Investigations of prevalence of antibodies to B.canis in stray dogs in territory of Belgrade
Ispitivanje prevalencije antitela na B.canis kod pasa lutalica na teritoriji grada Beograda
Апстракт
The paper covers investigations of stray dogs in the territory of the city of Belgrade. A total of 184 blood serum samples were examined for the presence of antibodies specific to Brucella canis. The method of slow agglutination in a test tube with 2- mercaptoethanol was used in the diagnostic procedure. Of the 184 examined serums, 49 (26.63%) had a titer of 1/50, 25 serums had a titer of 1/100 (13.58%), while 20 serums had a titer equal to or bigger than 1/200 (10.87%). Furthermore, 15 samples of full blood from serologiclly negative animals were also presented for isolation. The bacteriological finding for these samples was negative. The obtained results indicate that the number of seropositive stray dogs in the territory of Belgrade is extremely high and that 10.87% of the testes animals are definitely infected with Brucella canis.
Ovim radom obuhvaćeno je ispitivanje pasa lutalica na teritoriji grada Beograda. Na prisustvo antitela specifičnih na Brucella canis ukupno je ispitano 184 uzorka krvnih seruma. U dijagnostikovanju se koristila metoda spore aglutinacije u epruveti sa 2-merkaptoetanolom. Od 184 ispitana seruma, 49 (26,63%) seruma je imalo titar 1/50, 25 seruma je imalo titar 1/100 (13,58%), dok je 20 seruma imalo titar jednak ili veći od 1/200 (10,87%). Za izolaciju B. canis je dostavljeno i 15 uzoraka pune krvi od serološki negativnih životinja. Bakteriološki nalaz ovih uzoraka je bio negativan. Dobijeni rezultati ukazuju da je broj sero-pozitivnih pasa lutalica na teritoriji Beograda izuzetno visok i da je 10,87 posto testiranih pasa sigurno inficirano bakterijom Brucella canis.
Кључне речи:
Brucella canis / seroprevalence / stray dogs / agglutination / Brucella canis / seroprevalencija / psi lutalice / aglutinacijaИзвор:
Veterinarski Glasnik, 2006, 60, 1-2, 43-49Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Radojičić, Sonja AU - Ćilerdžić, Milica AU - Dimić, Bane AU - Kirćanski, Jasmina PY - 2006 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/389 AB - The paper covers investigations of stray dogs in the territory of the city of Belgrade. A total of 184 blood serum samples were examined for the presence of antibodies specific to Brucella canis. The method of slow agglutination in a test tube with 2- mercaptoethanol was used in the diagnostic procedure. Of the 184 examined serums, 49 (26.63%) had a titer of 1/50, 25 serums had a titer of 1/100 (13.58%), while 20 serums had a titer equal to or bigger than 1/200 (10.87%). Furthermore, 15 samples of full blood from serologiclly negative animals were also presented for isolation. The bacteriological finding for these samples was negative. The obtained results indicate that the number of seropositive stray dogs in the territory of Belgrade is extremely high and that 10.87% of the testes animals are definitely infected with Brucella canis. AB - Ovim radom obuhvaćeno je ispitivanje pasa lutalica na teritoriji grada Beograda. Na prisustvo antitela specifičnih na Brucella canis ukupno je ispitano 184 uzorka krvnih seruma. U dijagnostikovanju se koristila metoda spore aglutinacije u epruveti sa 2-merkaptoetanolom. Od 184 ispitana seruma, 49 (26,63%) seruma je imalo titar 1/50, 25 seruma je imalo titar 1/100 (13,58%), dok je 20 seruma imalo titar jednak ili veći od 1/200 (10,87%). Za izolaciju B. canis je dostavljeno i 15 uzoraka pune krvi od serološki negativnih životinja. Bakteriološki nalaz ovih uzoraka je bio negativan. Dobijeni rezultati ukazuju da je broj sero-pozitivnih pasa lutalica na teritoriji Beograda izuzetno visok i da je 10,87 posto testiranih pasa sigurno inficirano bakterijom Brucella canis. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Veterinarski Glasnik T1 - Investigations of prevalence of antibodies to B.canis in stray dogs in territory of Belgrade T1 - Ispitivanje prevalencije antitela na B.canis kod pasa lutalica na teritoriji grada Beograda VL - 60 IS - 1-2 SP - 43 EP - 49 DO - 10.2298/VETGL0602043R ER -
@article{ author = "Radojičić, Sonja and Ćilerdžić, Milica and Dimić, Bane and Kirćanski, Jasmina", year = "2006", abstract = "The paper covers investigations of stray dogs in the territory of the city of Belgrade. A total of 184 blood serum samples were examined for the presence of antibodies specific to Brucella canis. The method of slow agglutination in a test tube with 2- mercaptoethanol was used in the diagnostic procedure. Of the 184 examined serums, 49 (26.63%) had a titer of 1/50, 25 serums had a titer of 1/100 (13.58%), while 20 serums had a titer equal to or bigger than 1/200 (10.87%). Furthermore, 15 samples of full blood from serologiclly negative animals were also presented for isolation. The bacteriological finding for these samples was negative. The obtained results indicate that the number of seropositive stray dogs in the territory of Belgrade is extremely high and that 10.87% of the testes animals are definitely infected with Brucella canis., Ovim radom obuhvaćeno je ispitivanje pasa lutalica na teritoriji grada Beograda. Na prisustvo antitela specifičnih na Brucella canis ukupno je ispitano 184 uzorka krvnih seruma. U dijagnostikovanju se koristila metoda spore aglutinacije u epruveti sa 2-merkaptoetanolom. Od 184 ispitana seruma, 49 (26,63%) seruma je imalo titar 1/50, 25 seruma je imalo titar 1/100 (13,58%), dok je 20 seruma imalo titar jednak ili veći od 1/200 (10,87%). Za izolaciju B. canis je dostavljeno i 15 uzoraka pune krvi od serološki negativnih životinja. Bakteriološki nalaz ovih uzoraka je bio negativan. Dobijeni rezultati ukazuju da je broj sero-pozitivnih pasa lutalica na teritoriji Beograda izuzetno visok i da je 10,87 posto testiranih pasa sigurno inficirano bakterijom Brucella canis.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Veterinarski Glasnik", title = "Investigations of prevalence of antibodies to B.canis in stray dogs in territory of Belgrade, Ispitivanje prevalencije antitela na B.canis kod pasa lutalica na teritoriji grada Beograda", volume = "60", number = "1-2", pages = "43-49", doi = "10.2298/VETGL0602043R" }
Radojičić, S., Ćilerdžić, M., Dimić, B.,& Kirćanski, J.. (2006). Investigations of prevalence of antibodies to B.canis in stray dogs in territory of Belgrade. in Veterinarski Glasnik Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(1-2), 43-49. https://doi.org/10.2298/VETGL0602043R
Radojičić S, Ćilerdžić M, Dimić B, Kirćanski J. Investigations of prevalence of antibodies to B.canis in stray dogs in territory of Belgrade. in Veterinarski Glasnik. 2006;60(1-2):43-49. doi:10.2298/VETGL0602043R .
Radojičić, Sonja, Ćilerdžić, Milica, Dimić, Bane, Kirćanski, Jasmina, "Investigations of prevalence of antibodies to B.canis in stray dogs in territory of Belgrade" in Veterinarski Glasnik, 60, no. 1-2 (2006):43-49, https://doi.org/10.2298/VETGL0602043R . .