Investigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle Disease virus envelope in vitro
Ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti in vitro
Abstract
The objective of these investigations was to examine the hemadsorption characteristics of glycoprotein HN and F antigens of the Newcastle Disease virus envelope. Six hours following inoculation of the cell line with the Newcastle Disease Vero virus, previously activated with 0.025 g/dl trypsin-versene, the appearance of hemadsorption was established in a smaller intensity in antigens diluted in ratios from 1:8 to 1:16. Hemadsorption was also determined in the mentioned cell lines 12 hours following inoculation in viruses diluted 1:64, while hemadsorption was present in virus dilutions of 1:256 after 24 hours. Inhibition of hemadsorption in inoculated cells with immune serum dilutions of 1:64 was established 24 hours following inoculation of the cell line with Vero samples of the Newcastle Disease virus, activated with trypsin-versene and the addition of specific immune serum.
Cilj naših istraživanja bilo je ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti. Posle 6 časova od inokulacije ćelijske linije Vero virusom Newcastle bolesti, prethodno aktivisanim sa 0,025 g/dl tripsinversena, pojava hemadsorpcije je ustanovljena u manjem intenzitetu u razređenjima antigena od 1:8 do 1:16. Hemadsorpcija je ustanovljena u pomenutim ćelijskim linijama i posle 12 časova od inokulacije u razređenju virusa od 1:64, dok je posle 24 časa od inokulacije hemadsorpcija bila prisutna i u razređenju virusa od 1:256. Posle 24 časa od inokulacije ćelijske linije Vero uzorcima virusa Newcastle bolesti, aktivisanih tripsin-versenom i dodavanja specifičnog imunog seruma, ustanovljena je inhibicija hemadsorpcije kod inokulisanih ćelija sa razređenjem imunog seruma od 1:64.
Keywords:
Newcastle Disease virus / Vero cells / hemadsorption / hemadsorption inhibition / Virus Newcastle bolesti / Vero ćelije / hemadsorpcija / inhibicija hemadsorpcijeSource:
Veterinarski Glasnik, 2006, 60, 5-6, 299-304Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
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Fakultet veterinarske medicineTY - JOUR AU - Nišavić, Jakov AU - Milić, Nenad PY - 2006 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/387 AB - The objective of these investigations was to examine the hemadsorption characteristics of glycoprotein HN and F antigens of the Newcastle Disease virus envelope. Six hours following inoculation of the cell line with the Newcastle Disease Vero virus, previously activated with 0.025 g/dl trypsin-versene, the appearance of hemadsorption was established in a smaller intensity in antigens diluted in ratios from 1:8 to 1:16. Hemadsorption was also determined in the mentioned cell lines 12 hours following inoculation in viruses diluted 1:64, while hemadsorption was present in virus dilutions of 1:256 after 24 hours. Inhibition of hemadsorption in inoculated cells with immune serum dilutions of 1:64 was established 24 hours following inoculation of the cell line with Vero samples of the Newcastle Disease virus, activated with trypsin-versene and the addition of specific immune serum. AB - Cilj naših istraživanja bilo je ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti. Posle 6 časova od inokulacije ćelijske linije Vero virusom Newcastle bolesti, prethodno aktivisanim sa 0,025 g/dl tripsinversena, pojava hemadsorpcije je ustanovljena u manjem intenzitetu u razređenjima antigena od 1:8 do 1:16. Hemadsorpcija je ustanovljena u pomenutim ćelijskim linijama i posle 12 časova od inokulacije u razređenju virusa od 1:64, dok je posle 24 časa od inokulacije hemadsorpcija bila prisutna i u razređenju virusa od 1:256. Posle 24 časa od inokulacije ćelijske linije Vero uzorcima virusa Newcastle bolesti, aktivisanih tripsin-versenom i dodavanja specifičnog imunog seruma, ustanovljena je inhibicija hemadsorpcije kod inokulisanih ćelija sa razređenjem imunog seruma od 1:64. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Veterinarski Glasnik T1 - Investigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle Disease virus envelope in vitro T1 - Ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti in vitro VL - 60 IS - 5-6 SP - 299 EP - 304 DO - 10.2298/VETGL0606299N ER -
@article{ author = "Nišavić, Jakov and Milić, Nenad", year = "2006", abstract = "The objective of these investigations was to examine the hemadsorption characteristics of glycoprotein HN and F antigens of the Newcastle Disease virus envelope. Six hours following inoculation of the cell line with the Newcastle Disease Vero virus, previously activated with 0.025 g/dl trypsin-versene, the appearance of hemadsorption was established in a smaller intensity in antigens diluted in ratios from 1:8 to 1:16. Hemadsorption was also determined in the mentioned cell lines 12 hours following inoculation in viruses diluted 1:64, while hemadsorption was present in virus dilutions of 1:256 after 24 hours. Inhibition of hemadsorption in inoculated cells with immune serum dilutions of 1:64 was established 24 hours following inoculation of the cell line with Vero samples of the Newcastle Disease virus, activated with trypsin-versene and the addition of specific immune serum., Cilj naših istraživanja bilo je ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti. Posle 6 časova od inokulacije ćelijske linije Vero virusom Newcastle bolesti, prethodno aktivisanim sa 0,025 g/dl tripsinversena, pojava hemadsorpcije je ustanovljena u manjem intenzitetu u razređenjima antigena od 1:8 do 1:16. Hemadsorpcija je ustanovljena u pomenutim ćelijskim linijama i posle 12 časova od inokulacije u razređenju virusa od 1:64, dok je posle 24 časa od inokulacije hemadsorpcija bila prisutna i u razređenju virusa od 1:256. Posle 24 časa od inokulacije ćelijske linije Vero uzorcima virusa Newcastle bolesti, aktivisanih tripsin-versenom i dodavanja specifičnog imunog seruma, ustanovljena je inhibicija hemadsorpcije kod inokulisanih ćelija sa razređenjem imunog seruma od 1:64.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Veterinarski Glasnik", title = "Investigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle Disease virus envelope in vitro, Ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti in vitro", volume = "60", number = "5-6", pages = "299-304", doi = "10.2298/VETGL0606299N" }
Nišavić, J.,& Milić, N.. (2006). Investigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle Disease virus envelope in vitro. in Veterinarski Glasnik Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(5-6), 299-304. https://doi.org/10.2298/VETGL0606299N
Nišavić J, Milić N. Investigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle Disease virus envelope in vitro. in Veterinarski Glasnik. 2006;60(5-6):299-304. doi:10.2298/VETGL0606299N .
Nišavić, Jakov, Milić, Nenad, "Investigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle Disease virus envelope in vitro" in Veterinarski Glasnik, 60, no. 5-6 (2006):299-304, https://doi.org/10.2298/VETGL0606299N . .