Evaluation of genotoxic effects of Apitol (R) (cymiazole hydrochloride) in vitro by measurement of sister chromatid exchange

Abstract

Apitol((R)), with cymiazole hydrochloride as the active ingredient. is used in bee-keeping, against the ectoparasitic mite Varroa destructor. The preparation was evaluated for genotoxicity in cultured human peripheral blood lymphocytes, Sister chromatid exchange, the mitotic index and the cell proliferation index were determined for three experimental concentrations of Apitol((R)), (0.001, 0.01 and 0.1 mg/ml). All concentrations significantly (p < 0.001) increased the mitotic index (MI = 7.35 +/- 0,18% 831 +/- 0.20% and 12.33 +/- 0.25%, respectively), the proliferative index (PI = 1.83 +/- 0.01, 1.84 +/- 0.01 and 1.88 +/- 0.02. respectively) and the frequency of sister chromatid exchange (SCE = 8.19 +/- 1.81, 8.78 +/- 1.80 and 13 46 +/- 1.88, respectively), suggesting that cymiazole hydrochloride has genotoxic potential.