Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line
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2024
Authors
Bruić, MarijaPirković, Andrea
Borozan, Sunčica
Nacka Aleksić, Mirjana
Jovanović Krivokuća, Milica
Spremo-Potparević, Biljana
Article (Published version)
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Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant secondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 µM and 100 µM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant enzymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed antiinflammatory activi...ty in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and antiinflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing potential as a therapeutic agent in preventing adverse pregnancy outcomes.
Keywords:
Taxifolin / Trophoblast / Oxidative stress / ROS / InflammationSource:
Reproductive Toxicology, 2024, 126, 108585-Publisher:
- Elsevier
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200161 (University of Belgrade, Faculty of Pharmacy) (RS-MESTD-inst-2020-200161)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200019 (University of Belgrade, Institute for the Application of Nuclear Energy - INEP) (RS-MESTD-inst-2020-200019)
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Fakultet veterinarske medicineTY - JOUR AU - Bruić, Marija AU - Pirković, Andrea AU - Borozan, Sunčica AU - Nacka Aleksić, Mirjana AU - Jovanović Krivokuća, Milica AU - Spremo-Potparević, Biljana PY - 2024 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3864 AB - Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant secondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 µM and 100 µM concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content, extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant enzymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed antiinflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and antiinflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing potential as a therapeutic agent in preventing adverse pregnancy outcomes. PB - Elsevier T2 - Reproductive Toxicology T1 - Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line VL - 126 SP - 108585 DO - 10.1016/j.reprotox.2024.108585 ER -
@article{ author = "Bruić, Marija and Pirković, Andrea and Borozan, Sunčica and Nacka Aleksić, Mirjana and Jovanović Krivokuća, Milica and Spremo-Potparević, Biljana", year = "2024", abstract = "Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant secondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 µM and 100 µM concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content, extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant enzymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed antiinflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and antiinflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing potential as a therapeutic agent in preventing adverse pregnancy outcomes.", publisher = "Elsevier", journal = "Reproductive Toxicology", title = "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line", volume = "126", pages = "108585", doi = "10.1016/j.reprotox.2024.108585" }
Bruić, M., Pirković, A., Borozan, S., Nacka Aleksić, M., Jovanović Krivokuća, M.,& Spremo-Potparević, B.. (2024). Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology Elsevier., 126, 108585. https://doi.org/10.1016/j.reprotox.2024.108585
Bruić M, Pirković A, Borozan S, Nacka Aleksić M, Jovanović Krivokuća M, Spremo-Potparević B. Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology. 2024;126:108585. doi:10.1016/j.reprotox.2024.108585 .
Bruić, Marija, Pirković, Andrea, Borozan, Sunčica, Nacka Aleksić, Mirjana, Jovanović Krivokuća, Milica, Spremo-Potparević, Biljana, "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line" in Reproductive Toxicology, 126 (2024):108585, https://doi.org/10.1016/j.reprotox.2024.108585 . .