Vitamin B1 kao potencijalni „antidot“ u lečenju Japanskih prepelica trovanih hlorpirifosom
Vitamin B1 as a potential "antidote" in the treatment of Japanese quail poisoned with chlorpyriphos
Аутори
Ćupić Miladinović, DejanaBorozan, Sunčica
Ivanović, Saša
Prevendar Crnić, Andreja
Đorđević, Milena
Blagojević, Miloš
Nešić, Ivana
Cukić, Nikola
Ćupić, Vitomir
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Hlorpirifos (CPF) je hlorovani organofosfatni pesticid, odnosno insekticid, koji se već dugo sa uspehom koristio u poljoprivredi. Ovaj insekticid svoje delovanje kod sisara ostvaruje na najmanje tri načina: inhibicijom aktivnosti enzima acetilholinesteraze (AChE), izazivanjem oksidativnog stresa i izazivanjem funkcionalnog poremećaja endokrinih žlezda. Za vitamin B1 (tiamin) je poznato da spada u grupu hidrosolubilnih vitamina, kao i da poseduje antioksidativni efekat. Osim toga, tiamin preko acetil koenzima A (acetil-CoA) učestvuje u sintezi acetilholinesteraze pa njegov nedostatak potencira delovanje organofostata.
Cilj naših ispitivanja bio je da se na japanskim prepelicama, trovanih hlorpirifosom, ispita da li i u kojoj meri vitamin B1 utiče na parametre oksidativnog stresa, inflamacije (interleukina 1(IL-1) i interleukina
6 (IL-6), apoptoze (inducibilne azot-oksidaze (iNOS) i ciklooksigenaze-2 (COX-2)), kao i aktivnost enzima acetil i butirilholinesteraze (AChE i BChE). Rezulta...ti ovih ispitivanja pokazali su da tiamin efikasno dovodi do oporavka aktivnosti AChE i BuChE u plazmi, jetri i mozgu, koja je značajno bila inhibisana
dejstvom CPF-a. Osim toga, tiamin pokazuje i antioksidativna svojstva, pošto povećava ukupnu koncentraciju redukovanog glutationa u mozgu, zatim smanjuje stepen lipidne peroksidacije, redukuje produkciju IL-1 i IL-6, snižava ekspresiju iNOS i COX-2. Dobijeni rezultati su pokazali da vitamin B1 ima
povoljno dejstvo kod prepelica, trovanih hlorpirifosom.
Chlorpyrifos (CPF) is a chlorinated organophosphate pesticide, i.e. insecticide, which was successfully used in agriculture for a long time. This insecticide exerts its action in mammals in at least three ways: by inhibiting the activity of the enzyme acetylcholinesterase (AChE), by causing oxidative stress, and
by causing functional disruption of the endocrine glands. Vitamin B1 (thiamine) is known to belong to the group of water-soluble vitamins, and has an antioxidant effect. In addition, thiamine through acetyl coenzyme A (acetyl-CoA) participates in the synthesis of acetylcholinesterase, so its deficiency
potentiates the action of organophosphates.
The aim of our work was to investigate whether and to what extent vitamin B1 affects the parameters of oxidative stress, inflammation (interleukin 1 (IL-1) and interleukin 6 (IL-6)), apoptosis (inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX-2)), as well as the activity of the enzymes acetyl and butyrylcholinesterase (AchE an...d BChE) and cyclooxygenase (COX), in Japanese quail poisoned with chlorpyrifos.
The results of these tests showed that thiamine effectively led to the recovery of AchE and BuChE activity in plasma, liver and brain, which was significantly inhibited by the action of CPF. In addition, thiamine also shows antioxidant properties as it increases the total concentration of reduced glutathione in the brain, then decreases the degree of lipid peroxidation, reduces the production of IL-1 and IL-6, and lowers the expression of iNOS and COX-2. The obtained results showed that vitamin B1 has a beneficial effect on quail poisoned by chlorpyrifos.
