Eight-Year Study of Haemogregarina stepanowi Infection in Poached European Pond Turtles (Emys orbicularis) Held in Belgrade Zoo Quarantine
Аутори
Aleksić-Kovačević, SanjaVučićević, Miloš
Özvegy, József
Jelisić, Stefan
Djurdjević, Biljana
Prodanov-Radulović, Jasna
Došenović, Milan
Marinković, Darko
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The eight-year study (2015–2023) was performed on a large sample of poached European
pond turtles infected with Haemogregarina stepanowi and held in a pond that belongs to a quarantine
section of Belgrade Zoo. The protected species of European pond turtles have been found in poor
health, with general weakness, anorexia, and low motility. Comprehensive cytological, hematological,
molecular, and postmortem evaluations have been performed. Initially, Diff Quick staining of the
blood smears revealed rounded or elongated erythrocytes, often bearing premeront or U-shaped
gamont of the hemogregarines inside. The reduced erythrocyte numbers, hemoglobin, and hematocrit
values found in the examined population of infected turtles indicated anemia. Macroscopically, shell
necrosis and massive skin hemorrhages were the most prominent findings observed in diseased
turtles. Microscopically, the lungs, liver, kidneys, and spleen revealed hyperemia, hemorrhages,
and the presence of parasitic s...tages in tissue samples in 31 of 40 necropsied turtles. Cytological
and microscopic examination of the samples proved to be sufficient for establishing the infection,
but molecular analyses of the 18S sequence were used for phylogenetic studies. Over the years, the
number of diseased and dead turtles has decreased, which could be hypothetically attributed to the
elimination of leeches as the definitive host.
Кључне речи:
European pond turtle / Haemogregarina stepanowi / Serbia / zoo quarantineИзвор:
Animals, 2023, 13, 15, 2429-Издавач:
- MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Aleksić-Kovačević, Sanja AU - Vučićević, Miloš AU - Özvegy, József AU - Jelisić, Stefan AU - Djurdjević, Biljana AU - Prodanov-Radulović, Jasna AU - Došenović, Milan AU - Marinković, Darko PY - 2023 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3156 AB - The eight-year study (2015–2023) was performed on a large sample of poached European pond turtles infected with Haemogregarina stepanowi and held in a pond that belongs to a quarantine section of Belgrade Zoo. The protected species of European pond turtles have been found in poor health, with general weakness, anorexia, and low motility. Comprehensive cytological, hematological, molecular, and postmortem evaluations have been performed. Initially, Diff Quick staining of the blood smears revealed rounded or elongated erythrocytes, often bearing premeront or U-shaped gamont of the hemogregarines inside. The reduced erythrocyte numbers, hemoglobin, and hematocrit values found in the examined population of infected turtles indicated anemia. Macroscopically, shell necrosis and massive skin hemorrhages were the most prominent findings observed in diseased turtles. Microscopically, the lungs, liver, kidneys, and spleen revealed hyperemia, hemorrhages, and the presence of parasitic stages in tissue samples in 31 of 40 necropsied turtles. Cytological and microscopic examination of the samples proved to be sufficient for establishing the infection, but molecular analyses of the 18S sequence were used for phylogenetic studies. Over the years, the number of diseased and dead turtles has decreased, which could be hypothetically attributed to the elimination of leeches as the definitive host. PB - MDPI T2 - Animals T1 - Eight-Year Study of Haemogregarina stepanowi Infection in Poached European Pond Turtles (Emys orbicularis) Held in Belgrade Zoo Quarantine VL - 13 IS - 15 SP - 2429 DO - 10.3390/ani13152429 ER -
@article{ author = "Aleksić-Kovačević, Sanja and Vučićević, Miloš and Özvegy, József and Jelisić, Stefan and Djurdjević, Biljana and Prodanov-Radulović, Jasna and Došenović, Milan and Marinković, Darko", year = "2023", abstract = "The eight-year study (2015–2023) was performed on a large sample of poached European pond turtles infected with Haemogregarina stepanowi and held in a pond that belongs to a quarantine section of Belgrade Zoo. The protected species of European pond turtles have been found in poor health, with general weakness, anorexia, and low motility. Comprehensive cytological, hematological, molecular, and postmortem evaluations have been performed. Initially, Diff Quick staining of the blood smears revealed rounded or elongated erythrocytes, often bearing premeront or U-shaped gamont of the hemogregarines inside. The reduced erythrocyte numbers, hemoglobin, and hematocrit values found in the examined population of infected turtles indicated anemia. Macroscopically, shell necrosis and massive skin hemorrhages were the most prominent findings observed in diseased turtles. Microscopically, the lungs, liver, kidneys, and spleen revealed hyperemia, hemorrhages, and the presence of parasitic stages in tissue samples in 31 of 40 necropsied turtles. Cytological and microscopic examination of the samples proved to be sufficient for establishing the infection, but molecular analyses of the 18S sequence were used for phylogenetic studies. Over the years, the number of diseased and dead turtles has decreased, which could be hypothetically attributed to the elimination of leeches as the definitive host.", publisher = "MDPI", journal = "Animals", title = "Eight-Year Study of Haemogregarina stepanowi Infection in Poached European Pond Turtles (Emys orbicularis) Held in Belgrade Zoo Quarantine", volume = "13", number = "15", pages = "2429", doi = "10.3390/ani13152429" }
Aleksić-Kovačević, S., Vučićević, M., Özvegy, J., Jelisić, S., Djurdjević, B., Prodanov-Radulović, J., Došenović, M.,& Marinković, D.. (2023). Eight-Year Study of Haemogregarina stepanowi Infection in Poached European Pond Turtles (Emys orbicularis) Held in Belgrade Zoo Quarantine. in Animals MDPI., 13(15), 2429. https://doi.org/10.3390/ani13152429
Aleksić-Kovačević S, Vučićević M, Özvegy J, Jelisić S, Djurdjević B, Prodanov-Radulović J, Došenović M, Marinković D. Eight-Year Study of Haemogregarina stepanowi Infection in Poached European Pond Turtles (Emys orbicularis) Held in Belgrade Zoo Quarantine. in Animals. 2023;13(15):2429. doi:10.3390/ani13152429 .
Aleksić-Kovačević, Sanja, Vučićević, Miloš, Özvegy, József, Jelisić, Stefan, Djurdjević, Biljana, Prodanov-Radulović, Jasna, Došenović, Milan, Marinković, Darko, "Eight-Year Study of Haemogregarina stepanowi Infection in Poached European Pond Turtles (Emys orbicularis) Held in Belgrade Zoo Quarantine" in Animals, 13, no. 15 (2023):2429, https://doi.org/10.3390/ani13152429 . .