Cutaneous basophil hypersensitivity reaction to phytohemagglutinin in repeat breeder cows

Abstract

In this study the relationship was explored between general immune reactivity, as estimated by the cutaneous basophil hypersensitivity reaction (CBHR) to phytohemagglutinin (PHA), and the level of anti-sperm antibodies of the Ig A and Ig G class in the cervical mucus and sera of cows with different reproductive results. The hypersensitivity test was performed on the day of artificial insemination when cervical mucus and sera samples were also collected. The animals were divided into groups according to the total number of inseminations, number of inseminations per calving and their age. The titer of antibodies was determined by the indirect immunofluorescence method using sperm cells suspended in Tris egg-yolk extender. Our results indicate that the intensity of CBHR is lowest in cows inseminated once or twice and in younger animals, while, there were no differences between the groups when they were formed according to the number of inseminations per calving. Titers of antisperm antibodies (ASA) of the Ig A class in cervical mucus (CM) increased with the number of artificial inseminations, number of inseminations per calving and age of the cows.

Cilj ovih ispitivanja je bio da se utvrdi odnos između rezultata testa kožne preosetljivosti na fitohemaglutinin, nekih reproduktivnih pokazatelja i koncentracije antispermatozoalnih antitela Ig A klase u cervikalnoj sluzi krava koje se veštački osemenjavaju. Test kožne preosetljivosti je izvođen na dan veštačkog osemenjavanja a istog dana su prikupljani i uzorci cervikalne sluzi. Krave su bile podeljene u grupe na osnovu broja ukupnih prethodnih osemenjavanja, prosečnog broja osemenjavanja po graviditetu i na osnovu starosti. Prisustvo antispermatozoalnih antitela je određivano metodom indirektne imunofluorescence korišćenjem spermatozoida suspendovanih u Tris-žumanjčanom razređivaču. Postignuti rezultati ukazuju da je intenzitet kožne preosetljivosti na PHA manji kod krava koje su osemenjene 1-2 puta kao i kod mlađih jedinki. Intenzitet ove reakcije se nije razlikovao kod krava koje zahtevaju manje ili više pokušaja VO po teljenju. Takođe je dokazano da se titar antispermatozoalnih Ig A antitela u cervikalnoj sluzi povećava sa ukupnim brojem osemenjavanja, brojem osemenjavanja po teljenju i starošću plotkinja.