Occurrence of Pseudomonas spp. in raw milk
Nalaz Pseudomonas spp. u sirovom mleku
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The genus Pseudomonas includes gram-negative bacilli, not requireing nutrient compounds necessary for growth and inhabit numerous ecological niches. They are often isolated from food, soil, manure and animal skin. They are classified into opportunistic pathogenic microorganisms because they can cause acute, or chronic infections of various mammalian hosts and their organs due to the ability to form a wide spectrum of virulence factors. Pseudomonas species grow well on bacterial media and often produce pigment, such as P. aeruginosa-thermostable blue-green pigment (pyocyanin) and P. flurescens-greenish-yellow pigment fluorescin. From the view point of milk hygiene the importance of this psychotrophic microorganism is, even it is destroyed by the pasteurization process, abbility to synthetize thermostable metabolic products and enzymes that cause spolage of milk products. The most commonly isolated is P. aeruginosa, but also P. flurescens, which leads to spolage of butter and UHT milk. T...he aim of this research was to point out the significance of Pseudomonas species as the milk contaminant and the possible cause of spolage.
The material represented seven bulk tank milk samples in witch the somatic cell counts were less than 400,000/ml. The isolation and identification was carried out during the routine testing of raw milk by the method ISO 6579-1: 2017. Colonies on XLD agar that were lactose negative with or without H2S, and with or without blue pigmantation around the colonies and characteristic smell were biochemically examined. The blood agar was used for the examination of haemolytic activity, and the casein agar and tributirin agar for the determination of proteolytic and lipolytic activity, respectively.
Results: Out of 7 milk samples Pseudomonas spp. was detected in 6 (85.71%) samples of raw milk. All 28 isolates were oxidase and catalase positive, they produced β hemolysis on blood agar, they produced a pigment on Tripton soy agar (TSA) and had a distinctive smell. All 28 isolates showed proteolytic and lipolytic activity. The most commonly isolated is Pseudomonas floroscens, and two Pseudomonas aeriginosa isolates produced green pigment-pyocyanin.
CONCLUSION: Although according the legislation in the Republic of Serbia does not provide the examination of milk samples for the presence of Pseudomonas species, it is possible to find this microorganism in raw milk as a result of contamination.
Rod Pseudomonas obuhvta gram-negativne štapiće, koji nisu zahtevni u pogledu hranjivih
sastojaka neophodnih za rast i naseljavaju brojne ekološke niše. Često su izolovani iz hrane,
zemlje, štalskog đubriva i sa kože životinja. Svrstavaju se u oportune patogene
mikroorganizme, jer mogu da izazovu akutne, ili hronične infekcije različitih domaćina-sisara
i njihovih organa zbog sposobnosti stvaranja širokog spektra faktora virulencije.
Pseudomonas vrste dobro rastu na bakteriološkim podlogama i često stvaraju pigment, i to
P. aeruginosa termostabilni plavo-zeleni pigment (pyocianin) i P. flurescens zelenkasto-žuti
pigment fluorescin. Značaj sa aspekta higijene mleka se ogleda u tome što, iako se ovaj
psihrotrofni mikroorganizam uništava procesom pasterizacije, može da stvara termostabilne
metaboličke produkte i enzime, koji izazvaju kvar proizvoda od mleka. Najčešće izolvan je P.
aeruginosa, ali i P. flurescens, koji dovodi do kvara maslaca i UHT mleka.
CILJ ovog rada je bio da... se ukaže na značaj Pseudomonas vrsta kao konatminenta mleka i
mogućeg uzročnika kvara.
Materijal je predstavljalo 7 uzoraka zbirnog mleka sa brojem somatskih ćelija manjim od
400.000/ml. Nalaz i identifikacija je rađena tokom rutinskog ispitivanja sirovog mleka
metodom ISO 6579-1:2017. Kolonije na XLD agaru koje su bile laktoza negativne sa ili bez
H2S, a sa, ili bez plavičaste boje oko kolonija i karakterističnim mirisom dalje su biohemijski
ispitivane. Krvni agar je korišćen za ispitivanje hemolitične aktivnosti, a podloga sa kazeinom
i tributirin agar i za ispitivanje proteolitičke i lipolitičke aktivnosti.
