Homocysteine concentration in the serum of dogs naturally infected with Leishmania spp. – association with the stage of the disease, therapy, and clinical pathology data
Authors
Antić, MilutinBeletić, Anđelo
Radaković, Milena
Spariosu, Kristina
Stanković, Sanja
Trailović, Dragiša
Kovačević Filipović, Milica
Article (Published version)
Metadata
Show full item recordAbstract
Progressive tissue injury in canine leishmaniosis (CL) is related to the deposition of
immune complexes, which induces vasculitis and leads to endothelial dysfunction.
Homocysteine (Hcy) increase may worsen endothelial dysfunction, but data concerning
its concentration in different CL stages and links to the acute phase response and
oxidative stress are missing. We compared Hcy levels between dogs with mild (N=24)
and moderate CL without treatment (N=17) and treated with anti-Leishmania drugs
and vitamin B supplements (N=9). Dogs with moderate CL, regardless of therapy
administration, had more distinct clinical signs, lower erythron values, and a higher level
of acute-phase proteins (APPs), IgG against Leishmania spp., urea and creatinine, than
dogs with mild CL. Hcy values did not differ between stages, but treated dogs had the
lowest levels of Hcy. An inverse relationship existed between Hcy and the CL stage,
therapy, levels of IgG, and clinical pathology data. The only po...sitive relationship existed
between Hcy and the erythron state. The disease stage and therapeutic intervention
were not related to the oxidative stress level, except in the case of paraoxonase-1/
Hcy ratio, indicating favorable conditions for antioxidative defense in treated dogs. In
conclusion, changes in Hcy levels indicated its possible involvement with endothelial
dysfunction and inverse relationship to tissue injury evaluated by APPs. Finally, Hcy
might be an early marker of favorable conditions for endothelium recovery in CL
Keywords:
allopurinol / B vitamins / homocysteine / Leishmania spp / miltefosinSource:
Acta Veterinaria, 2022, 72, 3, 309-323Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200143 (University of Belgrade, Faculty of Veterinary Medicine) (RS-MESTD-inst-2020-200143)
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Antić, Milutin AU - Beletić, Anđelo AU - Radaković, Milena AU - Spariosu, Kristina AU - Stanković, Sanja AU - Trailović, Dragiša AU - Kovačević Filipović, Milica PY - 2022 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2718 AB - Progressive tissue injury in canine leishmaniosis (CL) is related to the deposition of immune complexes, which induces vasculitis and leads to endothelial dysfunction. Homocysteine (Hcy) increase may worsen endothelial dysfunction, but data concerning its concentration in different CL stages and links to the acute phase response and oxidative stress are missing. We compared Hcy levels between dogs with mild (N=24) and moderate CL without treatment (N=17) and treated with anti-Leishmania drugs and vitamin B supplements (N=9). Dogs with moderate CL, regardless of therapy administration, had more distinct clinical signs, lower erythron values, and a higher level of acute-phase proteins (APPs), IgG against Leishmania spp., urea and creatinine, than dogs with mild CL. Hcy values did not differ between stages, but treated dogs had the lowest levels of Hcy. An inverse relationship existed between Hcy and the CL stage, therapy, levels of IgG, and clinical pathology data. The only positive relationship existed between Hcy and the erythron state. The disease stage and therapeutic intervention were not related to the oxidative stress level, except in the case of paraoxonase-1/ Hcy ratio, indicating favorable conditions for antioxidative defense in treated dogs. In conclusion, changes in Hcy levels indicated its possible involvement with endothelial dysfunction and inverse relationship to tissue injury evaluated by APPs. Finally, Hcy might be an early marker of favorable conditions for endothelium recovery in CL PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria T1 - Homocysteine concentration in the serum of dogs naturally infected with Leishmania spp. – association with the stage of the disease, therapy, and clinical pathology data VL - 72 IS - 3 SP - 309 EP - 323 DO - 10.2478/acve-2022-0025 ER -
@article{ author = "Antić, Milutin and Beletić, Anđelo and Radaković, Milena and Spariosu, Kristina and Stanković, Sanja and Trailović, Dragiša and Kovačević Filipović, Milica", year = "2022", abstract = "Progressive tissue injury in canine leishmaniosis (CL) is related to the deposition of immune complexes, which induces vasculitis and leads to endothelial dysfunction. Homocysteine (Hcy) increase may worsen endothelial dysfunction, but data concerning its concentration in different CL stages and links to the acute phase response and oxidative stress are missing. We compared Hcy levels between dogs with mild (N=24) and moderate CL without treatment (N=17) and treated with anti-Leishmania drugs and vitamin B supplements (N=9). Dogs with moderate CL, regardless of therapy administration, had more distinct clinical signs, lower erythron values, and a higher level of acute-phase proteins (APPs), IgG against Leishmania spp., urea and creatinine, than dogs with mild CL. Hcy values did not differ between stages, but treated dogs had the lowest levels of Hcy. An inverse relationship existed between Hcy and the CL stage, therapy, levels of IgG, and clinical pathology data. The only positive relationship existed between Hcy and the erythron state. The disease stage and therapeutic intervention were not related to the oxidative stress level, except in the case of paraoxonase-1/ Hcy ratio, indicating favorable conditions for antioxidative defense in treated dogs. In conclusion, changes in Hcy levels indicated its possible involvement with endothelial dysfunction and inverse relationship to tissue injury evaluated by APPs. Finally, Hcy might be an early marker of favorable conditions for endothelium recovery in CL", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria", title = "Homocysteine concentration in the serum of dogs naturally infected with Leishmania spp. – association with the stage of the disease, therapy, and clinical pathology data", volume = "72", number = "3", pages = "309-323", doi = "10.2478/acve-2022-0025" }
Antić, M., Beletić, A., Radaković, M., Spariosu, K., Stanković, S., Trailović, D.,& Kovačević Filipović, M.. (2022). Homocysteine concentration in the serum of dogs naturally infected with Leishmania spp. – association with the stage of the disease, therapy, and clinical pathology data. in Acta Veterinaria Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 72(3), 309-323. https://doi.org/10.2478/acve-2022-0025
Antić M, Beletić A, Radaković M, Spariosu K, Stanković S, Trailović D, Kovačević Filipović M. Homocysteine concentration in the serum of dogs naturally infected with Leishmania spp. – association with the stage of the disease, therapy, and clinical pathology data. in Acta Veterinaria. 2022;72(3):309-323. doi:10.2478/acve-2022-0025 .
Antić, Milutin, Beletić, Anđelo, Radaković, Milena, Spariosu, Kristina, Stanković, Sanja, Trailović, Dragiša, Kovačević Filipović, Milica, "Homocysteine concentration in the serum of dogs naturally infected with Leishmania spp. – association with the stage of the disease, therapy, and clinical pathology data" in Acta Veterinaria, 72, no. 3 (2022):309-323, https://doi.org/10.2478/acve-2022-0025 . .