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Servis period i antitela protiv spermatozoida kod veštački osemenjavanih krava

dc.creatorMilovanović, Aleksandar
dc.creatorLazarević, Miodrag
dc.creatorMilanović, Svetlana
dc.creatorKirovski, Danijela
dc.creatorJovičin, Milovan
dc.date.accessioned2020-06-03T12:56:44Z
dc.date.available2020-06-03T12:56:44Z
dc.date.issued2005
dc.identifier.issn0567-8315
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/361
dc.description.abstractThe aim of this study was to determine titers of antisperm antibodies (ASA) of Ig G and Ig A class in sera and cervical mucus of artificially inseminated Holstein cows in order to correlate these results with the duration of the open days period. Investigations were conducted on a total of 181 cows originating from three different dairy farms. Blood and cervical mucus samples for laboratory analyses were collected on the day of the last artificial insemination. Presence of ASA was determined by indirect immunofluorescence method (IIF) using bulls' sperm cells prepared for artificial insemination by suspending in TRIS - egg yolk or "Biociphos + " extenders prior to deep freezing. Our results strongly confirm the hypothesis that immune mechanisms may be involved in reproductive disturbances due to high levels of ASA of lg A class. In the sera and cervical mucus of cows, high levels of ASA were found in animals with longer open days period. In this study we were not able to demonstrate differences in ASA titers when sperm cells were suspended in different extenders and used for the IIF test.en
dc.description.abstractU ovom radu su izneti rezultati ispitivanja titra antitela Ig G i Ig A klase poreklom iz krvnog seruma i cervikalne sluzi veštački osemenjavanih holštajn krava protiv antigena spermatozoida bika. Ispitivanja su izvedena na ukupnom broju od 181 plotkinje sa tri regionalne farme muznih krava. Uzorci krvi i cervikalne sluzi su prikupljeni na dan poslednjeg veštačkog osemenjavanja. Titar antitela protiv antigena spermatozoida (ASA) je određivan metodom indirektne imunofluorescence a za izvođenje testa su korišćeni spermatozoidi suspendovani u TRIS žumanjčanom ili "Biociphos +" razređivaču. Naši rezultati potvrđuju hipotezu da su imunski mehanizmi uključeni u nastanak nekih reproduktivnih poremećaja jer su plotkinje sa visokim titrom ASA u serumu i cervikalnoj sluzi imala duži servis period. Osim toga, u ovim ispitivanjima nismo utvrdili postojanje razlika u titru ASA u zavisnosti od vrste korišćenog razređivača.sr
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.rightsopenAccess
dc.sourceActa veterinaria - Beograd
dc.subjectsperm cellsen
dc.subjectcowsen
dc.subjectartificial inseminationen
dc.subjectsub fertilityen
dc.subjectantisperm antibodiesen
dc.titleOpen days period and antispermatozoal antibodies in artificially inseminated cowsen
dc.titleServis period i antitela protiv spermatozoida kod veštački osemenjavanih kravasr
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМилановић, Светлана; Лазаревић, Миодраг; Јовичин, Милован; Кировски, Данијела; Миловановић, Aлександар; Сервис период и антитела против сперматозоида код вештачки осемењаваних крава; Сервис период и антитела против сперматозоида код вештачки осемењаваних крава;
dc.citation.volume55
dc.citation.issue5-6
dc.citation.spage449
dc.citation.epage460
dc.citation.other55(5-6): 449-460
dc.citation.rankM23
dc.identifier.wos000234035600011
dc.identifier.doi10.2298/AVB0506449M
dc.identifier.scopus2-s2.0-29344454333
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/926/360.pdf
dc.identifier.rcubconv_629
dc.type.versionpublishedVersion


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