Effects of selected essential oils on Listeria monocytogenes in biofilms and in a model food system
Аутори
Vidaković Knežević, SuzanaKnežević, Slobodan
Vranešević, Jelena
Kravić, Snežana Ž.
Lakićević, Brankica
Kocić-Tanackov, Sunčica
Karabasil, Nedjeljko
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The composition of 18 essential oils was determined using gas chromatography–mass spectrometry, and their antilisterial activity was evaluated by the disk diffusion method, followed by the determination of the minimum inhibitory and minimum bactericidal concentrations. The most active essential oils were oregano, thyme, cinnamon, winter savory, and clove, with MIC values ranging from 0.09 to 1.78 µL/mL. We investigated the biofilm-forming potential of Listeria monocytogenes on polystyrene at 5 °C, 15 °C, and 37 °C in three different media. The formation of biofilm was found to be dependent on the temperature and the availability of nutrients. After treatment with selected essential oils, the reduction in biofilm biomass was in the range of 32.61% and 78.62%. Micromorphological changes in the L. monocytogenes treated by oregano and thyme essential oils were observed in the form of impaired cell integrity and cell lyses by using scanning electron microscope. Oregano and thyme essential o...ils (MIC and 2MIC) significantly (p < 0.05) reduced the population of L. monocytogenes in minced pork meat during storage at 4 °C. In conclusion, the obtained results indicated the good activity of some selected essential oils on L. monocytogenes, with bacteriostatic, bactericidal, and antibiofilm effects at very low concentrations.
Кључне речи:
antimicrobial activity / antibiofilm activity / minced pork meat / Listeria monocytogenes / oregano essential oil / thyme essential oilИзвор:
Foods, 2023, 12, 10, 1930-Издавач:
- MDPI
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Vidaković Knežević, Suzana AU - Knežević, Slobodan AU - Vranešević, Jelena AU - Kravić, Snežana Ž. AU - Lakićević, Brankica AU - Kocić-Tanackov, Sunčica AU - Karabasil, Nedjeljko PY - 2023 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2940 AB - The composition of 18 essential oils was determined using gas chromatography–mass spectrometry, and their antilisterial activity was evaluated by the disk diffusion method, followed by the determination of the minimum inhibitory and minimum bactericidal concentrations. The most active essential oils were oregano, thyme, cinnamon, winter savory, and clove, with MIC values ranging from 0.09 to 1.78 µL/mL. We investigated the biofilm-forming potential of Listeria monocytogenes on polystyrene at 5 °C, 15 °C, and 37 °C in three different media. The formation of biofilm was found to be dependent on the temperature and the availability of nutrients. After treatment with selected essential oils, the reduction in biofilm biomass was in the range of 32.61% and 78.62%. Micromorphological changes in the L. monocytogenes treated by oregano and thyme essential oils were observed in the form of impaired cell integrity and cell lyses by using scanning electron microscope. Oregano and thyme essential oils (MIC and 2MIC) significantly (p < 0.05) reduced the population of L. monocytogenes in minced pork meat during storage at 4 °C. In conclusion, the obtained results indicated the good activity of some selected essential oils on L. monocytogenes, with bacteriostatic, bactericidal, and antibiofilm effects at very low concentrations. PB - MDPI T2 - Foods T1 - Effects of selected essential oils on Listeria monocytogenes in biofilms and in a model food system VL - 12 IS - 10 SP - 1930 DO - 10.3390/foods12101930 ER -
@article{ author = "Vidaković Knežević, Suzana and Knežević, Slobodan and Vranešević, Jelena and Kravić, Snežana Ž. and Lakićević, Brankica and Kocić-Tanackov, Sunčica and Karabasil, Nedjeljko", year = "2023", abstract = "The composition of 18 essential oils was determined using gas chromatography–mass spectrometry, and their antilisterial activity was evaluated by the disk diffusion method, followed by the determination of the minimum inhibitory and minimum bactericidal concentrations. The most active essential oils were oregano, thyme, cinnamon, winter savory, and clove, with MIC values ranging from 0.09 to 1.78 µL/mL. We investigated the biofilm-forming potential of Listeria monocytogenes on polystyrene at 5 °C, 15 °C, and 37 °C in three different media. The formation of biofilm was found to be dependent on the temperature and the availability of nutrients. After treatment with selected essential oils, the reduction in biofilm biomass was in the range of 32.61% and 78.62%. Micromorphological changes in the L. monocytogenes treated by oregano and thyme essential oils were observed in the form of impaired cell integrity and cell lyses by using scanning electron microscope. Oregano and thyme essential oils (MIC and 2MIC) significantly (p < 0.05) reduced the population of L. monocytogenes in minced pork meat during storage at 4 °C. In conclusion, the obtained results indicated the good activity of some selected essential oils on L. monocytogenes, with bacteriostatic, bactericidal, and antibiofilm effects at very low concentrations.", publisher = "MDPI", journal = "Foods", title = "Effects of selected essential oils on Listeria monocytogenes in biofilms and in a model food system", volume = "12", number = "10", pages = "1930", doi = "10.3390/foods12101930" }
Vidaković Knežević, S., Knežević, S., Vranešević, J., Kravić, S. Ž., Lakićević, B., Kocić-Tanackov, S.,& Karabasil, N.. (2023). Effects of selected essential oils on Listeria monocytogenes in biofilms and in a model food system. in Foods MDPI., 12(10), 1930. https://doi.org/10.3390/foods12101930
Vidaković Knežević S, Knežević S, Vranešević J, Kravić SŽ, Lakićević B, Kocić-Tanackov S, Karabasil N. Effects of selected essential oils on Listeria monocytogenes in biofilms and in a model food system. in Foods. 2023;12(10):1930. doi:10.3390/foods12101930 .
Vidaković Knežević, Suzana, Knežević, Slobodan, Vranešević, Jelena, Kravić, Snežana Ž., Lakićević, Brankica, Kocić-Tanackov, Sunčica, Karabasil, Nedjeljko, "Effects of selected essential oils on Listeria monocytogenes in biofilms and in a model food system" in Foods, 12, no. 10 (2023):1930, https://doi.org/10.3390/foods12101930 . .