Genomic insights into methicillin-resistant staphylococci and mammaliicocci from bulk tank milk of dairy farms in Serbia
Аутори
Kos, AndreaPapić, Bojan
Golob, Majda
Avberšek, Jana
Kušar, Darja
Ledina, Tijana
Đorđević, Jasna
Bulajić, Snežana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The potential risk to human and animal health provides a rationale for research on
methicillin-resistant staphylococci (MRS) and mammaliicocci (MRM) in dairy herds. Here, we
aimed to estimate their occurrence in the bulk tank milk (BTM) samples collected in 2019–2021 from
283 bovine dairy farms in the Belgrade district. We used whole-genome sequencing to characterize
the obtained isolates and assess their genetic relatedness. A total of 70 MRS/MRM were recovered,
most frequently Staphylococcus haemolyticus and Mammaliicoccus sciuri. Five clusters of 2–4 genetically
related isolates were identified and epidemiological data indicated transmission through, e.g., farm
visits by personnel or milk collection trucks. Most MRSA isolates belonged to the typical livestockassociated
lineage ST398-t034. One MRSA isolate (ST152-t355) harbored the PVL-encoding genes.
Since MRS/MRM isolates obtained in this study frequently harbored genes conferring multidrug
resistance (MDR), this argues f...or their role as reservoirs for the spread of antimicrobial resistance
genes. The pipeline milking system and total bacterial count >100,000 CFU/mL were significantly
associated with higher occurrences of MRS/MRM. Our study confirms that BTM can be a zoonotic
source of MRS, including MDR strains. This highlights the urgent need for good agricultural practices
and the continuous monitoring of MRS/MRM in dairy farms.
Кључне речи:
methicillin-resistant staphylococci/mammaliicocci / methicillin-resistant Staphylococcus aureus / bulk tank milk / antimicrobial resistance / virulence factors / whole-genome sequencingИзвор:
Antibiotics, 2023, 12, 10, 1529-Издавач:
- MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
- Directorate for National Reference Laboratories, Belgrade, Serbia and by the Slovenian Research and Innovation Agency (research core funding no. P4-0092 ‘Animal health, environment and food safety’ and Slovenia-Serbia bilateral project grant no. BI-RS/23-25-007 ‘Raw milk as a potential source of multidrug resistant bacteria and resistance genes’)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Kos, Andrea AU - Papić, Bojan AU - Golob, Majda AU - Avberšek, Jana AU - Kušar, Darja AU - Ledina, Tijana AU - Đorđević, Jasna AU - Bulajić, Snežana PY - 2023 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3429 AB - The potential risk to human and animal health provides a rationale for research on methicillin-resistant staphylococci (MRS) and mammaliicocci (MRM) in dairy herds. Here, we aimed to estimate their occurrence in the bulk tank milk (BTM) samples collected in 2019–2021 from 283 bovine dairy farms in the Belgrade district. We used whole-genome sequencing to characterize the obtained isolates and assess their genetic relatedness. A total of 70 MRS/MRM were recovered, most frequently Staphylococcus haemolyticus and Mammaliicoccus sciuri. Five clusters of 2–4 genetically related isolates were identified and epidemiological data indicated transmission through, e.g., farm visits by personnel or milk collection trucks. Most MRSA isolates belonged to the typical livestockassociated lineage ST398-t034. One MRSA isolate (ST152-t355) harbored the PVL-encoding genes. Since MRS/MRM isolates obtained in this study frequently harbored genes conferring multidrug resistance (MDR), this argues for their role as reservoirs for the spread of antimicrobial resistance genes. The pipeline milking system and total bacterial count >100,000 CFU/mL were significantly associated with higher occurrences of MRS/MRM. Our study confirms that BTM can be a zoonotic source of MRS, including MDR strains. This highlights the urgent need for good agricultural practices and the continuous monitoring of MRS/MRM in dairy farms. PB - MDPI T2 - Antibiotics T1 - Genomic insights into methicillin-resistant staphylococci and mammaliicocci from bulk tank milk of dairy farms in Serbia VL - 12 IS - 10 SP - 1529 DO - 10.3390/antibiotics12101529 ER -
@article{ author = "Kos, Andrea and Papić, Bojan and Golob, Majda and Avberšek, Jana and Kušar, Darja and Ledina, Tijana and Đorđević, Jasna and Bulajić, Snežana", year = "2023", abstract = "The potential risk to human and animal health provides a rationale for research on methicillin-resistant staphylococci (MRS) and mammaliicocci (MRM) in dairy herds. Here, we aimed to estimate their occurrence in the bulk tank milk (BTM) samples collected in 2019–2021 from 283 bovine dairy farms in the Belgrade district. We used whole-genome sequencing to characterize the obtained isolates and assess their genetic relatedness. A total of 70 MRS/MRM were recovered, most frequently Staphylococcus haemolyticus and Mammaliicoccus sciuri. Five clusters of 2–4 genetically related isolates were identified and epidemiological data indicated transmission through, e.g., farm visits by personnel or milk collection trucks. Most MRSA isolates belonged to the typical livestockassociated lineage ST398-t034. One MRSA isolate (ST152-t355) harbored the PVL-encoding genes. Since MRS/MRM isolates obtained in this study frequently harbored genes conferring multidrug resistance (MDR), this argues for their role as reservoirs for the spread of antimicrobial resistance genes. The pipeline milking system and total bacterial count >100,000 CFU/mL were significantly associated with higher occurrences of MRS/MRM. Our study confirms that BTM can be a zoonotic source of MRS, including MDR strains. This highlights the urgent need for good agricultural practices and the continuous monitoring of MRS/MRM in dairy farms.", publisher = "MDPI", journal = "Antibiotics", title = "Genomic insights into methicillin-resistant staphylococci and mammaliicocci from bulk tank milk of dairy farms in Serbia", volume = "12", number = "10", pages = "1529", doi = "10.3390/antibiotics12101529" }
Kos, A., Papić, B., Golob, M., Avberšek, J., Kušar, D., Ledina, T., Đorđević, J.,& Bulajić, S.. (2023). Genomic insights into methicillin-resistant staphylococci and mammaliicocci from bulk tank milk of dairy farms in Serbia. in Antibiotics MDPI., 12(10), 1529. https://doi.org/10.3390/antibiotics12101529
Kos A, Papić B, Golob M, Avberšek J, Kušar D, Ledina T, Đorđević J, Bulajić S. Genomic insights into methicillin-resistant staphylococci and mammaliicocci from bulk tank milk of dairy farms in Serbia. in Antibiotics. 2023;12(10):1529. doi:10.3390/antibiotics12101529 .
Kos, Andrea, Papić, Bojan, Golob, Majda, Avberšek, Jana, Kušar, Darja, Ledina, Tijana, Đorđević, Jasna, Bulajić, Snežana, "Genomic insights into methicillin-resistant staphylococci and mammaliicocci from bulk tank milk of dairy farms in Serbia" in Antibiotics, 12, no. 10 (2023):1529, https://doi.org/10.3390/antibiotics12101529 . .