Alkaline phosphatase activity and isoforms in canine and feline effusions –A pilot study
Само за регистроване кориснике
2020
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Background: Bone, liver and corticosteroid‐induced ALP isoforms
are present in dog serum; liver isoform is present in cat serum.
Neoplastic cells can produce atypical ALP isoforms.
Objective: We hypothesized that ALP activity and isoforms in canine
and feline effusions might reflect the dominant pathogenesis of fluid
accumulation.
Methods: Dog (n = 9) and cat (n = 7) peritoneal/pleural effusions
were analyzed for ALP isoforms using zymography. Results of rou‐
tine effusion analysis were obtained from the laboratory (Vetlab,
Belgrade) database.
Results: Effusions were classified as modified transudate (MT) (n = 4),
exudate (n = 3), neoplastic (n = 4), feline infectious peritonitis (n = 3),
and chylous (n = 1). Two ALP isoforms were detected: type‐1 and
type‐2. In dogs, median ALP activity was higher in MTs (118 U/L,
range 61‐860 U/L) compared to exudates (22 U/L, 16‐116 U/L), and
neoplastic effusions (14 U/L, 10‐22 U/L) (Kruskall‐Wallis, P = .067); 3
MTs had type‐1 isofo...rm and one had both ALP isoforms. Neoplastic
effusions had variable patterns: no detectable isoforms (n = 2), type‐1
isoform (n = 1), and both isoforms (n = 1). In cats, an exudate with both
isoforms had the highest ALP activity, the chylous effusion had type‐2
isoform, and FIP effusions had undetectable ALP activity. Overall ALP
activity was not correlated with effusion TP concentration or TNCC.
Conclusions: Different patterns of ALP isoforms in effusions are
observed in canine and feline effusions that might indicate under‐
lying pathogenesis. Future studies are needed to determine spe‐
cific ALP isoforms in effusions that result from known underlying
diseases.
Извор:
The American Society of Veterinary Clinical Pathology (ASVCP) 55th Annual Meeting in conjunction with the American College of Veterinary Pathology (ACVP) 71th Annual Meeting, and the International Society of Animal Clinical Pathology (ISACP) 19th Biannual Meeting VIRTUAL–October 30‐November 1, 2020, 2020, 116-116Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
Колекције
Институција/група
Fakultet veterinarske medicineTY - CONF AU - Spariosu, Kristina AU - Medić, Strahinja AU - Radaković, Milena AU - Kovačević Filipović, Milica PY - 2020 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2793 AB - Background: Bone, liver and corticosteroid‐induced ALP isoforms are present in dog serum; liver isoform is present in cat serum. Neoplastic cells can produce atypical ALP isoforms. Objective: We hypothesized that ALP activity and isoforms in canine and feline effusions might reflect the dominant pathogenesis of fluid accumulation. Methods: Dog (n = 9) and cat (n = 7) peritoneal/pleural effusions were analyzed for ALP isoforms using zymography. Results of rou‐ tine effusion analysis were obtained from the laboratory (Vetlab, Belgrade) database. Results: Effusions were classified as modified transudate (MT) (n = 4), exudate (n = 3), neoplastic (n = 4), feline infectious peritonitis (n = 3), and chylous (n = 1). Two ALP isoforms were detected: type‐1 and type‐2. In dogs, median ALP activity was higher in MTs (118 U/L, range 61‐860 U/L) compared to exudates (22 U/L, 16‐116 U/L), and neoplastic effusions (14 U/L, 10‐22 U/L) (Kruskall‐Wallis, P = .067); 3 MTs had type‐1 isoform and one had both ALP isoforms. Neoplastic effusions had variable patterns: no detectable isoforms (n = 2), type‐1 isoform (n = 1), and both isoforms (n = 1). In cats, an exudate with both isoforms had the highest ALP activity, the chylous effusion had type‐2 isoform, and FIP effusions had undetectable ALP activity. Overall ALP activity was not correlated with effusion TP concentration or TNCC. Conclusions: Different patterns of ALP isoforms in effusions are observed in canine and feline effusions that might indicate under‐ lying pathogenesis. Future studies are needed to determine spe‐ cific ALP isoforms in effusions that result from known underlying diseases. C3 - The American Society of Veterinary Clinical Pathology (ASVCP) 55th Annual Meeting in conjunction with the American College of Veterinary Pathology (ACVP) 71th Annual Meeting, and the International Society of Animal Clinical Pathology (ISACP) 19th Biannual Meeting VIRTUAL–October 30‐November 1, 2020 T1 - Alkaline phosphatase activity and isoforms in canine and feline effusions –A pilot study SP - 116 EP - 116 UR - https://hdl.handle.net/21.15107/rcub_veterinar_2793 ER -
