Examination of antigenic structure and some biological activities of hemagglutinin-neuraminidase (hn) and fusion (f) glycoprotein antigens of parainfluenza 3 virus, in vitro

Abstract

The objective of our study was examination of the antigenic structure fusional and hemolytic activities of the surface glycoprotein HN and F antigens of purified parainfluenza (PI 3) viruses activated with 0.025 g/dl trypsin-versen (molecular weights of 112 kD, 81-82 kD and 30-31 kD), in vitro. The samples of activated PI3 virions with total protein concentrations of 0.55 and 0.27mg/ml and hemagglutinating titre of 256 and 128 HAU/0.1 ml, induced bovine turbinate(BT) cell fusion and hemolysis of guinea-pig erythrocytes. After treatment of the aforementioned samples with specific hyperimmune sera against PI 3 virus in which the hemagglutination-inhibition (HI) titre was 1:64, cell fusion was not registered. The results show that there are possibilities to use fusional and hemolytic tests for the fast detection of imunologically important glycoprotein antigens of PI 3 viruses and their identification with specific hyperimmune sera.

Cilj naših istraživanja je bilo ispitivanje antigenske strukture, fuzionih i hemolitičkih aktivnosti površinskih glikoproteinskih HN i F antigena prečišćenih virusa PI3 aktivisanih sa 0.025g/dl tripsin-versenom (molekulske težine od 112kDa, 81-82 kDa i 30-31 kDa), in vitro. Uzorci aktivisanih virusa PI 3 sa ukupnim koncentracijama proteina od 0.55 i 0.27 mg/ml i hemaglutinacionog titra od 256 i 128 HJ/0.1ml, indukovali su fuziju BT ćelija i hemolizu eritrocita zamorca. Posle tretiranja napred navedenih uzoraka specifičnim hiperimunim serumom protiv virusa PI 3 čiji je HI titar bio 1:64, nije registrovana ćelijska fuzija. Rezultati pokazuju da postoji mogućnost korišćenja fuzionog i hemolitičkog testa za brzu detekciju imunološki značajnih glikoproteinskih antigena virusa PI 3 i njihovu identifikaciju sa specifičnim hiperimunim serumom.