Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination
Само за регистроване кориснике
2019
Аутори
Milovanović, MilovanMilićević, Vesna
Radojičić, Sonja
Valčić, Miroslav
Dietze, Klaas
Hoffmann, Bernd
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The commercially available ID Screen® Capripox double antigen ELISA from IDvet® validated and
authorized for serum and plasma, recently improved the options for assessing the humoral immune
response against lumpy skin disease (LSD). In this study, the suitability of milk (individual and bulk)
and colostrum as samples for this ELISA was investigated.
Samples (serum, milk and colostrum) were collected three times (before, one and five months post
re-vaccination) in LSDV-field strain free regions in Serbia. Test specificity was confirmed by
analyzing of 352 individual milk samples from Germany.
From 154 collected serum samples from Serbia, 75 were detected as positive by the Capripox
ELISA. Milk samples were tested using protocol for serum and with a modified protocol (increased
incubation time). Obtained results of milk and colostrum samples were analyzed with reduced cutoff value. Out of 154 milk samples, 38 samples were detected as positive using the protocol for
serum, where...as with modified protocol the number of positive sample increased up to 48. Only
two milk samples from Germany had border line results with the modified protocol. Sensitivity and
specificity of the ELISA for milk samples reached values in-between 88% to 91%, with significant
statistical difference (p<0.05) between both protocols. In addition, antibody detection was
possible in bulk milk and colostrum samples.
In principle, the commercially available Capripox ELISA, developed for antibody detection in serum
and plasma, shows to be suitable for detection of LSD-specific antibodies in milk samples and can
be a helpful tool in LSDV monitoring programs.
Извор:
13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019, 2019, 204-204Колекције
Институција/група
Fakultet veterinarske medicineTY - CONF AU - Milovanović, Milovan AU - Milićević, Vesna AU - Radojičić, Sonja AU - Valčić, Miroslav AU - Dietze, Klaas AU - Hoffmann, Bernd PY - 2019 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2753 AB - The commercially available ID Screen® Capripox double antigen ELISA from IDvet® validated and authorized for serum and plasma, recently improved the options for assessing the humoral immune response against lumpy skin disease (LSD). In this study, the suitability of milk (individual and bulk) and colostrum as samples for this ELISA was investigated. Samples (serum, milk and colostrum) were collected three times (before, one and five months post re-vaccination) in LSDV-field strain free regions in Serbia. Test specificity was confirmed by analyzing of 352 individual milk samples from Germany. From 154 collected serum samples from Serbia, 75 were detected as positive by the Capripox ELISA. Milk samples were tested using protocol for serum and with a modified protocol (increased incubation time). Obtained results of milk and colostrum samples were analyzed with reduced cutoff value. Out of 154 milk samples, 38 samples were detected as positive using the protocol for serum, whereas with modified protocol the number of positive sample increased up to 48. Only two milk samples from Germany had border line results with the modified protocol. Sensitivity and specificity of the ELISA for milk samples reached values in-between 88% to 91%, with significant statistical difference (p<0.05) between both protocols. In addition, antibody detection was possible in bulk milk and colostrum samples. In principle, the commercially available Capripox ELISA, developed for antibody detection in serum and plasma, shows to be suitable for detection of LSD-specific antibodies in milk samples and can be a helpful tool in LSDV monitoring programs. C3 - 13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019 T1 - Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination SP - 204 EP - 204 UR - https://hdl.handle.net/21.15107/rcub_veterinar_2753 ER -
@conference{ author = "Milovanović, Milovan and Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Dietze, Klaas and Hoffmann, Bernd", year = "2019", abstract = "The commercially available ID Screen® Capripox double antigen ELISA from IDvet® validated and authorized for serum and plasma, recently improved the options for assessing the humoral immune response against lumpy skin disease (LSD). In this study, the suitability of milk (individual and bulk) and colostrum as samples for this ELISA was investigated. Samples (serum, milk and colostrum) were collected three times (before, one and five months post re-vaccination) in LSDV-field strain free regions in Serbia. Test specificity was confirmed by analyzing of 352 individual milk samples from Germany. From 154 collected serum samples from Serbia, 75 were detected as positive by the Capripox ELISA. Milk samples were tested using protocol for serum and with a modified protocol (increased incubation time). Obtained results of milk and colostrum samples were analyzed with reduced cutoff value. Out of 154 milk samples, 38 samples were detected as positive using the protocol for serum, whereas with modified protocol the number of positive sample increased up to 48. Only two milk samples from Germany had border line results with the modified protocol. Sensitivity and specificity of the ELISA for milk samples reached values in-between 88% to 91%, with significant statistical difference (p<0.05) between both protocols. In addition, antibody detection was possible in bulk milk and colostrum samples. In principle, the commercially available Capripox ELISA, developed for antibody detection in serum and plasma, shows to be suitable for detection of LSD-specific antibodies in milk samples and can be a helpful tool in LSDV monitoring programs.", journal = "13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019", title = "Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination", pages = "204-204", url = "https://hdl.handle.net/21.15107/rcub_veterinar_2753" }
Milovanović, M., Milićević, V., Radojičić, S., Valčić, M., Dietze, K.,& Hoffmann, B.. (2019). Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination. in 13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019, 204-204. https://hdl.handle.net/21.15107/rcub_veterinar_2753
Milovanović M, Milićević V, Radojičić S, Valčić M, Dietze K, Hoffmann B. Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination. in 13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019. 2019;:204-204. https://hdl.handle.net/21.15107/rcub_veterinar_2753 .
Milovanović, Milovan, Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Dietze, Klaas, Hoffmann, Bernd, "Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination" in 13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019 (2019):204-204, https://hdl.handle.net/21.15107/rcub_veterinar_2753 .