-31 kDa), in vitro. Uzorci aktivisanih virusa PI 3 sa ukupnim koncentracijama proteina od 0.55 i 0.27 mg/ml i hemaglutinacionog titra od 256 i 128 HJ/0.1ml, indukovali su fuziju BT ćelija i hemolizu eritrocita zamorca. Posle tretiranja napred navedenih uzoraka specifičnim hiperimunim serumom protiv virusa PI 3 čiji je HI titar bio 1:64, nije registrovana ćelijska fuzija. Rezultati pokazuju da postoji mogućnost korišćenja fuzionog i hemolitičkog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa PI 3 i njihovu identifikaciju sa specifičnim hiperimunim serumom.
Кључне речи:
PI3 virus / glycoprotein antigens / SDS-PAGE / BT cells / fusional test / hemolysisИзвор:
Acta Veterinaria-Beograd, 2003, 53, 5-6, 321-332Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
DOI: 10.2298/AVB0306321M
ISSN: 0567-8315
WoS: 000187620300004
Scopus: 2-s2.0-1642534322
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Milić, Nenad AU - Gađanski-Omerović, Gordana AU - Ašanin, Ružica AU - Nišavić, Jakov AU - Radojičić, Marina PY - 2003 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/256 AB - The objective of our study was examination of the antigenic structure fusional and hemolytic activities of the surface glycoprotein HN and F antigens of purified parainfluenza (PI 3) viruses activated with 0.025 g/dl trypsin-versen (molecular weights of 112 kD, 81-82 kD and 30-31 kD), in vitro. The samples of activated PI3 virions with total protein concentrations of 0.55 and 0.27mg/ml and hemagglutinating titre of 256 and 128 HAU/0.1 ml, induced bovine turbinate(BT) cell fusion and hemolysis of guinea-pig erythrocytes. After treatment of the aforementioned samples with specific hyperimmune sera against PI 3 virus in which the hemagglutination-inhibition (HI) titre was 1:64, cell fusion was not registered. The results show that there are possibilities to use fusional and hemolytic tests for the fast detection of imunologically important glycoprotein antigens of PI 3 viruses and their identification with specific hyperimmune sera. AB - Cilj naših istraživanja je bilo ispitivanje antigenske strukture, fuzionih i hemolitičkih aktivnosti površinskih glikoproteinskih HN i F antigena prečišćenih virusa PI3 aktivisanih sa 0.025g/dl tripsin-versenom (molekulske težine od 112kDa, 81-82 kDa i 30-31 kDa), in vitro. Uzorci aktivisanih virusa PI 3 sa ukupnim koncentracijama proteina od 0.55 i 0.27 mg/ml i hemaglutinacionog titra od 256 i 128 HJ/0.1ml, indukovali su fuziju BT ćelija i hemolizu eritrocita zamorca. Posle tretiranja napred navedenih uzoraka specifičnim hiperimunim serumom protiv virusa PI 3 čiji je HI titar bio 1:64, nije registrovana ćelijska fuzija. Rezultati pokazuju da postoji mogućnost korišćenja fuzionog i hemolitičkog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa PI 3 i njihovu identifikaciju sa specifičnim hiperimunim serumom. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (hn) and fusion (f) glycoprotein antigens of parainfluenza 3 virus, in vitro T1 - Ispitivanje antigenske strukture i nekih bioloških aktivnosti HN i F antigena virusa Parainfluence 3, in vitro VL - 53 IS - 5-6 SP - 321 EP - 332 DO - 10.2298/AVB0306321M ER -
@article{ author = "Milić, Nenad and Gađanski-Omerović, Gordana and Ašanin, Ružica and Nišavić, Jakov and Radojičić, Marina", year = "2003", abstract = "The objective of our study was examination of the antigenic structure fusional and hemolytic activities of the surface glycoprotein HN and F antigens of purified parainfluenza (PI 3) viruses activated with 0.025 g/dl trypsin-versen (molecular weights of 112 kD, 81-82 kD and 30-31 kD), in vitro. The samples of activated PI3 virions with total protein concentrations of 0.55 and 0.27mg/ml and hemagglutinating titre of 256 and 128 HAU/0.1 ml, induced bovine turbinate(BT) cell fusion and hemolysis of guinea-pig erythrocytes. After treatment of the aforementioned samples with specific hyperimmune sera against PI 3 virus in which the hemagglutination-inhibition (HI) titre was 1:64, cell fusion was not registered. The results show that there are possibilities to use fusional and hemolytic tests for the fast detection of imunologically important glycoprotein antigens of PI 3 viruses and their identification with specific hyperimmune sera., Cilj naših istraživanja je bilo ispitivanje antigenske strukture, fuzionih i hemolitičkih aktivnosti površinskih glikoproteinskih HN i F antigena prečišćenih virusa PI3 aktivisanih sa 0.025g/dl tripsin-versenom (molekulske težine od 112kDa, 81-82 kDa i 30-31 kDa), in vitro. Uzorci aktivisanih virusa PI 3 sa ukupnim koncentracijama proteina od 0.55 i 0.27 mg/ml i hemaglutinacionog titra od 256 i 128 HJ/0.1ml, indukovali su fuziju BT ćelija i hemolizu eritrocita zamorca. Posle tretiranja napred navedenih uzoraka specifičnim hiperimunim serumom protiv virusa PI 3 čiji je HI titar bio 1:64, nije registrovana ćelijska fuzija. Rezultati pokazuju da postoji mogućnost korišćenja fuzionog i hemolitičkog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa PI 3 i njihovu identifikaciju sa specifičnim hiperimunim serumom.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (hn) and fusion (f) glycoprotein antigens of parainfluenza 3 virus, in vitro, Ispitivanje antigenske strukture i nekih bioloških aktivnosti HN i F antigena virusa Parainfluence 3, in vitro", volume = "53", number = "5-6", pages = "321-332", doi = "10.2298/AVB0306321M" }
Milić, N., Gađanski-Omerović, G., Ašanin, R., Nišavić, J.,& Radojičić, M.. (2003). Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (hn) and fusion (f) glycoprotein antigens of parainfluenza 3 virus, in vitro. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(5-6), 321-332. https://doi.org/10.2298/AVB0306321M
Milić N, Gađanski-Omerović G, Ašanin R, Nišavić J, Radojičić M. Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (hn) and fusion (f) glycoprotein antigens of parainfluenza 3 virus, in vitro. in Acta Veterinaria-Beograd. 2003;53(5-6):321-332. doi:10.2298/AVB0306321M .
Milić, Nenad, Gađanski-Omerović, Gordana, Ašanin, Ružica, Nišavić, Jakov, Radojičić, Marina, "Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (hn) and fusion (f) glycoprotein antigens of parainfluenza 3 virus, in vitro" in Acta Veterinaria-Beograd, 53, no. 5-6 (2003):321-332, https://doi.org/10.2298/AVB0306321M . .