Effects of plant-based supplement on oxidative stress of honey bees (Apis mellifera) infected with Nosema ceranae
Аутори
Jovanović, Nemanja M.Glavinić, Uroš
Ristanić, Marko
Vejnović, Branislav
Ilić, Tamara
Stevanović, Jevrosima
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
One of the most important approaches in the prevention and treatment of nosemosis is the use of herbal preparations as food supplements for bees. Therefore, the aim of this study was to investigate the effects of a plant-based supplement branded as “B+” on honeybees in a laboratory experiment. Four experimental groups were established: treated group (T), N. ceranae-infected and treated group (IT), N. ceranae-infected group (I) and non-infected group (NI). Survival, N. ceranae spore load and oxidative stress parameters together with expression levels of antioxidant enzyme genes and vitellogenin gene were monitored. The mortality in the T, IT and NI groups was significantly (p < 0.001) lower than in than in the I group. Within Nosema-infected groups, the IT group had a significantly lower (p < 0.001) number of N. ceranae spores than the I group. In addition, expression levels of genes for antioxidant enzymes were lower (p < 0.001) in the IT group compared to the I group. The concentratio...n of malondialdehyde and the activities of antioxidant enzymes (superoxide dismutase, catalase and glutathione S-transferase) were significantly lower (p < 0.001) in the IT group compared to the I group. No negative effects of the tested supplement were observed. All these findings indicate that the tested supplement exerted beneficial effects manifested in better bee survival, reduced N. ceranae spore number and reduced oxidative stress of bees (lower expression of genes for antioxidant enzymes and oxidative stress parameters).
Кључне речи:
dietary supplement / Nosema ceranae / oxidative stress / gene expression / vitellogeninИзвор:
Animals, 2023, 13, 22, 3543-Издавач:
- MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Jovanović, Nemanja M. AU - Glavinić, Uroš AU - Ristanić, Marko AU - Vejnović, Branislav AU - Ilić, Tamara AU - Stevanović, Jevrosima AU - Stanimirović, Zoran PY - 2023 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3370 AB - One of the most important approaches in the prevention and treatment of nosemosis is the use of herbal preparations as food supplements for bees. Therefore, the aim of this study was to investigate the effects of a plant-based supplement branded as “B+” on honeybees in a laboratory experiment. Four experimental groups were established: treated group (T), N. ceranae-infected and treated group (IT), N. ceranae-infected group (I) and non-infected group (NI). Survival, N. ceranae spore load and oxidative stress parameters together with expression levels of antioxidant enzyme genes and vitellogenin gene were monitored. The mortality in the T, IT and NI groups was significantly (p < 0.001) lower than in than in the I group. Within Nosema-infected groups, the IT group had a significantly lower (p < 0.001) number of N. ceranae spores than the I group. In addition, expression levels of genes for antioxidant enzymes were lower (p < 0.001) in the IT group compared to the I group. The concentration of malondialdehyde and the activities of antioxidant enzymes (superoxide dismutase, catalase and glutathione S-transferase) were significantly lower (p < 0.001) in the IT group compared to the I group. No negative effects of the tested supplement were observed. All these findings indicate that the tested supplement exerted beneficial effects manifested in better bee survival, reduced N. ceranae spore number and reduced oxidative stress of bees (lower expression of genes for antioxidant enzymes and oxidative stress parameters). PB - MDPI T2 - Animals T1 - Effects of plant-based supplement on oxidative stress of honey bees (Apis mellifera) infected with Nosema ceranae VL - 13 IS - 22 SP - 3543 DO - 10.3390/ani13223543 ER -
@article{ author = "Jovanović, Nemanja M. and Glavinić, Uroš and Ristanić, Marko and Vejnović, Branislav and Ilić, Tamara and Stevanović, Jevrosima and Stanimirović, Zoran", year = "2023", abstract = "One of the most important approaches in the prevention and treatment of nosemosis is the use of herbal preparations as food supplements for bees. Therefore, the aim of this study was to investigate the effects of a plant-based supplement branded as “B+” on honeybees in a laboratory experiment. Four experimental groups were established: treated group (T), N. ceranae-infected and treated group (IT), N. ceranae-infected group (I) and non-infected group (NI). Survival, N. ceranae spore load and oxidative stress parameters together with expression levels of antioxidant enzyme genes and vitellogenin gene were monitored. The mortality in the T, IT and NI groups was significantly (p < 0.001) lower than in than in the I group. Within Nosema-infected groups, the IT group had a significantly lower (p < 0.001) number of N. ceranae spores than the I group. In addition, expression levels of genes for antioxidant enzymes were lower (p < 0.001) in the IT group compared to the I group. The concentration of malondialdehyde and the activities of antioxidant enzymes (superoxide dismutase, catalase and glutathione S-transferase) were significantly lower (p < 0.001) in the IT group compared to the I group. No negative effects of the tested supplement were observed. All these findings indicate that the tested supplement exerted beneficial effects manifested in better bee survival, reduced N. ceranae spore number and reduced oxidative stress of bees (lower expression of genes for antioxidant enzymes and oxidative stress parameters).", publisher = "MDPI", journal = "Animals", title = "Effects of plant-based supplement on oxidative stress of honey bees (Apis mellifera) infected with Nosema ceranae", volume = "13", number = "22", pages = "3543", doi = "10.3390/ani13223543" }
Jovanović, N. M., Glavinić, U., Ristanić, M., Vejnović, B., Ilić, T., Stevanović, J.,& Stanimirović, Z.. (2023). Effects of plant-based supplement on oxidative stress of honey bees (Apis mellifera) infected with Nosema ceranae. in Animals MDPI., 13(22), 3543. https://doi.org/10.3390/ani13223543
Jovanović NM, Glavinić U, Ristanić M, Vejnović B, Ilić T, Stevanović J, Stanimirović Z. Effects of plant-based supplement on oxidative stress of honey bees (Apis mellifera) infected with Nosema ceranae. in Animals. 2023;13(22):3543. doi:10.3390/ani13223543 .
Jovanović, Nemanja M., Glavinić, Uroš, Ristanić, Marko, Vejnović, Branislav, Ilić, Tamara, Stevanović, Jevrosima, Stanimirović, Zoran, "Effects of plant-based supplement on oxidative stress of honey bees (Apis mellifera) infected with Nosema ceranae" in Animals, 13, no. 22 (2023):3543, https://doi.org/10.3390/ani13223543 . .