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dc.creatorBajić, V
dc.creatorStanimirović, Zoran
dc.creatorStevanović, Jevrosima
dc.date.accessioned2020-06-03T12:52:52Z
dc.date.available2020-06-03T12:52:52Z
dc.date.issued2004
dc.identifier.issn0188-4409
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/306
dc.description.abstractBackground. Growth-modulating noncytotoxic activity of 8-chloro-adenosine 3,5-cyclic monophosphate (8-Cl-cAMP) showed inhibitory effect on growth of a wide variety of cancer cell lines in vitro and in vivo. To assess possible genotoxic effects of 8-Cl-cAMP, we conducted a study in vivo using male BALB/c mice. Methods. Clastogenic effects were estimated by bone marrow micronucleus assay and cytogenetic test in adult mice BALB/c strain. 8-Cl-cAMP was administered intraperitoneally (i.p.) to three dose groups including 10 mg/kg body weight (b.w.), 90 mg/kg b.w., and 160 mg/kg b.w., with saline solution as negative control and cyclophosphamide, a known mutagen, and clastogen as positive control during a 7-day period in 24-h intervals. Results. Micronucleus test in vivo results showed consistently increasing dose-dependent pattern increase of dose regime (10 mg/kg body weight [b.w.], 90 mg/kg b.w., and 160 mg/kg b.w.), and increase in frequency of micronuclei in polychromatic erythrocytes (4.88 +/- 0.35, 8.32 +/- 0.57, and 11.74 +/- 0.37) compared to negative control (2.04 +/- 0.28). Quantitative effects are paralleled by structural changes in chromosome morphology. 8-Cl-cAMP induced structural (breaks, gaps, centric rings, acentrics, and Robertsonian translocations) and numerical-type chromosomal aberrations (aneuploidy and polyploidy). Conclusions. Results of this study demonstrate that 8-Cl-cAMP has genotoxic potential in vivo.en
dc.publisherElsevier Science Inc, New York
dc.rightsrestrictedAccess
dc.sourceArchives of Medical Research
dc.subjectantitumor agent 8-Cl-cAMPen
dc.subjectgenotoxicityen
dc.subjectmicronucleus testen
dc.subjectnumerical and structural chromosomal aberrationsen
dc.titleGenotoxicity potential of 8-Cl-cyclic adenosine monophosphate assessed with cytogenetic tests in vivoen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractБајић, В; Станимировић, Зоран; Стевановић, Јевросима;
dc.citation.volume35
dc.citation.issue3
dc.citation.spage209
dc.citation.epage214
dc.citation.other35(3): 209-214
dc.citation.rankM23
dc.identifier.wos000221906400004
dc.identifier.doi10.1016/j.arcmed.2004.01.004
dc.identifier.pmid15163461
dc.identifier.scopus2-s2.0-2442421673
dc.identifier.rcubconv_1454
dc.type.versionpublishedVersion


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