Rezultati: Od 7 uzoraka mleka Pseudomonas spp. je dokazan u 6 (85,71%) uzoraka sirovog
mleka. Svih 28 izolati su oksidaza i katalaza pozitivni, stvarali su β hemolizu na krvnom agaru,
proizvodili su pigment na Tripton soja agaru (TSA) i imali karakterističan miris. Svih 28
izolata je pokazalo proteolitičku i lipolitičku aktivnost. Najčešće izolovan je Pseudomonas
floroscens, a dva izolata Pseudomonas aeriginosa su stvarala zeleni pigment pyocianin.
ZAKLJUČAK: Iako zakonska regulativa u Republici Srbiji ne predviđa ispitivanje uzoraka
mleka na prisustvo Pseudomonas vrsta, moguć je nalaz ovog mikroorganizma u sirovom
mleku, kao posledica kontaminacije.
KLJUČNE REČI: mleko, Pseudomonas spp., kvar
Кључне речи:
milk / Pseudomonas spp. / spoilageИзвор:
XII Congress of Serbian Microbiologists with International Participation MICROMED 2018 REGIO, 2018Издавач:
- Beograd : Udruženje mikrobiologa Srbije
Напомена:
- Book of Abstracts
Колекције
Институција/група
Fakultet veterinarske medicineTY - CONF AU - Savić Radovanović, Radoslava PY - 2018 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2764 AB - The genus Pseudomonas includes gram-negative bacilli, not requireing nutrient compounds necessary for growth and inhabit numerous ecological niches. They are often isolated from food, soil, manure and animal skin. They are classified into opportunistic pathogenic microorganisms because they can cause acute, or chronic infections of various mammalian hosts and their organs due to the ability to form a wide spectrum of virulence factors. Pseudomonas species grow well on bacterial media and often produce pigment, such as P. aeruginosa-thermostable blue-green pigment (pyocyanin) and P. flurescens-greenish-yellow pigment fluorescin. From the view point of milk hygiene the importance of this psychotrophic microorganism is, even it is destroyed by the pasteurization process, abbility to synthetize thermostable metabolic products and enzymes that cause spolage of milk products. The most commonly isolated is P. aeruginosa, but also P. flurescens, which leads to spolage of butter and UHT milk. The aim of this research was to point out the significance of Pseudomonas species as the milk contaminant and the possible cause of spolage. The material represented seven bulk tank milk samples in witch the somatic cell counts were less than 400,000/ml. The isolation and identification was carried out during the routine testing of raw milk by the method ISO 6579-1: 2017. Colonies on XLD agar that were lactose negative with or without H2S, and with or without blue pigmantation around the colonies and characteristic smell were biochemically examined. The blood agar was used for the examination of haemolytic activity, and the casein agar and tributirin agar for the determination of proteolytic and lipolytic activity, respectively. Results: Out of 7 milk samples Pseudomonas spp. was detected in 6 (85.71%) samples of raw milk. All 28 isolates were oxidase and catalase positive, they produced β hemolysis on blood agar, they produced a pigment on Tripton soy agar (TSA) and had a distinctive smell. All 28 isolates showed proteolytic and lipolytic activity. The most commonly isolated is Pseudomonas floroscens, and two Pseudomonas aeriginosa isolates produced green pigment-pyocyanin. CONCLUSION: Although according the legislation in the Republic of Serbia does not provide the examination of milk samples for the presence of Pseudomonas species, it is possible to find this microorganism in raw milk as a result of contamination. AB - Rod Pseudomonas obuhvta gram-negativne štapiće, koji nisu zahtevni u pogledu hranjivih sastojaka neophodnih za rast i naseljavaju brojne ekološke niše. Često su izolovani iz hrane, zemlje, štalskog đubriva i sa kože životinja. Svrstavaju se u oportune patogene mikroorganizme, jer mogu da izazovu akutne, ili hronične infekcije različitih domaćina-sisara i njihovih organa zbog sposobnosti stvaranja širokog spektra faktora virulencije. Pseudomonas vrste