Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection
Аутори
Danilović Luković, JelenaRadovanović, Anita
Milošević, Ivan
Lužajić Božinovski, Tijana
Milanović, Svetlana
Kovačević Filipović, Milica
Korać, Aleksandra
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The stimulative effects of thyroid hormones on mitochondria are realized through
both non-genomic and genomic mechanisms, affecting respiration, mitochondrial plasticity
and biogenesis. The subclinical form of maternal hypothyroidism in rats induces significant
reduction of mitochondria number but also an augmentation of their area in neonatal and
early infantile offspring dyctiotene oocytes. This study aimed to investigate if this form of
subclinical hypothyroidism affects mitochondrial morphology and distribution in the early
prophase of meiosis I oocytes. It was performed on newborn control (C) (n=10) and
hypothyroid (SCH) (n=10) female rat pups derived from control (n=6) and propylthiouracil
treated pregnant dams (n=6), respectively. Ovaries of all pups were removed and processed
for transmission electron microscopy. The morphological features of mitochondria in the
early prophase I oocytes until dyctiotene were assessed. No substantial differences were
found i...n leptotene and zygotene oocytes in SCH group comparing to control, except just a
few mitochondria characterized with shortened cristae, presence of wide pale area centrally
positioned and membrane disruption. Pachytene mitochondria in treated pup oocytes were
in great extent with disrupted membrane, shortened cristae and wide pale area centrally
positioned while these features were rarely observed in control ones. Our results confirm
altered mitochondria morphology found in primordial and primary follicles in case of
maternal hypothyroidism, indicating their impaired function and possibly, propensity to
programmed cell death. Further investigations may indicate to what extent pachytene, as a
meiotic checkpoint, appears to be a milestone possibly predetermining the future of the
cell.
Извор:
Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade, 2018, 103-103Издавач:
- Belgrade : Serbian Society for Mitochondrial and Free Radical Physiology
- Belgrade : Ministry of Education, Science and Technological Development
- Belgrade : University of Belgrade
- Belgrade : Faculty of Biology of University of Belgrade
Финансирање / пројекти:
- Антиоксидативна заштита и потенцијали за диференцијацију и регенерацију мезенхималних матичних ћелија из различитих ткива током процеса старења (RS-MESTD-Basic Research (BR or ON)-175061)
Напомена:
- Book of Abstracts
Колекције
Институција/група
Fakultet veterinarske medicineTY - CONF AU - Danilović Luković, Jelena AU - Radovanović, Anita AU - Milošević, Ivan AU - Lužajić Božinovski, Tijana AU - Milanović, Svetlana AU - Kovačević Filipović, Milica AU - Korać, Aleksandra PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2597 AB - The stimulative effects of thyroid hormones on mitochondria are realized through both non-genomic and genomic mechanisms, affecting respiration, mitochondrial plasticity and biogenesis. The subclinical form of maternal hypothyroidism in rats induces significant reduction of mitochondria number but also an augmentation of their area in neonatal and early infantile offspring dyctiotene oocytes. This study aimed to investigate if this form of subclinical hypothyroidism affects mitochondrial morphology and distribution in the early prophase of meiosis I oocytes. It was performed on newborn control (C) (n=10) and hypothyroid (SCH) (n=10) female rat pups derived from control (n=6) and propylthiouracil treated pregnant dams (n=6), respectively. Ovaries of all pups were removed and processed for transmission electron microscopy. The morphological features of mitochondria in the early prophase I oocytes until dyctiotene were assessed. No substantial differences were found in leptotene and zygotene oocytes in SCH group comparing to control, except just a few mitochondria characterized with shortened cristae, presence of wide pale area centrally positioned and membrane disruption. Pachytene mitochondria in treated pup oocytes were in great extent with disrupted membrane, shortened cristae and wide pale area centrally positioned while these features were rarely observed in control ones. Our results confirm altered mitochondria