Mastitis krava prouzrokovan sa Pseudomonas spp.

Abstract

Mastitis je zapaljenski proces u mlečnoj žlezdi muznih životinja, koji je najčešće prouzrokovan mikroorganizmima. Po toku mastitisi mogu biti akutni, subakutni i hronični, a po obliku klinički i subkunički. Sa aspekta higijene mleka značaj imaju subklinički mastitisi, jer se ne manifestuju vidljivim kliničkim simptomima, a dijagnoza se postavlja pregledom mleka. Mastitisi imaju višestruki značaj (higijensko-zdravstveni, tehnološki i ekonomski). Uzročnici kontagioznih mastitisa su Staphylococcus aureus, Streptococcus agalatiae i Mycoplasma spp. Kao uzročnici iz okoline se navode koliformni mikroorganizmi (E.coli, Klebsiella spp., Enterobacter spp.), Streptococcus spp i Pseudomonas spp. Rod Peudomonas obuhvata gram-negativne štapiće rasprostranjene u vodi i spoljašnjoj sredini, koji mogu da izazovu mastitise i u klinički izraženom obliku. Obično se javljaju sporadično, ali mogu da izazovu ozbiljne probleme u stadu. Na krvnom agaru rastu u vidu krupnih, nepravilnih kolonija sivkaste boje, koje imaju tendenciju prerastanja podloge sa jasno izraženim tamnim centrom i providnom ivicom. Cilj ovog rada je bio da se postavi dijagnoza subkliničkog mastitisa, odredi antibiogram i na osnovu toga pravilno sprovede lečenje. Materijal su bili uzorci mleka uzeti aseptično iz četvrti krave kod koje su redovnom kontrolom-predmuznom probom, a potom i CMT testom utvđene promene u mleku. Zasejavanje uzoraka je izvršeno na krvni agar, koji je inkubisan pri 37°C 24-48h. Dijagnoza je postavljena na osnovu bakteriološkog pregleda i broja somatskih ćelija.Rezultati antibiograma su pokazali da je izolat Pseudomonas spp. osetljiv na: Excenel/Naxcel, Cephalonium, Tetracycline, Linkomicin i Sinulox. Terapija je uspešno primenjena aplikovanjem antibiotika intramamarno i parenteralno.

Mastitis is an inflammation of the mammary gland of dairy animals, which is usually caused by microorganisms. According to the stage mastitis can be acute, subacute and chronic and according to the form clinical and subclinical. Mastitis is of multiple importance (hygienic- health, technological and economic). From the view point of milk hygine subclinical mastitis is important because it does not manifest by visible clinical symptoms, and can be diagnosed by examination of the milk. Contagious mastitis pathogens are Staphylococcus aureus, Streptococcus agalatiae and Mycoplasma spp. Agents from the environment referred coliform microorganisms (E. coli, Klebsiella spp., Enterobacter spp.), Streptococcus spp. and Pseudomonas spp. The genus Peudomonas includes gram-negative bacilli originating from water and environment, which may cause mastitis in clinically expressed form. Usually occurs sporadically, but can cause serious problems in the herd. On the blood agar grows as large, irregular colonies of gray color, which tend to overgrow, with a clear dark center and transparent edge. The aim of this study was to diagnose subclinical mastitis in cow, detremine the antibiogram and carry out the proper therapy treatment. Material represented milk samples aseptically taken from the quarters in which the regular-control, by foremilk stripping and California Mastitis test showed changes in the milk. The samples were plated on blood agar plate, incubated at 37°C for 24-48 hours. The diagnosis was based on bacteriological examination and somatic cell count.The results showed that isolate Pseudomonas spp. was sensitive to Excenel / Naxcel, Cephalonium, Tetracycline, Lincomycin and Sinulox. The therapy was successfully applied by intramammary and parenteral administration of antibiotics.