dobro rastu na bakteriološkim podlogama i često stvaraju pigment, i to P. aeruginosa termostabilni plavo-zeleni pigment (pyocianin) i P. flurescens zelenkasto-žuti pigment fluorescin. Značaj sa aspekta higijene mleka se ogleda u tome što, iako se ovaj psihrotrofni mikroorganizam uništava procesom pasterizacije, može da stvara termostabilne metaboličke produkte i enzime, koji izazvaju kvar proizvoda od mleka. Najčešće izolvan je P. aeruginosa, ali i P. flurescens, koji dovodi do kvara maslaca i UHT mleka. CILJ ovog rada je bio da se ukaže na značaj Pseudomonas vrsta kao konatminenta mleka i mogućeg uzročnika kvara. Materijal je predstavljalo 7 uzoraka zbirnog mleka sa brojem somatskih ćelija manjim od 400.000/ml. Nalaz i identifikacija je rađena tokom rutinskog ispitivanja sirovog mleka metodom ISO 6579-1:2017. Kolonije na XLD agaru koje su bile laktoza negativne sa ili bez H2S, a sa, ili bez plavičaste boje oko kolonija i karakterističnim mirisom dalje su biohemijski ispitivane. Krvni agar je korišćen za ispitivanje hemolitične aktivnosti, a podloga sa kazeinom i tributirin agar i za ispitivanje proteolitičke i lipolitičke aktivnosti. Rezultati: Od 7 uzoraka mleka Pseudomonas spp. je dokazan u 6 (85,71%) uzoraka sirovog mleka. Svih 28 izolati su oksidaza i katalaza pozitivni, stvarali su β hemolizu na krvnom agaru, proizvodili su pigment na Tripton soja agaru (TSA) i imali karakterističan miris. Svih 28 izolata je pokazalo proteolitičku i lipolitičku aktivnost. Najčešće izolovan je Pseudomonas floroscens, a dva izolata Pseudomonas aeriginosa su stvarala zeleni pigment pyocianin. ZAKLJUČAK: Iako zakonska regulativa u Republici Srbiji ne predviđa ispitivanje uzoraka mleka na prisustvo Pseudomonas vrsta, moguć je nalaz ovog mikroorganizma u sirovom mleku, kao posledica kontaminacije. KLJUČNE REČI: mleko, Pseudomonas spp., kvar PB - Beograd : Udruženje mikrobiologa Srbije C3 - XII Congress of Serbian Microbiologists with International Participation MICROMED 2018 REGIO T1 - Occurrence of Pseudomonas spp. in raw milk T1 - Nalaz Pseudomonas spp. u sirovom mleku UR - https://hdl.handle.net/21.15107/rcub_veterinar_2764 ER -
@conference{ author = "Savić Radovanović, Radoslava", year = "2018", abstract = "The genus Pseudomonas includes gram-negative bacilli, not requireing nutrient compounds necessary for growth and inhabit numerous ecological niches. They are often isolated from food, soil, manure and animal skin. They are classified into opportunistic pathogenic microorganisms because they can cause acute, or chronic infections of various mammalian hosts and their organs due to the ability to form a wide spectrum of virulence factors. Pseudomonas species grow well on bacterial media and often produce pigment, such as P. aeruginosa-thermostable blue-green pigment (pyocyanin) and P. flurescens-greenish-yellow pigment fluorescin. From the view point of milk hygiene the importance of this psychotrophic microorganism is, even it is destroyed by the pasteurization process, abbility to synthetize thermostable metabolic products and enzymes that cause spolage of milk products. The most commonly isolated is P. aeruginosa, but also P. flurescens, which leads to spolage of butter and UHT milk. The aim of this research was to point out the significance of Pseudomonas species as the milk contaminant and the possible cause of spolage. The material represented seven bulk tank milk samples in witch the somatic cell counts were less than 400,000/ml. The isolation and identification was carried out during the routine testing of raw milk by the method ISO 6579-1: 2017. Colonies on XLD agar that were lactose negative with or without H2S, and with or without blue pigmantation around the colonies and characteristic smell were biochemically examined. The blood agar was used for the examination of haemolytic activity, and the casein agar and tributirin agar for the determination of proteolytic and lipolytic activity, respectively. Results: Out of 7 milk samples Pseudomonas