Napredak u veterinarskoj regenerativnoj medicini: mogućnost korišćenja biomaterijala na bazi kalcijum fosfata na eksperimentalnom modelu pacova
The improvement in veterinary regenerative medicine: the posibillity of using the biomaterials based on calciumphosphates on rat experimental model
Аутори
Marković, DanicaKojić, Zvezdana
Kovačević Filipović, Milica
Radovanović, Anita
Andrić, Nenad
Francuski, Jelena
Todorović, Vera
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Regenerative medicine is the field of human and veterinary medicine that involves creating living, functional tissue to repair
and replace tissues or organs that have been damaged by injury, disease, aging or birth defects. Regenerative adult cell
therapy with or without supporting biocompatible material, has been currently successfully treated in horses and dogs,
especially in joint, bone and ligament injuries.
The aim of this study was to evaluate the biocompatibility of three different bioceramical materials (BCM) implanted
(paravertebral lumbal region) in rat subcutaneous tissue according to the International standard procedures (ISO 10993-6;
ISO 10993-10) for screening tests by histological examinations. In order for biocompatibility phenomena to be investigated
and to choose of an implantable device we use the routine histological techniques (H&E, TB, AZAN),
immunohistochemical method (for labeling T and B lymphocytes and macrophages) and scanning electron microscopic
in...vestigations on interface of biomaterials. Thirty two rats were assigned to 4 groups, as following: control group; dental
ceramic (DC) implants; alfa tricalcium phosphate (áTCP); hydroxyapatite (HAP). The irritation index (the level of tissue
injuring) was noted microscopically and tabelar system of evaluations were compared in grading scores from 0 to 4, where
results had been described from mild (1) to severe (4). The inflammatory infiltrate consisted of lymphocytes,
polimorphonuclears, macrophages, siderophages and mastocytes. At the same time there were sign of encapsulation and
neovasculogenesis around BCM.
In conclusion, the irritation index among the different BCM were in all cases in the range of biocompatibility. Evaluating
different responses according to BCM, the most intense tissue reaction was noticed at HAP, grading as severe and mild to
moderate for DC and áTCP.
Кључне речи:
Regenerative medicine / bioceramics / biocompatibility / histologyИзвор:
16. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosan i Hercegovina) sa međunarodnim učešćem, Teslić, 1 - 4. jun 2011, 2011, 70-71Издавач:
- Banja Luka : Veterinarska komora Republike Srpske
Напомена:
- Zbornik kratkih sadržaja
Колекције
Институција/група
Fakultet veterinarske medicineTY - CONF AU - Marković, Danica AU - Kojić, Zvezdana AU - Kovačević Filipović, Milica AU - Radovanović, Anita AU - Andrić, Nenad AU - Francuski, Jelena AU - Todorović, Vera PY - 2011 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3605 AB - Regenerative medicine is the field of human and veterinary medicine that involves creating living, functional tissue to repair and replace tissues or organs that have been damaged by injury, disease, aging or birth defects. Regenerative adult cell therapy with or without supporting biocompatible material, has been currently successfully treated in horses and dogs, especially in joint, bone and ligament injuries. The aim of this study was to evaluate the biocompatibility of three different bioceramical materials (BCM) implanted (paravertebral lumbal region) in rat subcutaneous tissue according to the International standard procedures (ISO 10993-6; ISO 10993-10) for screening tests by histological examinations. In order for biocompatibility phenomena to be investigated and to choose of an implantable device we use the routine histological techniques (H&E, TB, AZAN), immunohistochemical method (for labeling T and B lymphocytes and macrophages) and scanning electron microscopic investigations on interface of biomaterials. Thirty two rats were assigned to 4 groups, as following: control group; dental ceramic (DC) implants; alfa tricalcium phosphate (áTCP); hydroxyapatite (HAP). The irritation index (the level of tissue injuring) was noted microscopically and tabelar system of evaluations were compared in grading scores from 0 to 4, where results had been described from mild (1) to severe (4). The inflammatory infiltrate consisted of lymphocytes, polimorphonuclears, macrophages, siderophages and mastocytes. At the same time there were sign of encapsulation and neovasculogenesis around BCM. In conclusion, the irritation index among the different BCM were in all cases in the range of biocompatibility. Evaluating different responses according to BCM, the most intense tissue reaction was noticed at HAP, grading as severe and mild to moderate for DC and áTCP. PB - Banja Luka : Veterinarska komora Republike Srpske C3 - 16. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosan i Hercegovina) sa međunarodnim učešćem, Teslić, 1 - 4. jun 2011 T1 - Napredak u veterinarskoj regenerativnoj medicini: mogućnost korišćenja biomaterijala na bazi kalcijum fosfata na eksperimentalnom modelu pacova T1 - The improvement in veterinary regenerative medicine: the posibillity of using the biomaterials based on calciumphosphates on rat experimental model SP - 70 EP - 71 UR - https://hdl.handle.net/21.15107/rcub_veterinar_3605 ER -