@conference{ author = "Spariosu, Kristina and Medić, Strahinja and Radaković, Milena and Kovačević Filipović, Milica", year = "2020", abstract = "Background: Bone, liver and corticosteroid‐induced ALP isoforms are present in dog serum; liver isoform is present in cat serum. Neoplastic cells can produce atypical ALP isoforms. Objective: We hypothesized that ALP activity and isoforms in canine and feline effusions might reflect the dominant pathogenesis of fluid accumulation. Methods: Dog (n = 9) and cat (n = 7) peritoneal/pleural effusions were analyzed for ALP isoforms using zymography. Results of rou‐ tine effusion analysis were obtained from the laboratory (Vetlab, Belgrade) database. Results: Effusions were classified as modified transudate (MT) (n = 4), exudate (n = 3), neoplastic (n = 4), feline infectious peritonitis (n = 3), and chylous (n = 1). Two ALP isoforms were detected: type‐1 and type‐2. In dogs, median ALP activity was higher in MTs (118 U/L, range 61‐860 U/L) compared to exudates (22 U/L, 16‐116 U/L), and neoplastic effusions (14 U/L, 10‐22 U/L) (Kruskall‐Wallis, P = .067); 3 MTs had type‐1 isoform and one had both ALP isoforms. Neoplastic effusions had variable patterns: no detectable isoforms (n = 2), type‐1 isoform (n = 1), and both isoforms (n = 1). In cats, an exudate with both isoforms had the highest ALP activity, the chylous effusion had type‐2 isoform, and FIP effusions had undetectable ALP activity. Overall ALP activity was not correlated with effusion TP concentration or TNCC. Conclusions: Different patterns of ALP isoforms in effusions are observed in canine and feline effusions that might indicate under‐ lying pathogenesis. Future studies are needed to determine spe‐ cific ALP isoforms in effusions that result from known underlying diseases.", journal = "The American Society of Veterinary Clinical Pathology (ASVCP) 55th Annual Meeting in conjunction with the American College of Veterinary Pathology (ACVP) 71th Annual Meeting, and the International Society of Animal Clinical Pathology (ISACP) 19th Biannual Meeting VIRTUAL–October 30‐November 1, 2020", title = "Alkaline phosphatase activity and isoforms in canine and feline effusions –A pilot study", pages = "116-116", url = "https://hdl.handle.net/21.15107/rcub_veterinar_2793" }
Spariosu, K., Medić, S., Radaković, M.,& Kovačević Filipović, M.. (2020). Alkaline phosphatase activity and isoforms in canine and feline effusions –A pilot study. in The American Society of Veterinary Clinical Pathology (ASVCP) 55th Annual Meeting in conjunction with the American College of Veterinary Pathology (ACVP) 71th Annual Meeting, and the International Society of Animal Clinical Pathology (ISACP) 19th Biannual Meeting VIRTUAL–October 30‐November 1, 2020, 116-116. https://hdl.handle.net/21.15107/rcub_veterinar_2793
Spariosu K, Medić S, Radaković M, Kovačević Filipović M. Alkaline phosphatase activity and isoforms in canine and feline effusions –A pilot study. in The American Society of Veterinary Clinical Pathology (ASVCP) 55th Annual Meeting in conjunction with the American College of Veterinary Pathology (ACVP) 71th Annual Meeting, and the International Society of Animal Clinical Pathology (ISACP) 19th Biannual Meeting VIRTUAL–October 30‐November 1, 2020. 2020;:116-116. https://hdl.handle.net/21.15107/rcub_veterinar_2793 .
Spariosu, Kristina, Medić, Strahinja, Radaković, Milena, Kovačević Filipović, Milica, "Alkaline phosphatase activity and isoforms in canine and feline effusions –A pilot study" in The American Society of Veterinary Clinical Pathology (ASVCP) 55th Annual Meeting in conjunction with the American College of Veterinary Pathology (ACVP) 71th Annual Meeting, and the International Society of Animal Clinical Pathology (ISACP) 19th Biannual Meeting VIRTUAL–October 30‐November 1, 2020 (2020):116-116, https://hdl.handle.net/21.15107/rcub_veterinar_2793 .