Кључне речи:
vitamin B1, Japanese quail, organophosphate pesticides, chlorpyrifos, oxidative stress parameters / vitamin B1, japanske prepelice, organofosfatni pesticidi, hlorpirifos, parametri oksidativnog stresaИзвор:
13th International Congress of the Serbian Society of Toxicology and 1st TOXSEE Regional Conference, belgrade, 10 - 12 May, 2023, 2023, 111-112Издавач:
- Belgrade : Serbian Society of Toxicology
Финансирање / пројекти:
- Унапређење и развој хигијенских и технолошких поступака у производњи намирница животињског порекла у циљу добијања квалитетних и безбедних производа конкурентних на светском тржишту (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
Напомена:
- Abstract book
Колекције
Институција/група
Fakultet veterinarske medicineTY - CONF AU - Ćupić Miladinović, Dejana AU - Borozan, Sunčica AU - Ivanović, Saša AU - Prevendar Crnić, Andreja AU - Đorđević, Milena AU - Blagojević, Miloš AU - Nešić, Ivana AU - Cukić, Nikola AU - Ćupić, Vitomir PY - 2023 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2963 AB - Hlorpirifos (CPF) je hlorovani organofosfatni pesticid, odnosno insekticid, koji se već dugo sa uspehom koristio u poljoprivredi. Ovaj insekticid svoje delovanje kod sisara ostvaruje na najmanje tri načina: inhibicijom aktivnosti enzima acetilholinesteraze (AChE), izazivanjem oksidativnog stresa i izazivanjem funkcionalnog poremećaja endokrinih žlezda. Za vitamin B1 (tiamin) je poznato da spada u grupu hidrosolubilnih vitamina, kao i da poseduje antioksidativni efekat. Osim toga, tiamin preko acetil koenzima A (acetil-CoA) učestvuje u sintezi acetilholinesteraze pa njegov nedostatak potencira delovanje organofostata. Cilj naših ispitivanja bio je da se na japanskim prepelicama, trovanih hlorpirifosom, ispita da li i u kojoj meri vitamin B1 utiče na parametre oksidativnog stresa, inflamacije (interleukina 1(IL-1) i interleukina 6 (IL-6), apoptoze (inducibilne azot-oksidaze (iNOS) i ciklooksigenaze-2 (COX-2)), kao i aktivnost enzima acetil i butirilholinesteraze (AChE i BChE). Rezultati ovih ispitivanja pokazali su da tiamin efikasno dovodi do oporavka aktivnosti AChE i BuChE u plazmi, jetri i mozgu, koja je značajno bila inhibisana dejstvom CPF-a. Osim toga, tiamin pokazuje i antioksidativna svojstva, pošto povećava ukupnu koncentraciju redukovanog glutationa u mozgu, zatim smanjuje stepen lipidne peroksidacije, redukuje produkciju IL-1 i IL-6, snižava ekspresiju iNOS i COX-2. Dobijeni rezultati su pokazali da vitamin B1 ima povoljno dejstvo kod prepelica, trovanih hlorpirifosom. AB - Chlorpyrifos (CPF) is a chlorinated organophosphate pesticide, i.e. insecticide, which was successfully used in agriculture for a long time. This insecticide exerts its action in mammals in at least three ways: by inhibiting the activity of the enzyme acetylcholinesterase (AChE), by causing oxidative stress, and by causing functional disruption of the endocrine glands. Vitamin B1 (thiamine) is known to belong to the group of water-soluble vitamins, and has an antioxidant effect. In addition, thiamine through acetyl coenzyme A (acetyl-CoA) participates in the synthesis of acetylcholinesterase, so its deficiency potentiates the action of organophosphates. The aim of our work was to investigate whether and to what extent vitamin B1 affects the parameters of oxidative stress, inflammation (interleukin 1 (IL-1) and interleukin 6 (IL-6)), apoptosis (inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX-2)), as well as the activity of the enzymes acetyl and butyrylcholinesterase (AchE and BChE) and cyclooxygenase (COX), in Japanese quail poisoned with chlorpyrifos. The results of these tests showed that thiamine effectively led to the recovery of AchE and BuChE activity in plasma, liver and brain, which was significantly inhibited by the action of CPF. In addition, thiamine also shows antioxidant properties as it increases the total concentration of reduced glutathione in the brain, then decreases the degree of lipid peroxidation, reduces the production of IL-1 and IL-6, and lowers the expression of iNOS and COX-2. The obtained results showed that vitamin B1 has a beneficial effect on quail poisoned by chlorpyrifos. PB - Belgrade : Serbian Society of Toxicology C3 - 13th International Congress of the Serbian Society of Toxicology and 1st TOXSEE Regional Conference, belgrade, 10 - 12 May, 2023 T1 - Vitamin B1 kao potencijalni „antidot“ u lečenju Japanskih prepelica trovanih hlorpirifosom T1 - Vitamin B1 as a potential "antidote" in the treatment of Japanese quail poisoned with chlorpyriphos SP - 111 EP - 112 UR - https://hdl.handle.net/21.15107/rcub_veterinar_2963 ER -
