Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity
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2022
Authors
Kostić, SanjaVilotić, Aleksandra
Pirković, Andrea
Dekanski, Dragana
Borozan, Sunčica
Nacka-Aleksić, Mirjana
Vrzić-Petronijević, Svetlana
Jovanović Krivokuća, Milica
Article (Published version)
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Caffeic acid is highlighted as one of the major phenolic compounds present in foods with known antioxidant activity. This phenolic is among commonly consumed substances in everyday diet of pregnant women. However, there is not enough information on its effects during pregnancy, especially the most vulnerable early stage. Extravillous trophoblast cells are specific cells of the placenta that come in direct contact with maternal uterine tissue. Through this study we investigated the cytoprotective effects of caffeic acid on H2O2-induced oxidative damage in first trimester extravillous trophoblast cell line HTR-8/SVneo. Investigated concentrations (1–100 μM) of caffeic acid showed neither cytotoxic nor genotoxic effects on HTR-8/SVneo cells. The treatment with caffeic acid 100 μM significantly increased the percentage of cells in G2/M phase of the cell cycle, compared to non-treated cells. Pretreatment with caffeic acid (10 and 100 μM) attenuated oxidative DNA damage significantly, reduce...d cytotoxicity, protein and lipid peroxidation, and restored antioxidant capacity in trophoblast cells following H2O2 exposure. This beneficial outcome is probably mediated by the augmentation of GSH and effective ROS scavenging by caffeic acid. These promising results require further investigations to reveal the additional mechanisms/pathways and confirmation through studies in vivo.
Keywords:
Caffeic acid / HTR-8/SVneo cells / Cytotoxicity / Oxidative DNA damageSource:
Food and Chemical Toxicology, 2022, 163, 112993-Publisher:
- Elsevier
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200019 (University of Belgrade, Institute for the Application of Nuclear Energy - INEP) (RS-MESTD-inst-2020-200019)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200143 (University of Belgrade, Faculty of Veterinary Medicine) (RS-MESTD-inst-2020-200143)
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Fakultet veterinarske medicineTY - JOUR AU - Kostić, Sanja AU - Vilotić, Aleksandra AU - Pirković, Andrea AU - Dekanski, Dragana AU - Borozan, Sunčica AU - Nacka-Aleksić, Mirjana AU - Vrzić-Petronijević, Svetlana AU - Jovanović Krivokuća, Milica PY - 2022 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2842 AB - Caffeic acid is highlighted as one of the major phenolic compounds present in foods with known antioxidant activity. This phenolic is among commonly consumed substances in everyday diet of pregnant women. However, there is not enough information on its effects during pregnancy, especially the most vulnerable early stage. Extravillous trophoblast cells are specific cells of the placenta that come in direct contact with maternal uterine tissue. Through this study we investigated the cytoprotective effects of caffeic acid on H2O2-induced oxidative damage in first trimester extravillous trophoblast cell line HTR-8/SVneo. Investigated concentrations (1–100 μM) of caffeic acid showed neither cytotoxic nor genotoxic effects on HTR-8/SVneo cells. The treatment with caffeic acid 100 μM significantly increased the percentage of cells in G2/M phase of the cell cycle, compared to non-treated cells. Pretreatment with caffeic acid (10 and 100 μM) attenuated oxidative DNA damage significantly, reduced cytotoxicity, protein and lipid peroxidation, and restored antioxidant capacity in trophoblast cells following H2O2 exposure. This beneficial outcome is probably mediated by the augmentation of GSH and effective ROS scavenging by caffeic acid. These promising results require further investigations to reveal the additional mechanisms/pathways and confirmation through studies in vivo. PB - Elsevier T2 - Food and Chemical Toxicology T1 - Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity VL - 163 SP - 112993 DO - 10.1016/j.fct.2022.112993 ER -
@article{ author = "Kostić, Sanja and Vilotić, Aleksandra and Pirković, Andrea and Dekanski, Dragana and Borozan, Sunčica and Nacka-Aleksić, Mirjana and Vrzić-Petronijević, Svetlana and Jovanović Krivokuća, Milica", year = "2022", abstract = "Caffeic acid is highlighted as one of the major phenolic compounds present in foods with known antioxidant activity. This phenolic is among commonly consumed substances in everyday diet of pregnant women. However, there is not enough information on its effects during pregnancy, especially the most vulnerable early stage. Extravillous trophoblast cells are specific cells of the placenta that come in direct contact with maternal uterine tissue. Through this study we investigated the cytoprotective effects of caffeic acid on H2O2-induced oxidative damage in first trimester extravillous trophoblast cell line HTR-8/SVneo. Investigated concentrations (1–100 μM) of caffeic acid showed neither cytotoxic nor genotoxic effects on HTR-8/SVneo cells. The treatment with caffeic acid 100 μM significantly increased the percentage of cells in G2/M phase of the cell cycle, compared to non-treated cells. Pretreatment with caffeic acid (10 and 100 μM) attenuated oxidative DNA damage significantly, reduced cytotoxicity, protein and lipid peroxidation, and restored antioxidant capacity in trophoblast cells following H2O2 exposure. This beneficial outcome is probably mediated by the augmentation of GSH and effective ROS scavenging by caffeic acid. These promising results require further investigations to reveal the additional mechanisms/pathways and confirmation through studies in vivo.", publisher = "Elsevier", journal = "Food and Chemical Toxicology", title = "Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity", volume = "163", pages = "112993", doi = "10.1016/j.fct.2022.112993" }
Kostić, S., Vilotić, A., Pirković, A., Dekanski, D., Borozan, S., Nacka-Aleksić, M., Vrzić-Petronijević, S.,& Jovanović Krivokuća, M.. (2022). Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity. in Food and Chemical Toxicology Elsevier., 163, 112993. https://doi.org/10.1016/j.fct.2022.112993
Kostić S, Vilotić A, Pirković A, Dekanski D, Borozan S, Nacka-Aleksić M, Vrzić-Petronijević S, Jovanović Krivokuća M. Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity. in Food and Chemical Toxicology. 2022;163:112993. doi:10.1016/j.fct.2022.112993 .
Kostić, Sanja, Vilotić, Aleksandra, Pirković, Andrea, Dekanski, Dragana, Borozan, Sunčica, Nacka-Aleksić, Mirjana, Vrzić-Petronijević, Svetlana, Jovanović Krivokuća, Milica, "Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity" in Food and Chemical Toxicology, 163 (2022):112993, https://doi.org/10.1016/j.fct.2022.112993 . .