Porcine stress syndrome (PSS) and ryanodine receptor 1 (RYR1) gene mutation in European wild pig (Sus scrota ferus)

Abstract

Expression of the stress syndrome (PSS) in European wild pig was evaluated by Halothane test and tested animals were typed by PCR/RFLP test for C/T mutation at nt 1843 in RYR1 gene. A total of 56 animals, at the age of 5 months, weighing 20 kg average, were tested. Hal+ (MHS - malignant hyperthermia susceptible) phenotype was revealed in 6 pigs, 47 animals expressed Hal- (MHN - malignant hypedhermia resistant) phenotype, while 3 animals showed no response to administration of 3% halothane after 5 minutes and they were marked as Hair (halothane resistant) phenotypes. Genotyping of 56 wild pigs revealed that the tested population was homozygous for the normal C/C allele at RYR1 locus. The results obtained by halothane test show that expression of the PSS in wild pigs is modulated and influenced by factors other then RYR1 genotype.

Pojava stres sindroma kod evropske divlje svinje ispitivana je primenom halotan testa i izvršena je genotipizacija na poziciji nukleotida 1843 u genu za rijanodin receptor (RYR1) korišćenjem PCR/restriktivnog endonukleaza testa. Testirano je ukupno 56 životinja, starosti 5 meseci i prosečne telesne mase od 20 kg. Od ukupno 56 jedinki 6 životinja je pokazalo Hal+ (MHS) fenotip, 47 jedinki je imalo Hal+ (MHN) fenotip, a tri jedinke nisu uopšte reagovale na 3% halotan ni posle 5 minuta uvođenja u narkozu i one su označene kao Hair fenotip. Genotipizacija na poziciji nukleotida 1843 u genu za rijanodin receptor pokazala je da su sve ispitivane jedinke, bez obzira na halotan status imale normalan RYR1 C/C genotip. Dobijeni rezultati ukazuju da se stres sindrom javlja i kod divljih svinja, mada postoji značajna modulacija pune ekspresije sindroma drugim faktorima.