@conference{ author = "Ćupić Miladinović, Dejana and Borozan, Sunčica and Ivanović, Saša and Prevendar Crnić, Andreja and Đorđević, Milena and Blagojević, Miloš and Nešić, Ivana and Cukić, Nikola and Ćupić, Vitomir", year = "2023", abstract = "Hlorpirifos (CPF) je hlorovani organofosfatni pesticid, odnosno insekticid, koji se već dugo sa uspehom koristio u poljoprivredi. Ovaj insekticid svoje delovanje kod sisara ostvaruje na najmanje tri načina: inhibicijom aktivnosti enzima acetilholinesteraze (AChE), izazivanjem oksidativnog stresa i izazivanjem funkcionalnog poremećaja endokrinih žlezda. Za vitamin B1 (tiamin) je poznato da spada u grupu hidrosolubilnih vitamina, kao i da poseduje antioksidativni efekat. Osim toga, tiamin preko acetil koenzima A (acetil-CoA) učestvuje u sintezi acetilholinesteraze pa njegov nedostatak potencira delovanje organofostata. Cilj naših ispitivanja bio je da se na japanskim prepelicama, trovanih hlorpirifosom, ispita da li i u kojoj meri vitamin B1 utiče na parametre oksidativnog stresa, inflamacije (interleukina 1(IL-1) i interleukina 6 (IL-6), apoptoze (inducibilne azot-oksidaze (iNOS) i ciklooksigenaze-2 (COX-2)), kao i aktivnost enzima acetil i butirilholinesteraze (AChE i BChE). Rezultati ovih ispitivanja pokazali su da tiamin efikasno dovodi do oporavka aktivnosti AChE i BuChE u plazmi, jetri i mozgu, koja je značajno bila inhibisana dejstvom CPF-a. Osim toga, tiamin pokazuje i antioksidativna svojstva, pošto povećava ukupnu koncentraciju redukovanog glutationa u mozgu, zatim smanjuje stepen lipidne peroksidacije, redukuje produkciju IL-1 i IL-6, snižava ekspresiju iNOS i COX-2. Dobijeni rezultati su pokazali da vitamin B1 ima povoljno dejstvo kod prepelica, trovanih hlorpirifosom., Chlorpyrifos (CPF) is a chlorinated organophosphate pesticide, i.e. insecticide, which was successfully used in agriculture for a long time. This insecticide exerts its action in mammals in at least three ways: by inhibiting the activity of the enzyme acetylcholinesterase (AChE), by causing oxidative stress, and by causing functional disruption of the endocrine glands. Vitamin B1 (thiamine) is known to belong to the group of water-soluble vitamins, and has an antioxidant effect. In addition, thiamine through acetyl coenzyme A (acetyl-CoA) participates in the synthesis of acetylcholinesterase, so its deficiency potentiates the action of organophosphates. The aim of our work was to investigate whether and to what extent vitamin B1 affects the parameters of oxidative stress, inflammation (interleukin 1 (IL-1) and interleukin 6 (IL-6)), apoptosis (inducible nitric oxide (iNOS) and cyclooxygenase-2 (COX-2)), as well as the activity of the enzymes acetyl and butyrylcholinesterase (AchE and BChE) and cyclooxygenase (COX), in Japanese quail poisoned with chlorpyrifos. The results of these tests showed that thiamine effectively led to the recovery of AchE and BuChE activity in plasma, liver and brain, which was significantly inhibited by the action of CPF. In addition, thiamine also shows antioxidant properties as it increases the total concentration of reduced glutathione in the brain, then decreases the degree of lipid peroxidation, reduces the production of IL-1 and IL-6, and lowers the expression of iNOS and COX-2. The obtained results showed that vitamin B1 has a beneficial effect on quail poisoned by chlorpyrifos.", publisher = "Belgrade : Serbian Society of Toxicology", journal = "13th International Congress of the Serbian Society of Toxicology and 1st TOXSEE Regional Conference, belgrade, 10 - 12 May, 2023", title = "Vitamin B1 kao potencijalni „antidot“ u lečenju Japanskih prepelica trovanih hlorpirifosom, Vitamin B1 as a potential "antidote" in the treatment of Japanese quail poisoned with chlorpyriphos", pages = "111-112", url = "https://hdl.handle.net/21.15107/rcub_veterinar_2963" }
Ćupić Miladinović, D., Borozan, S., Ivanović, S., Prevendar Crnić, A., Đorđević, M., Blagojević, M., Nešić, I., Cukić, N.,& Ćupić, V.. (2023). Vitamin B1 kao potencijalni „antidot“ u lečenju Japanskih prepelica trovanih hlorpirifosom. in 13th International Congress of the Serbian Society of Toxicology and 1st TOXSEE Regional Conference, belgrade, 10 - 12 May, 2023 Belgrade : Serbian Society of Toxicology., 111-112. https://hdl.handle.net/21.15107/rcub_veterinar_2963
Ćupić Miladinović D, Borozan S, Ivanović S, Prevendar Crnić A, Đorđević M, Blagojević M, Nešić I, Cukić N, Ćupić V. Vitamin B1 kao potencijalni „antidot“ u lečenju Japanskih prepelica trovanih hlorpirifosom. in 13th International Congress of the Serbian Society of Toxicology and 1st TOXSEE Regional Conference, belgrade, 10 - 12 May, 2023. 2023;:111-112. https://hdl.handle.net/21.15107/rcub_veterinar_2963 .
Ćupić Miladinović, Dejana, Borozan, Sunčica, Ivanović, Saša, Prevendar Crnić, Andreja, Đorđević, Milena, Blagojević, Miloš, Nešić, Ivana, Cukić, Nikola, Ćupić, Vitomir, "Vitamin B1 kao potencijalni „antidot“ u lečenju Japanskih prepelica trovanih hlorpirifosom" in 13th International Congress of the Serbian Society of Toxicology and 1st TOXSEE Regional Conference, belgrade, 10 - 12 May, 2023 (2023):111-112, https://hdl.handle.net/21.15107/rcub_veterinar_2963 .