morphology found in primordial and primary follicles in case of maternal hypothyroidism, indicating their impaired function and possibly, propensity to programmed cell death. Further investigations may indicate to what extent pachytene, as a meiotic checkpoint, appears to be a milestone possibly predetermining the future of the cell. PB - Belgrade : Serbian Society for Mitochondrial and Free Radical Physiology PB - Belgrade : Ministry of Education, Science and Technological Development PB - Belgrade : University of Belgrade PB - Belgrade : Faculty of Biology of University of Belgrade C3 - Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade T1 - Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection SP - 103 EP - 103 UR - https://hdl.handle.net/21.15107/rcub_veterinar_2597 ER -
@conference{ author = "Danilović Luković, Jelena and Radovanović, Anita and Milošević, Ivan and Lužajić Božinovski, Tijana and Milanović, Svetlana and Kovačević Filipović, Milica and Korać, Aleksandra", year = "2018", abstract = "The stimulative effects of thyroid hormones on mitochondria are realized through both non-genomic and genomic mechanisms, affecting respiration, mitochondrial plasticity and biogenesis. The subclinical form of maternal hypothyroidism in rats induces significant reduction of mitochondria number but also an augmentation of their area in neonatal and early infantile offspring dyctiotene oocytes. This study aimed to investigate if this form of subclinical hypothyroidism affects mitochondrial morphology and distribution in the early prophase of meiosis I oocytes. It was performed on newborn control (C) (n=10) and hypothyroid (SCH) (n=10) female rat pups derived from control (n=6) and propylthiouracil treated pregnant dams (n=6), respectively. Ovaries of all pups were removed and processed for transmission electron microscopy. The morphological features of mitochondria in the early prophase I oocytes until dyctiotene were assessed. No substantial differences were found in leptotene and zygotene oocytes in SCH group comparing to control, except just a few mitochondria characterized with shortened cristae, presence of wide pale area centrally positioned and membrane disruption. Pachytene mitochondria in treated pup oocytes were in great extent with disrupted membrane, shortened cristae and wide pale area centrally positioned while these features were rarely observed in control ones. Our results confirm altered mitochondria morphology found in primordial and primary follicles in case of maternal hypothyroidism, indicating their impaired function and possibly, propensity to programmed cell death. Further investigations may indicate to what extent pachytene, as a meiotic checkpoint, appears to be a milestone possibly predetermining the future of the cell.", publisher = "Belgrade : Serbian Society for Mitochondrial and Free Radical Physiology, Belgrade : Ministry of Education, Science and Technological Development, Belgrade : University of Belgrade, Belgrade : Faculty of Biology of University of Belgrade", journal = "Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade", title = "Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection", pages = "103-103", url = "https://hdl.handle.net/21.15107/rcub_veterinar_2597" }
Danilović Luković, J., Radovanović, A., Milošević, I., Lužajić Božinovski, T., Milanović, S., Kovačević Filipović, M.,& Korać, A.. (2018). Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection. in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade Belgrade : Serbian Society for Mitochondrial and Free Radical Physiology., 103-103. https://hdl.handle.net/21.15107/rcub_veterinar_2597
Danilović Luković J, Radovanović A, Milošević I, Lužajić Božinovski T, Milanović S, Kovačević Filipović M, Korać A. Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection. in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade. 2018;:103-103. https://hdl.handle.net/21.15107/rcub_veterinar_2597 .
Danilović Luković, Jelena, Radovanović, Anita, Milošević, Ivan, Lužajić Božinovski, Tijana, Milanović, Svetlana, Kovačević Filipović, Milica, Korać, Aleksandra, "Mitochondria in pachytene: the fragile point of maternal subclinical hypothyroidism affection" in Fourth Congress "Challenges in redox biology", September 28-30. 2018, Belgrade (2018):103-103, https://hdl.handle.net/21.15107/rcub_veterinar_2597 .