Presence of Mycoplasma bovis in bulk tank milk and associated risk factor analysis in Serbian dairy farms
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Mycoplasma bovis (M. bovis) is a significant pathogen responsible for highly transmissible
mastitis in cattle globally. It primarily spreads through colostrum, milk, and semen. Cows with
persistent infections act as carriers, intermittently releasing the pathogen, making their milk a pivotal
factor in infection transmission. Given the limited seroprevalence surveys in Serbia, this study aimed
to detect M. bovis presence in bulk tank milk (BTM), determine route shedding, and evaluate infection
risks. BTM samples were collected from 115 dairy farms across Serbia, with M. bovis DNA detected
in 11 out of the 115 samples by real-time PCR. Additionally, M. bovis was detected in 1.30% of nasal
swabs sampled from apparently healthy animals. A univariate analysis of the risk factors associated
with M. bovis presence in the BTM samples revealed correlations with factors such as the breed, farm
seropositivity, pre-milking and post-milking disinfection practices, farm type, cow population..., milk
yield, number of cows in the BTM samples, and parity. Seropositive farms exhibited the highest
likelihood of M. bovis presence in milk. Moreover, pre- and post-milking disinfection practices and
highly productive cows yielding over 8000 L of milk were identified as risk factors for PCR-positive
BTM. In a multivariable mixed regression analysis, a risk factor for the presence of M. bovis infection
in the BTM sample was the Holstein breed. These findings underscore a relatively high prevalence
of M. bovis in BTM within Serbian dairy farms, suggesting a potential risk for M. bovis spreading
through milk and oral route of calves’ infection.
Кључне речи:
Mycoplasma bovis / bulk tank milk / risk factors / SerbiaИзвор:
Pathogens, 2024, 13, 4, 302-Издавач:
- MDPI
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200030 (Научни институт за ветеринарство Србије, Београд) (RS-MESTD-inst-2020-200030)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200143 (Универзитет у Београду, Факултет ветеринарске медицине) (RS-MESTD-inst-2020-200143)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Ninković, Milan AU - Milićević, Vesna AU - Radojičić, Sonja AU - Bugarski, Dejan AU - Stević, Nataša PY - 2024 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3869 AB - Mycoplasma bovis (M. bovis) is a significant pathogen responsible for highly transmissible mastitis in cattle globally. It primarily spreads through colostrum, milk, and semen. Cows with persistent infections act as carriers, intermittently releasing the pathogen, making their milk a pivotal factor in infection transmission. Given the limited seroprevalence surveys in Serbia, this study aimed to detect M. bovis presence in bulk tank milk (BTM), determine route shedding, and evaluate infection risks. BTM samples were collected from 115 dairy farms across Serbia, with M. bovis DNA detected in 11 out of the 115 samples by real-time PCR. Additionally, M. bovis was detected in 1.30% of nasal swabs sampled from apparently healthy animals. A univariate analysis of the risk factors associated with M. bovis presence in the BTM samples revealed correlations with factors such as the breed, farm seropositivity, pre-milking and post-milking disinfection practices, farm type, cow population, milk yield, number of cows in the BTM samples, and parity. Seropositive farms exhibited the highest likelihood of M. bovis presence in milk. Moreover, pre- and post-milking disinfection practices and highly productive cows yielding over 8000 L of milk were identified as risk factors for PCR-positive BTM. In a multivariable mixed regression analysis, a risk factor for the presence of M. bovis infection in the BTM sample was the Holstein breed. These findings underscore a relatively high prevalence of M. bovis in BTM within Serbian dairy farms, suggesting a potential risk for M. bovis spreading through milk and oral route of calves’ infection. PB - MDPI T2 - Pathogens T1 - Presence of Mycoplasma bovis in bulk tank milk and associated risk factor analysis in Serbian dairy farms VL - 13 IS - 4 SP - 302 DO - 10.3390/pathogens13040302 ER -
@article{ author = "Ninković, Milan and Milićević, Vesna and Radojičić, Sonja and Bugarski, Dejan and Stević, Nataša", year = "2024", abstract = "Mycoplasma bovis (M. bovis) is a significant pathogen responsible for highly transmissible mastitis in cattle globally. It primarily spreads through colostrum, milk, and semen. Cows with persistent infections act as carriers, intermittently releasing the pathogen, making their milk a pivotal factor in infection transmission. Given the limited seroprevalence surveys in Serbia, this study aimed to detect M. bovis presence in bulk tank milk (BTM), determine route shedding, and evaluate infection risks. BTM samples were collected from 115 dairy farms across Serbia, with M. bovis DNA detected in 11 out of the 115 samples by real-time PCR. Additionally, M. bovis was detected in 1.30% of nasal swabs sampled from apparently healthy animals. A univariate analysis of the risk factors associated with M. bovis presence in the BTM samples revealed correlations with factors such as the breed, farm seropositivity, pre-milking and post-milking disinfection practices, farm type, cow population, milk yield, number of cows in the BTM samples, and parity. Seropositive farms exhibited the highest likelihood of M. bovis presence in milk. Moreover, pre- and post-milking disinfection practices and highly productive cows yielding over 8000 L of milk were identified as risk factors for PCR-positive BTM. In a multivariable mixed regression analysis, a risk factor for the presence of M. bovis infection in the BTM sample was the Holstein breed. These findings underscore a relatively high prevalence of M. bovis in BTM within Serbian dairy farms, suggesting a potential risk for M. bovis spreading through milk and oral route of calves’ infection.", publisher = "MDPI", journal = "Pathogens", title = "Presence of Mycoplasma bovis in bulk tank milk and associated risk factor analysis in Serbian dairy farms", volume = "13", number = "4", pages = "302", doi = "10.3390/pathogens13040302" }
Ninković, M., Milićević, V., Radojičić, S., Bugarski, D.,& Stević, N.. (2024). Presence of Mycoplasma bovis in bulk tank milk and associated risk factor analysis in Serbian dairy farms. in Pathogens MDPI., 13(4), 302. https://doi.org/10.3390/pathogens13040302
Ninković M, Milićević V, Radojičić S, Bugarski D, Stević N. Presence of Mycoplasma bovis in bulk tank milk and associated risk factor analysis in Serbian dairy farms. in Pathogens. 2024;13(4):302. doi:10.3390/pathogens13040302 .
Ninković, Milan, Milićević, Vesna, Radojičić, Sonja, Bugarski, Dejan, Stević, Nataša, "Presence of Mycoplasma bovis in bulk tank milk and associated risk factor analysis in Serbian dairy farms" in Pathogens, 13, no. 4 (2024):302, https://doi.org/10.3390/pathogens13040302 . .