@conference{ author = "Marković, Danica and Kojić, Zvezdana and Kovačević Filipović, Milica and Radovanović, Anita and Andrić, Nenad and Francuski, Jelena and Todorović, Vera", year = "2011", abstract = "Regenerative medicine is the field of human and veterinary medicine that involves creating living, functional tissue to repair and replace tissues or organs that have been damaged by injury, disease, aging or birth defects. Regenerative adult cell therapy with or without supporting biocompatible material, has been currently successfully treated in horses and dogs, especially in joint, bone and ligament injuries. The aim of this study was to evaluate the biocompatibility of three different bioceramical materials (BCM) implanted (paravertebral lumbal region) in rat subcutaneous tissue according to the International standard procedures (ISO 10993-6; ISO 10993-10) for screening tests by histological examinations. In order for biocompatibility phenomena to be investigated and to choose of an implantable device we use the routine histological techniques (H&E, TB, AZAN), immunohistochemical method (for labeling T and B lymphocytes and macrophages) and scanning electron microscopic investigations on interface of biomaterials. Thirty two rats were assigned to 4 groups, as following: control group; dental ceramic (DC) implants; alfa tricalcium phosphate (áTCP); hydroxyapatite (HAP). The irritation index (the level of tissue injuring) was noted microscopically and tabelar system of evaluations were compared in grading scores from 0 to 4, where results had been described from mild (1) to severe (4). The inflammatory infiltrate consisted of lymphocytes, polimorphonuclears, macrophages, siderophages and mastocytes. At the same time there were sign of encapsulation and neovasculogenesis around BCM. In conclusion, the irritation index among the different BCM were in all cases in the range of biocompatibility. Evaluating different responses according to BCM, the most intense tissue reaction was noticed at HAP, grading as severe and mild to moderate for DC and áTCP.", publisher = "Banja Luka : Veterinarska komora Republike Srpske", journal = "16. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosan i Hercegovina) sa međunarodnim učešćem, Teslić, 1 - 4. jun 2011", title = "Napredak u veterinarskoj regenerativnoj medicini: mogućnost korišćenja biomaterijala na bazi kalcijum fosfata na eksperimentalnom modelu pacova, The improvement in veterinary regenerative medicine: the posibillity of using the biomaterials based on calciumphosphates on rat experimental model", pages = "70-71", url = "https://hdl.handle.net/21.15107/rcub_veterinar_3605" }
Marković, D., Kojić, Z., Kovačević Filipović, M., Radovanović, A., Andrić, N., Francuski, J.,& Todorović, V.. (2011). Napredak u veterinarskoj regenerativnoj medicini: mogućnost korišćenja biomaterijala na bazi kalcijum fosfata na eksperimentalnom modelu pacova. in 16. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosan i Hercegovina) sa međunarodnim učešćem, Teslić, 1 - 4. jun 2011 Banja Luka : Veterinarska komora Republike Srpske., 70-71. https://hdl.handle.net/21.15107/rcub_veterinar_3605
Marković D, Kojić Z, Kovačević Filipović M, Radovanović A, Andrić N, Francuski J, Todorović V. Napredak u veterinarskoj regenerativnoj medicini: mogućnost korišćenja biomaterijala na bazi kalcijum fosfata na eksperimentalnom modelu pacova. in 16. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosan i Hercegovina) sa međunarodnim učešćem, Teslić, 1 - 4. jun 2011. 2011;:70-71. https://hdl.handle.net/21.15107/rcub_veterinar_3605 .
Marković, Danica, Kojić, Zvezdana, Kovačević Filipović, Milica, Radovanović, Anita, Andrić, Nenad, Francuski, Jelena, Todorović, Vera, "Napredak u veterinarskoj regenerativnoj medicini: mogućnost korišćenja biomaterijala na bazi kalcijum fosfata na eksperimentalnom modelu pacova" in 16. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosan i Hercegovina) sa međunarodnim učešćem, Teslić, 1 - 4. jun 2011 (2011):70-71, https://hdl.handle.net/21.15107/rcub_veterinar_3605 .