Genotoxicity potential of 8-Cl-cyclic adenosine monophosphate assessed with cytogenetic tests in vivo
Abstract
Background. Growth-modulating noncytotoxic activity of 8-chloro-adenosine 3,5-cyclic monophosphate (8-Cl-cAMP) showed inhibitory effect on growth of a wide variety of cancer cell lines in vitro and in vivo. To assess possible genotoxic effects of 8-Cl-cAMP, we conducted a study in vivo using male BALB/c mice. Methods. Clastogenic effects were estimated by bone marrow micronucleus assay and cytogenetic test in adult mice BALB/c strain. 8-Cl-cAMP was administered intraperitoneally (i.p.) to three dose groups including 10 mg/kg body weight (b.w.), 90 mg/kg b.w., and 160 mg/kg b.w., with saline solution as negative control and cyclophosphamide, a known mutagen, and clastogen as positive control during a 7-day period in 24-h intervals. Results. Micronucleus test in vivo results showed consistently increasing dose-dependent pattern increase of dose regime (10 mg/kg body weight [b.w.], 90 mg/kg b.w., and 160 mg/kg b.w.), and increase in frequency of micronuclei in polychromatic erythrocytes (...4.88 +/- 0.35, 8.32 +/- 0.57, and 11.74 +/- 0.37) compared to negative control (2.04 +/- 0.28). Quantitative effects are paralleled by structural changes in chromosome morphology. 8-Cl-cAMP induced structural (breaks, gaps, centric rings, acentrics, and Robertsonian translocations) and numerical-type chromosomal aberrations (aneuploidy and polyploidy). Conclusions. Results of this study demonstrate that 8-Cl-cAMP has genotoxic potential in vivo.
Keywords:
antitumor agent 8-Cl-cAMP / genotoxicity / micronucleus test / numerical and structural chromosomal aberrationsSource:
Archives of Medical Research, 2004, 35, 3, 209-214Publisher:
- Elsevier Science Inc, New York
DOI: 10.1016/j.arcmed.2004.01.004
ISSN: 0188-4409
PubMed: 15163461
WoS: 000221906400004
Scopus: 2-s2.0-2442421673
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Bajić, Vladan AU - Stanimirović, Zoran AU - Stevanović, Jevrosima PY - 2004 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/306 AB - Background. Growth-modulating noncytotoxic activity of 8-chloro-adenosine 3,5-cyclic monophosphate (8-Cl-cAMP) showed inhibitory effect on growth of a wide variety of cancer cell lines in vitro and in vivo. To assess possible genotoxic effects of 8-Cl-cAMP, we conducted a study in vivo using male BALB/c mice. Methods. Clastogenic effects were estimated by bone marrow micronucleus assay and cytogenetic test in adult mice BALB/c strain. 8-Cl-cAMP was administered intraperitoneally (i.p.) to three dose groups including 10 mg/kg body weight (b.w.), 90 mg/kg b.w., and 160 mg/kg b.w., with saline solution as negative control and cyclophosphamide, a known mutagen, and clastogen as positive control during a 7-day period in 24-h intervals. Results. Micronucleus test in vivo results showed consistently increasing dose-dependent pattern increase of dose regime (10 mg/kg body weight [b.w.], 90 mg/kg b.w., and 160 mg/kg b.w.), and increase in frequency of micronuclei in polychromatic erythrocytes (4.88 +/- 0.35, 8.32 +/- 0.57, and 11.74 +/- 0.37) compared to negative control (2.04 +/- 0.28). Quantitative effects are paralleled by structural changes in chromosome morphology. 8-Cl-cAMP induced structural (breaks, gaps, centric rings, acentrics, and Robertsonian translocations) and numerical-type chromosomal aberrations (aneuploidy and polyploidy). Conclusions. Results of this study demonstrate that 8-Cl-cAMP has genotoxic potential in vivo. PB - Elsevier Science Inc, New York T2 - Archives of Medical Research T1 - Genotoxicity potential of 8-Cl-cyclic adenosine monophosphate assessed with cytogenetic tests in vivo VL - 35 IS - 3 SP - 209 EP - 214 DO - 10.1016/j.arcmed.2004.01.004 ER -
@article{ author = "Bajić, Vladan and Stanimirović, Zoran and Stevanović, Jevrosima", year = "2004", abstract = "Background. Growth-modulating noncytotoxic activity of 8-chloro-adenosine 3,5-cyclic monophosphate (8-Cl-cAMP) showed inhibitory effect on growth of a wide variety of cancer cell lines in vitro and in vivo. To assess possible genotoxic effects of 8-Cl-cAMP, we conducted a study in vivo using male BALB/c mice. Methods. Clastogenic effects were estimated by bone marrow micronucleus assay and cytogenetic test in adult mice BALB/c strain. 8-Cl-cAMP was administered intraperitoneally (i.p.) to three dose groups including 10 mg/kg body weight (b.w.), 90 mg/kg b.w., and 160 mg/kg b.w., with saline solution as negative control and cyclophosphamide, a known mutagen, and clastogen as positive control during a 7-day period in 24-h intervals. Results. Micronucleus test in vivo results showed consistently increasing dose-dependent pattern increase of dose regime (10 mg/kg body weight [b.w.], 90 mg/kg b.w., and 160 mg/kg b.w.), and increase in frequency of micronuclei in polychromatic erythrocytes (4.88 +/- 0.35, 8.32 +/- 0.57, and 11.74 +/- 0.37) compared to negative control (2.04 +/- 0.28). Quantitative effects are paralleled by structural changes in chromosome morphology. 8-Cl-cAMP induced structural (breaks, gaps, centric rings, acentrics, and Robertsonian translocations) and numerical-type chromosomal aberrations (aneuploidy and polyploidy). Conclusions. Results of this study demonstrate that 8-Cl-cAMP has genotoxic potential in vivo.", publisher = "Elsevier Science Inc, New York", journal = "Archives of Medical Research", title = "Genotoxicity potential of 8-Cl-cyclic adenosine monophosphate assessed with cytogenetic tests in vivo", volume = "35", number = "3", pages = "209-214", doi = "10.1016/j.arcmed.2004.01.004" }
Bajić, V., Stanimirović, Z.,& Stevanović, J.. (2004). Genotoxicity potential of 8-Cl-cyclic adenosine monophosphate assessed with cytogenetic tests in vivo. in Archives of Medical Research Elsevier Science Inc, New York., 35(3), 209-214. https://doi.org/10.1016/j.arcmed.2004.01.004
Bajić V, Stanimirović Z, Stevanović J. Genotoxicity potential of 8-Cl-cyclic adenosine monophosphate assessed with cytogenetic tests in vivo. in Archives of Medical Research. 2004;35(3):209-214. doi:10.1016/j.arcmed.2004.01.004 .
Bajić, Vladan, Stanimirović, Zoran, Stevanović, Jevrosima, "Genotoxicity potential of 8-Cl-cyclic adenosine monophosphate assessed with cytogenetic tests in vivo" in Archives of Medical Research, 35, no. 3 (2004):209-214, https://doi.org/10.1016/j.arcmed.2004.01.004 . .