spp. was detected in 6 (85.71%) samples of raw milk. All 28 isolates were oxidase and catalase positive, they produced β hemolysis on blood agar, they produced a pigment on Tripton soy agar (TSA) and had a distinctive smell. All 28 isolates showed proteolytic and lipolytic activity. The most commonly isolated is Pseudomonas floroscens, and two Pseudomonas aeriginosa isolates produced green pigment-pyocyanin. CONCLUSION: Although according the legislation in the Republic of Serbia does not provide the examination of milk samples for the presence of Pseudomonas species, it is possible to find this microorganism in raw milk as a result of contamination., Rod Pseudomonas obuhvta gram-negativne štapiće, koji nisu zahtevni u pogledu hranjivih sastojaka neophodnih za rast i naseljavaju brojne ekološke niše. Često su izolovani iz hrane, zemlje, štalskog đubriva i sa kože životinja. Svrstavaju se u oportune patogene mikroorganizme, jer mogu da izazovu akutne, ili hronične infekcije različitih domaćina-sisara i njihovih organa zbog sposobnosti stvaranja širokog spektra faktora virulencije. Pseudomonas vrste dobro rastu na bakteriološkim podlogama i često stvaraju pigment, i to P. aeruginosa termostabilni plavo-zeleni pigment (pyocianin) i P. flurescens zelenkasto-žuti pigment fluorescin. Značaj sa aspekta higijene mleka se ogleda u tome što, iako se ovaj psihrotrofni mikroorganizam uništava procesom pasterizacije, može da stvara termostabilne metaboličke produkte i enzime, koji izazvaju kvar proizvoda od mleka. Najčešće izolvan je P. aeruginosa, ali i P. flurescens, koji dovodi do kvara maslaca i UHT mleka. CILJ ovog rada je bio da se ukaže na značaj Pseudomonas vrsta kao konatminenta mleka i mogućeg uzročnika kvara. Materijal je predstavljalo 7 uzoraka zbirnog mleka sa brojem somatskih ćelija manjim od 400.000/ml. Nalaz i identifikacija je rađena tokom rutinskog ispitivanja sirovog mleka metodom ISO 6579-1:2017. Kolonije na XLD agaru koje su bile laktoza negativne sa ili bez H2S, a sa, ili bez plavičaste boje oko kolonija i karakterističnim mirisom dalje su biohemijski ispitivane. Krvni agar je korišćen za ispitivanje hemolitične aktivnosti, a podloga sa kazeinom i tributirin agar i za ispitivanje proteolitičke i lipolitičke aktivnosti. Rezultati: Od 7 uzoraka mleka Pseudomonas spp. je dokazan u 6 (85,71%) uzoraka sirovog mleka. Svih 28 izolati su oksidaza i katalaza pozitivni, stvarali su β hemolizu na krvnom agaru, proizvodili su pigment na Tripton soja agaru (TSA) i imali karakterističan miris. Svih 28 izolata je pokazalo proteolitičku i lipolitičku aktivnost. Najčešće izolovan je Pseudomonas floroscens, a dva izolata Pseudomonas aeriginosa su stvarala zeleni pigment pyocianin. ZAKLJUČAK: Iako zakonska regulativa u Republici Srbiji ne predviđa ispitivanje uzoraka mleka na prisustvo Pseudomonas vrsta, moguć je nalaz ovog mikroorganizma u sirovom mleku, kao posledica kontaminacije. KLJUČNE REČI: mleko, Pseudomonas spp., kvar", publisher = "Beograd : Udruženje mikrobiologa Srbije", journal = "XII Congress of Serbian Microbiologists with International Participation MICROMED 2018 REGIO", title = "Occurrence of Pseudomonas spp. in raw milk, Nalaz Pseudomonas spp. u sirovom mleku", url = "https://hdl.handle.net/21.15107/rcub_veterinar_2764" }
Savić Radovanović, R.. (2018). Occurrence of Pseudomonas spp. in raw milk. in XII Congress of Serbian Microbiologists with International Participation MICROMED 2018 REGIO Beograd : Udruženje mikrobiologa Srbije.. https://hdl.handle.net/21.15107/rcub_veterinar_2764
Savić Radovanović R. Occurrence of Pseudomonas spp. in raw milk. in XII Congress of Serbian Microbiologists with International Participation MICROMED 2018 REGIO. 2018;. https://hdl.handle.net/21.15107/rcub_veterinar_2764 .
Savić Radovanović, Radoslava, "Occurrence of Pseudomonas spp. in raw milk" in XII Congress of Serbian Microbiologists with International Participation MICROMED 2018 REGIO (2018), https://hdl.handle.net/21.15107/rcub_veterinar_2764 .