Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract
Аутори
Glavinić, UrošDžogović, Danica
Jelisić, Stefan
Ristanić, Marko
Zorc, Minja
Aleksić, Nevenka
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Nosema ceranae, a microsporidium species, is among the most common causes of bee diseases. The positive effect of Agaricus bisporus mushroom extract on the survival and immunity of Nosema-infected bees has been reported recently. The effect could be achieved by stimulating the expression of immune-related genes, but also by suppressing nosemosis. The aim of this work was to determine the effect of A. bisporus extract on the oxidative status of bees infected with N. ceranae. In a cage experiment on newly hatched bees, the effect of aqueous extract of champignon (A. bisporus, strain A15) was investigated. Six groups were formed: three groups were infected and received A. bisporus extract through food at different times (days 1, 3, and 6 after hatching), one group received the extract but was not infected (treatment control), one was only infected with Nosema (positive control) and one was neither infected nor received the extract (negative control). The effects were examined on samples t...aken on days 7 and 15 of the study. The activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) and the concentrations of malondialdehyde (MDA) were determined. In comparison to the positive control, the enzyme activities and MDA concentrations were significantly lower in the groups fed with the mushroom extract supplement. In the negative control, the level of oxidative stress was lower than in the positive control. In comparison with the other groups, the values mostly did not differ significantly. The oxidative status of bees infected with N. ceranae was significantly better if they were fed with the A. bisporus extract.
Кључне речи:
Apis mellifera / Nosema ceranae / Agaricus bisporus / oxidative stress / oxidative statusИзвор:
Veterinarski Glasnik, 2023, 77, 1, 35-50Издавач:
- University of Belgrade, Faculty of Veterinary Medicine
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Glavinić, Uroš AU - Džogović, Danica AU - Jelisić, Stefan AU - Ristanić, Marko AU - Zorc, Minja AU - Aleksić, Nevenka AU - Stanimirović, Zoran PY - 2023 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2953 AB - Nosema ceranae, a microsporidium species, is among the most common causes of bee diseases. The positive effect of Agaricus bisporus mushroom extract on the survival and immunity of Nosema-infected bees has been reported recently. The effect could be achieved by stimulating the expression of immune-related genes, but also by suppressing nosemosis. The aim of this work was to determine the effect of A. bisporus extract on the oxidative status of bees infected with N. ceranae. In a cage experiment on newly hatched bees, the effect of aqueous extract of champignon (A. bisporus, strain A15) was investigated. Six groups were formed: three groups were infected and received A. bisporus extract through food at different times (days 1, 3, and 6 after hatching), one group received the extract but was not infected (treatment control), one was only infected with Nosema (positive control) and one was neither infected nor received the extract (negative control). The effects were examined on samples taken on days 7 and 15 of the study. The activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) and the concentrations of malondialdehyde (MDA) were determined. In comparison to the positive control, the enzyme activities and MDA concentrations were significantly lower in the groups fed with the mushroom extract supplement. In the negative control, the level of oxidative stress was lower than in the positive control. In comparison with the other groups, the values mostly did not differ significantly. The oxidative status of bees infected with N. ceranae was significantly better if they were fed with the A. bisporus extract. PB - University of Belgrade, Faculty of Veterinary Medicine T2 - Veterinarski Glasnik T1 - Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract VL - 77 IS - 1 SP - 35 EP - 50 DO - 10.2298/VETGL220715013G ER -
@article{ author = "Glavinić, Uroš and Džogović, Danica and Jelisić, Stefan and Ristanić, Marko and Zorc, Minja and Aleksić, Nevenka and Stanimirović, Zoran", year = "2023", abstract = "Nosema ceranae, a microsporidium species, is among the most common causes of bee diseases. The positive effect of Agaricus bisporus mushroom extract on the survival and immunity of Nosema-infected bees has been reported recently. The effect could be achieved by stimulating the expression of immune-related genes, but also by suppressing nosemosis. The aim of this work was to determine the effect of A. bisporus extract on the oxidative status of bees infected with N. ceranae. In a cage experiment on newly hatched bees, the effect of aqueous extract of champignon (A. bisporus, strain A15) was investigated. Six groups were formed: three groups were infected and received A. bisporus extract through food at different times (days 1, 3, and 6 after hatching), one group received the extract but was not infected (treatment control), one was only infected with Nosema (positive control) and one was neither infected nor received the extract (negative control). The effects were examined on samples taken on days 7 and 15 of the study. The activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) and the concentrations of malondialdehyde (MDA) were determined. In comparison to the positive control, the enzyme activities and MDA concentrations were significantly lower in the groups fed with the mushroom extract supplement. In the negative control, the level of oxidative stress was lower than in the positive control. In comparison with the other groups, the values mostly did not differ significantly. The oxidative status of bees infected with N. ceranae was significantly better if they were fed with the A. bisporus extract.", publisher = "University of Belgrade, Faculty of Veterinary Medicine", journal = "Veterinarski Glasnik", title = "Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract", volume = "77", number = "1", pages = "35-50", doi = "10.2298/VETGL220715013G" }
Glavinić, U., Džogović, D., Jelisić, S., Ristanić, M., Zorc, M., Aleksić, N.,& Stanimirović, Z.. (2023). Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract. in Veterinarski Glasnik University of Belgrade, Faculty of Veterinary Medicine., 77(1), 35-50. https://doi.org/10.2298/VETGL220715013G
Glavinić U, Džogović D, Jelisić S, Ristanić M, Zorc M, Aleksić N, Stanimirović Z. Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract. in Veterinarski Glasnik. 2023;77(1):35-50. doi:10.2298/VETGL220715013G .
Glavinić, Uroš, Džogović, Danica, Jelisić, Stefan, Ristanić, Marko, Zorc, Minja, Aleksić, Nevenka, Stanimirović, Zoran, "Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract" in Veterinarski Glasnik, 77, no. 1 (2023):35-50, https://doi.org/10.2298/VETGL220715013G . .