Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in Holstein Friesian dairy cows with subclinical endometritis
Аутори
Milovanović, BojanKureljušić, Branislav
Milićević, Vesna
Zdravković, Nemanja
Kureljušić, Jasna
Marinković, Darko
Maletić, Milan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Mycoplasma spp. is the cause of serious cattle health disorders that lead to poor
reproductive efficiency. Chronic seminal vesiculitis, infertility, vulvovaginitis and
dystocia were observed in Mycoplasma spp. infection. The objectives of the present study
were to investigate the frequency of Mycoplasmas in the uterus of postpartum dairy
cows and its potential role in the occurrence of subclinical endometritis. Our study
included 102 Holstein Friesian dairy cows 22-32 days postpartum. Uterine samples for
cytological, bacteriological, and molecular analysis were provided using Cytoprint AI®.
Metricheck® was used for the assessment of cervicovaginal mucus. After detection
of Mycoplasma spp. genome in uterine samples, the swabs of the vulva, vagina, nasal
swab, and samples of milk were sampled in order to exclude possible contamination
with other microorganisms as potential trigger of subclinical endometritis. The genome
of Mycoplasma spp. in the uterus was confirmed in 4 cows ...(2.40%). Sequencing of
the 16S RNA revealed that detected mycoplasmas belonged to Mycoplasma bovigenitalium
and Mycoplasma tauri species, with a prevalence of 1.80% and 0.6%, respectively. The
cytological evaluation showed a 69.90%, 54.87% and 48.33% of polymorphonuclear cells
for M. bovigenitalium positive cows, and 61.64% for M. tauri positive cow. Bacteriological
examination revealed Trueperella pyogenes in uterine samples in all 4 observed cows. This
is the first study where M. tauri was detected in the uterus of a live cow with subclinical enodmetritis. In this paper we hypothesized that M. bovigenitalium and M. tauri may have
a certain role in the etiology of subclinical enodmetritis.
Кључне речи:
dairy cows / Mycoplasma bovigenitalium / Mycoplasma spp / Mycoplasma tauri / subclinical endometritisИзвор:
Acta Veterinaria, 2023, 73, 3, 421-431Издавач:
- Beograd : Fakultet veterinarske medicine
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Milovanović, Bojan AU - Kureljušić, Branislav AU - Milićević, Vesna AU - Zdravković, Nemanja AU - Kureljušić, Jasna AU - Marinković, Darko AU - Maletić, Milan PY - 2023 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3264 AB - Mycoplasma spp. is the cause of serious cattle health disorders that lead to poor reproductive efficiency. Chronic seminal vesiculitis, infertility, vulvovaginitis and dystocia were observed in Mycoplasma spp. infection. The objectives of the present study were to investigate the frequency of Mycoplasmas in the uterus of postpartum dairy cows and its potential role in the occurrence of subclinical endometritis. Our study included 102 Holstein Friesian dairy cows 22-32 days postpartum. Uterine samples for cytological, bacteriological, and molecular analysis were provided using Cytoprint AI®. Metricheck® was used for the assessment of cervicovaginal mucus. After detection of Mycoplasma spp. genome in uterine samples, the swabs of the vulva, vagina, nasal swab, and samples of milk were sampled in order to exclude possible contamination with other microorganisms as potential trigger of subclinical endometritis. The genome of Mycoplasma spp. in the uterus was confirmed in 4 cows (2.40%). Sequencing of the 16S RNA revealed that detected mycoplasmas belonged to Mycoplasma bovigenitalium and Mycoplasma tauri species, with a prevalence of 1.80% and 0.6%, respectively. The cytological evaluation showed a 69.90%, 54.87% and 48.33% of polymorphonuclear cells for M. bovigenitalium positive cows, and 61.64% for M. tauri positive cow. Bacteriological examination revealed Trueperella pyogenes in uterine samples in all 4 observed cows. This is the first study where M. tauri was detected in the uterus of a live cow with subclinical enodmetritis. In this paper we hypothesized that M. bovigenitalium and M. tauri may have a certain role in the etiology of subclinical enodmetritis. PB - Beograd : Fakultet veterinarske medicine T2 - Acta Veterinaria T1 - Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in Holstein Friesian dairy cows with subclinical endometritis VL - 73 IS - 3 SP - 421 EP - 431 DO - 10.2478/acve-2023-0031 ER -
@article{ author = "Milovanović, Bojan and Kureljušić, Branislav and Milićević, Vesna and Zdravković, Nemanja and Kureljušić, Jasna and Marinković, Darko and Maletić, Milan", year = "2023", abstract = "Mycoplasma spp. is the cause of serious cattle health disorders that lead to poor reproductive efficiency. Chronic seminal vesiculitis, infertility, vulvovaginitis and dystocia were observed in Mycoplasma spp. infection. The objectives of the present study were to investigate the frequency of Mycoplasmas in the uterus of postpartum dairy cows and its potential role in the occurrence of subclinical endometritis. Our study included 102 Holstein Friesian dairy cows 22-32 days postpartum. Uterine samples for cytological, bacteriological, and molecular analysis were provided using Cytoprint AI®. Metricheck® was used for the assessment of cervicovaginal mucus. After detection of Mycoplasma spp. genome in uterine samples, the swabs of the vulva, vagina, nasal swab, and samples of milk were sampled in order to exclude possible contamination with other microorganisms as potential trigger of subclinical endometritis. The genome of Mycoplasma spp. in the uterus was confirmed in 4 cows (2.40%). Sequencing of the 16S RNA revealed that detected mycoplasmas belonged to Mycoplasma bovigenitalium and Mycoplasma tauri species, with a prevalence of 1.80% and 0.6%, respectively. The cytological evaluation showed a 69.90%, 54.87% and 48.33% of polymorphonuclear cells for M. bovigenitalium positive cows, and 61.64% for M. tauri positive cow. Bacteriological examination revealed Trueperella pyogenes in uterine samples in all 4 observed cows. This is the first study where M. tauri was detected in the uterus of a live cow with subclinical enodmetritis. In this paper we hypothesized that M. bovigenitalium and M. tauri may have a certain role in the etiology of subclinical enodmetritis.", publisher = "Beograd : Fakultet veterinarske medicine", journal = "Acta Veterinaria", title = "Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in Holstein Friesian dairy cows with subclinical endometritis", volume = "73", number = "3", pages = "421-431", doi = "10.2478/acve-2023-0031" }
Milovanović, B., Kureljušić, B., Milićević, V., Zdravković, N., Kureljušić, J., Marinković, D.,& Maletić, M.. (2023). Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in Holstein Friesian dairy cows with subclinical endometritis. in Acta Veterinaria Beograd : Fakultet veterinarske medicine., 73(3), 421-431. https://doi.org/10.2478/acve-2023-0031
Milovanović B, Kureljušić B, Milićević V, Zdravković N, Kureljušić J, Marinković D, Maletić M. Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in Holstein Friesian dairy cows with subclinical endometritis. in Acta Veterinaria. 2023;73(3):421-431. doi:10.2478/acve-2023-0031 .
Milovanović, Bojan, Kureljušić, Branislav, Milićević, Vesna, Zdravković, Nemanja, Kureljušić, Jasna, Marinković, Darko, Maletić, Milan, "Detection of Mycoplasma bovigenitalium and Mycoplasma tauri in Holstein Friesian dairy cows with subclinical endometritis" in Acta Veterinaria, 73, no. 3 (2023):421-431, https://doi.org/10.2478/acve-2023-0031 . .
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