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dc.creatorSpremo-Potparević, Biljana
dc.creatorZivković, L
dc.creatorĐelić, Ninoslav
dc.creatorBajić, V
dc.date.accessioned2020-06-03T12:52:02Z
dc.date.available2020-06-03T12:52:02Z
dc.date.issued2004
dc.identifier.issn0531-5565
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/294
dc.description.abstractCytogenetic analysis of the X chromosome in phytohaemagglutinin stimulated peripheral blood lymphocytes was evaluated in 12 sporadic Alzheimer disease (AD) patients and in 11 healthy subjects. For chromosome analysis two methods were used: (1) standard analysis of G-banded metaphase chromosomes and; (2) fluorescent in situ hybridization (FISH) for the detection of the X chromosome centromeric region in interphase nuclei. Cytogenetic analysis revealed that the X chromosome expresses premature centromere division (PCD) in AD females in 10.53% of metaphase cells and in 15.22% of interphase nuclei. In AD men the percentages were 3.98 and 6.06%, respectively. X chromosome PCD in the female control group showed a percentage of 7.46% in metaphase cells and 9.35% in interphase nuclei and in male controls the percentages were 2.84% in metaphases and 5.54% in interphase nuclei. The results of FISH analysis showed that PCD could occur much earlier than metaphase of mitosis, i.e. in interphase of the cell cycle, immediately after replication. The FISH method can be used for PCD verification in all phases of the cell cycle in various disorders including AD.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.rightsrestrictedAccess
dc.sourceExperimental Gerontology
dc.subjectAlzheimer diseaseen
dc.subjectfluorescent in situ hybridizationen
dc.subjectpremature centromere divisionen
dc.subjectX chromosomeen
dc.subjectcell cycleen
dc.titleAnalysis of premature centromere division (PCD) of the X chromosome in Alzheimer patients through the cell cycleen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЗивковић, Л; Бајић, В; Ђелић, Нинослав; Спремо-Потпаревић, Биљана;
dc.citation.volume39
dc.citation.issue5
dc.citation.spage849
dc.citation.epage854
dc.citation.other39(5): 849-854
dc.citation.rankM21
dc.identifier.wos000221633400019
dc.identifier.doi10.1016/j.exger.2004.01.012
dc.identifier.pmid15130680
dc.identifier.scopus2-s2.0-2342648875
dc.identifier.rcubconv_1453
dc.type.versionpublishedVersion


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