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Ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti in vitro

dc.creatorNišavić, Jakov
dc.creatorMilić, Nenad
dc.date.accessioned2020-06-03T12:58:33Z
dc.date.available2020-06-03T12:58:33Z
dc.date.issued2006
dc.identifier.issn0350-2457
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/387
dc.description.abstractThe objective of these investigations was to examine the hemadsorption characteristics of glycoprotein HN and F antigens of the Newcastle Disease virus envelope. Six hours following inoculation of the cell line with the Newcastle Disease Vero virus, previously activated with 0.025 g/dl trypsin-versene, the appearance of hemadsorption was established in a smaller intensity in antigens diluted in ratios from 1:8 to 1:16. Hemadsorption was also determined in the mentioned cell lines 12 hours following inoculation in viruses diluted 1:64, while hemadsorption was present in virus dilutions of 1:256 after 24 hours. Inhibition of hemadsorption in inoculated cells with immune serum dilutions of 1:64 was established 24 hours following inoculation of the cell line with Vero samples of the Newcastle Disease virus, activated with trypsin-versene and the addition of specific immune serum.en
dc.description.abstractCilj naših istraživanja bilo je ispitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti. Posle 6 časova od inokulacije ćelijske linije Vero virusom Newcastle bolesti, prethodno aktivisanim sa 0,025 g/dl tripsinversena, pojava hemadsorpcije je ustanovljena u manjem intenzitetu u razređenjima antigena od 1:8 do 1:16. Hemadsorpcija je ustanovljena u pomenutim ćelijskim linijama i posle 12 časova od inokulacije u razređenju virusa od 1:64, dok je posle 24 časa od inokulacije hemadsorpcija bila prisutna i u razređenju virusa od 1:256. Posle 24 časa od inokulacije ćelijske linije Vero uzorcima virusa Newcastle bolesti, aktivisanih tripsin-versenom i dodavanja specifičnog imunog seruma, ustanovljena je inhibicija hemadsorpcije kod inokulisanih ćelija sa razređenjem imunog seruma od 1:64.sr
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.rightsopenAccess
dc.sourceVeterinarski glasnik
dc.subjectNewcastle Disease virusen
dc.subjectVero cellsen
dc.subjecthemadsorptionen
dc.subjecthemadsorption inhibitionen
dc.subjectVirus Newcastle bolestisr
dc.subjectVero ćelijesr
dc.subjecthemadsorpcijasr
dc.subjectinhibicija hemadsorpcijesr
dc.titleInvestigations of hemadsorption characteristics of glycoprotein HN and F antigens of Newcastle Disease virus envelope in vitroen
dc.titleIspitivanje hemadsorpcionih svojstava glikoproteinskih HN i F antigena spoljašnjeg omotača virusa Newcastle bolesti in vitrosr
dc.typearticle
dc.rights.licenseBY
dcterms.abstractНишавић, Јаков; Милић, Ненад;
dc.citation.volume60
dc.citation.issue5-6
dc.citation.spage299
dc.citation.epage304
dc.citation.other60(5-6): 299-304
dc.identifier.doi10.2298/VETGL0606299N
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/952/386.pdf
dc.identifier.rcubconv_155
dc.type.versionpublishedVersion


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